Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome

Chi Hao Wu, Mei Jou Chen, Tzong Ming Shieh, Kai Lee Wang, Ya Ting Wu, Shih Min Hsia, Wenchang Chiang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study investigated the therapeutic potential of adlay on polycystic ovary syndrome (PCOS) and its possible underlying mechanism. The active anti-androgenic components in adlay were further analyzed. Rat ovarian theca cells were treated with human chorionic gonadotropin to stimulate androstenedione (AD) secretion. The ethyl acetate subfraction obtained from the ethanolic extract of adlay hull (AHE-EA) exerted superior efficacy against AD production. Four major constituents in AHE-EA, including 5,7-dihydroxychromone, liquiritigenin, isoliquiritigenin, and homoeriodictyol, were identified by HPLC-MS and exhibited strong inhibition against high AD levels. In a DHEA-induced PCOS rat model, administration of AHE-EA significantly decreased serum AD levels, improved hyperglycemia and insulin resistance, and attenuated oxidative stress and inflammatory responses in the ovaries. Histopathological morphology of ovarian tissues confirmed that AHE-EA could restore the estrus cycles and normal ovarian histology. Mechanistic characterization demonstrated that AHE-EA inhibited DHEA-induced hyperandrogenemia through modulate steroidogenic acute regulatory protein-related steroidogenesis in theca cells.

Original languageEnglish
Pages (from-to)393-406
Number of pages14
JournalJournal of Functional Foods
Volume11
Issue numberC
DOIs
Publication statusPublished - 2014

Fingerprint

polycystic ovary syndrome
Coix lacryma-jobi
Theca Cells
Hyperandrogenism
androstenedione
Androstenedione
Polycystic Ovary Syndrome
human chorionic gonadotropin
Chorionic Gonadotropin
Rodentia
rodents
prasterone
Dehydroepiandrosterone
cells
steroidogenesis
regulatory proteins
Estrus
hyperglycemia
Menstrual Cycle
insulin resistance

Keywords

  • Adlay
  • Dehydroepiandrosterone
  • Hyperandrogenemia
  • Insulin resistance
  • Polycystic ovary syndrome

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome. / Wu, Chi Hao; Chen, Mei Jou; Shieh, Tzong Ming; Wang, Kai Lee; Wu, Ya Ting; Hsia, Shih Min; Chiang, Wenchang.

In: Journal of Functional Foods, Vol. 11, No. C, 2014, p. 393-406.

Research output: Contribution to journalArticle

Wu, Chi Hao ; Chen, Mei Jou ; Shieh, Tzong Ming ; Wang, Kai Lee ; Wu, Ya Ting ; Hsia, Shih Min ; Chiang, Wenchang. / Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome. In: Journal of Functional Foods. 2014 ; Vol. 11, No. C. pp. 393-406.
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