Potential of a simple solid-phase extraction method coupled to analytical and bioanalytical methods for an improved determination of microcystins in algal samples

Yi Min Chen, Zong-Huei Li, Shyh Jye Lee, Jian Zhi Lin, Rang Huang, Hong Nong Chou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix effects. To eliminate the possible interferences in the algal matrices, a new solid-phase extraction (SPE) method using silica gel as a sorbent was developed and evaluated. Results show that this SPE method could not only reduce the toxicity of the Microcystis samples towards brine shrimp by 50-80% but also eliminate 90-100% of the endogenous phosphatase activity from Spirulina and Chlorella samples, thus improving the determination of microcystins in algal samples using either of the two bioanalytical methods. The application of this SPE method as an off-line cleanup for high-performance liquid chromatography (HPLC) with UV detection is also described in this study. After SPE, the HPLC chromatograms of Microcystis samples have clear baselines that have no interferences with the analyte peaks.

Original languageEnglish
Pages (from-to)134-141
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume844
Issue number1
DOIs
Publication statusPublished - Nov 21 2006

Fingerprint

Microcystins
Solid Phase Extraction
Microcystis
Artemia
Assays
High performance liquid chromatography
Toxicity
High Pressure Liquid Chromatography
Spirulina
Chlorella
Phosphoprotein Phosphatases
Silica Gel
Sorbents
Phosphoric Monoester Hydrolases
Screening

Keywords

  • Algal dietary supplement
  • Artemia assay
  • Cyanobacteria
  • HPLC
  • Microcystin (MC)
  • Microcystis
  • Mouse Bioassay
  • Protein phosphatase assay
  • Solid-phase extraction (SPE)

ASJC Scopus subject areas

  • Biochemistry

Cite this

Potential of a simple solid-phase extraction method coupled to analytical and bioanalytical methods for an improved determination of microcystins in algal samples. / Chen, Yi Min; Li, Zong-Huei; Lee, Shyh Jye; Lin, Jian Zhi; Huang, Rang; Chou, Hong Nong.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 844, No. 1, 21.11.2006, p. 134-141.

Research output: Contribution to journalArticle

@article{bd7e5479c64249efb0bcf66546e24f8e,
title = "Potential of a simple solid-phase extraction method coupled to analytical and bioanalytical methods for an improved determination of microcystins in algal samples",
abstract = "Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix effects. To eliminate the possible interferences in the algal matrices, a new solid-phase extraction (SPE) method using silica gel as a sorbent was developed and evaluated. Results show that this SPE method could not only reduce the toxicity of the Microcystis samples towards brine shrimp by 50-80{\%} but also eliminate 90-100{\%} of the endogenous phosphatase activity from Spirulina and Chlorella samples, thus improving the determination of microcystins in algal samples using either of the two bioanalytical methods. The application of this SPE method as an off-line cleanup for high-performance liquid chromatography (HPLC) with UV detection is also described in this study. After SPE, the HPLC chromatograms of Microcystis samples have clear baselines that have no interferences with the analyte peaks.",
keywords = "Algal dietary supplement, Artemia assay, Cyanobacteria, HPLC, Microcystin (MC), Microcystis, Mouse Bioassay, Protein phosphatase assay, Solid-phase extraction (SPE)",
author = "Chen, {Yi Min} and Zong-Huei Li and Lee, {Shyh Jye} and Lin, {Jian Zhi} and Rang Huang and Chou, {Hong Nong}",
year = "2006",
month = "11",
day = "21",
doi = "10.1016/j.jchromb.2006.07.014",
language = "English",
volume = "844",
pages = "134--141",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Potential of a simple solid-phase extraction method coupled to analytical and bioanalytical methods for an improved determination of microcystins in algal samples

AU - Chen, Yi Min

AU - Li, Zong-Huei

AU - Lee, Shyh Jye

AU - Lin, Jian Zhi

AU - Huang, Rang

AU - Chou, Hong Nong

PY - 2006/11/21

Y1 - 2006/11/21

N2 - Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix effects. To eliminate the possible interferences in the algal matrices, a new solid-phase extraction (SPE) method using silica gel as a sorbent was developed and evaluated. Results show that this SPE method could not only reduce the toxicity of the Microcystis samples towards brine shrimp by 50-80% but also eliminate 90-100% of the endogenous phosphatase activity from Spirulina and Chlorella samples, thus improving the determination of microcystins in algal samples using either of the two bioanalytical methods. The application of this SPE method as an off-line cleanup for high-performance liquid chromatography (HPLC) with UV detection is also described in this study. After SPE, the HPLC chromatograms of Microcystis samples have clear baselines that have no interferences with the analyte peaks.

AB - Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix effects. To eliminate the possible interferences in the algal matrices, a new solid-phase extraction (SPE) method using silica gel as a sorbent was developed and evaluated. Results show that this SPE method could not only reduce the toxicity of the Microcystis samples towards brine shrimp by 50-80% but also eliminate 90-100% of the endogenous phosphatase activity from Spirulina and Chlorella samples, thus improving the determination of microcystins in algal samples using either of the two bioanalytical methods. The application of this SPE method as an off-line cleanup for high-performance liquid chromatography (HPLC) with UV detection is also described in this study. After SPE, the HPLC chromatograms of Microcystis samples have clear baselines that have no interferences with the analyte peaks.

KW - Algal dietary supplement

KW - Artemia assay

KW - Cyanobacteria

KW - HPLC

KW - Microcystin (MC)

KW - Microcystis

KW - Mouse Bioassay

KW - Protein phosphatase assay

KW - Solid-phase extraction (SPE)

UR - http://www.scopus.com/inward/record.url?scp=33845975236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845975236&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2006.07.014

DO - 10.1016/j.jchromb.2006.07.014

M3 - Article

C2 - 16890502

AN - SCOPUS:33845975236

VL - 844

SP - 134

EP - 141

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1

ER -