Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells

C. C. Chang, J. E. Trosko, H. J. Kung, D. Bombick, F. Matsumura

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.

Original languageEnglish
Pages (from-to)5360-5364
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number16
DOIs
Publication statusPublished - Nov 6 1985
Externally publishedYes

Fingerprint

src Genes
NIH 3T3 Cells
Protein Kinase C
Clone Cells
Uracil Nucleotides
Autoradiography
Cell Communication
Carcinogens
Agar
Transfection
Acetates
Plasmids
Genome

ASJC Scopus subject areas

  • General

Cite this

Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells. / Chang, C. C.; Trosko, J. E.; Kung, H. J.; Bombick, D.; Matsumura, F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 16, 06.11.1985, p. 5360-5364.

Research output: Contribution to journalArticle

@article{3b1b82db19c740dc8063ca218bf87506,
title = "Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells",
abstract = "The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.",
author = "Chang, {C. C.} and Trosko, {J. E.} and Kung, {H. J.} and D. Bombick and F. Matsumura",
year = "1985",
month = "11",
day = "6",
doi = "10.1073/pnas.82.16.5360",
language = "English",
volume = "82",
pages = "5360--5364",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "16",

}

TY - JOUR

T1 - Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells

AU - Chang, C. C.

AU - Trosko, J. E.

AU - Kung, H. J.

AU - Bombick, D.

AU - Matsumura, F.

PY - 1985/11/6

Y1 - 1985/11/6

N2 - The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.

AB - The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.

UR - http://www.scopus.com/inward/record.url?scp=0346804737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346804737&partnerID=8YFLogxK

U2 - 10.1073/pnas.82.16.5360

DO - 10.1073/pnas.82.16.5360

M3 - Article

C2 - 2991919

AN - SCOPUS:0346804737

VL - 82

SP - 5360

EP - 5364

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 16

ER -