Potential of a simple solid-phase extraction method coupled to analytical and bioanalytical methods for an improved determination of microcystins in algal samples

Yi Min Chen, Zong-Huei Li, Shyh Jye Lee, Jian Zhi Lin, Rang Huang, Hong Nong Chou

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix effects. To eliminate the possible interferences in the algal matrices, a new solid-phase extraction (SPE) method using silica gel as a sorbent was developed and evaluated. Results show that this SPE method could not only reduce the toxicity of the Microcystis samples towards brine shrimp by 50-80% but also eliminate 90-100% of the endogenous phosphatase activity from Spirulina and Chlorella samples, thus improving the determination of microcystins in algal samples using either of the two bioanalytical methods. The application of this SPE method as an off-line cleanup for high-performance liquid chromatography (HPLC) with UV detection is also described in this study. After SPE, the HPLC chromatograms of Microcystis samples have clear baselines that have no interferences with the analyte peaks.

Original languageEnglish
Pages (from-to)134-141
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume844
Issue number1
DOIs
Publication statusPublished - Nov 21 2006

Keywords

  • Algal dietary supplement
  • Artemia assay
  • Cyanobacteria
  • HPLC
  • Microcystin (MC)
  • Microcystis
  • Mouse Bioassay
  • Protein phosphatase assay
  • Solid-phase extraction (SPE)

ASJC Scopus subject areas

  • Biochemistry

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