Abstract
Objective: To investigate whether combination treatment with Taohong Siwu Decoction (桃红四物汤, TSD) and recombinant tissue-type plasminogen activator (rt-PA) potentiate in reducing infarct volume and alleviate thromboembolic stroke in an in vivo rat model.
Method: Adult male Wistar rats were subjected to embolic middle cerebral artery occlusion (MCAO) and treated with rt-PA (4 and 8 mg/kg) alone (n=5), TSD [0.7 g/(kg·day)] alone (n=5), combination of rt-PA and TSD, 24 h after stroke. Rats were sacrificed at 14 days after treatment and lesion volumes were measured. To investigate the underlying mechanism of neuroprotective effect of the combination treatment, cleaved caspase-3, tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor (HIF)-1α, and inducible nitric oxide synthase (iNOS) immunostaining were performed.
Results: Combination treatment significantly reduced infarct volume of cerebral ischemic regions compared with treatment of rt-PA and TSD alone and that of the saline control group (P
Conclusions: A combination of low-dose rt-PA and TSD after embolic stroke reduced infarct volume, improved cerebral blood flow and provided neuroprotection and these effects were associated with reduction of apoptosis and attenuation of HIF-1α, TNF-α and iNOS expression. These results provide a positive contribution to better understand the therapeutic value of the combination of TSD with rt-PA in ischemic stroke and may support further clinical evaluation.
| Original language | English |
|---|---|
| Journal | Chinese Journal of Integrative Medicine |
| DOIs | |
| Publication status | Accepted/In press - Sep 24 2014 |
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Keywords
- caspase-3
- Chinese medicine
- hypoxia-induced factor-1α
- inducible nitric oxide synthase
- rats
- recombinant tissuse-type plasminogen activator
- Taohong Siwu Decoction
- thromboembolic stroke
- tumor necrosis factor α
ASJC Scopus subject areas
- Complementary and alternative medicine
- Pharmacology (medical)
Cite this
Potential advantages of Chinese medicine Taohong Siwu Decoction (桃红四物汤) combined with tissue-plasminogen activator for alleviating middle cerebral artery occlusion-induced embolic stroke in rats. / Yen, Ting Lin; Ong, Eng Thiam; Lin, Kuan Hung; Chang, Chao Chien; Jayakumar, Thanasekaran; Lin, Song Chow; Fong, Tsorng Han; Sheu, Joen Rong.
In: Chinese Journal of Integrative Medicine, 24.09.2014.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Potential advantages of Chinese medicine Taohong Siwu Decoction (桃红四物汤) combined with tissue-plasminogen activator for alleviating middle cerebral artery occlusion-induced embolic stroke in rats
AU - Yen, Ting Lin
AU - Ong, Eng Thiam
AU - Lin, Kuan Hung
AU - Chang, Chao Chien
AU - Jayakumar, Thanasekaran
AU - Lin, Song Chow
AU - Fong, Tsorng Han
AU - Sheu, Joen Rong
PY - 2014/9/24
Y1 - 2014/9/24
N2 - Objective: To investigate whether combination treatment with Taohong Siwu Decoction (桃红四物汤, TSD) and recombinant tissue-type plasminogen activator (rt-PA) potentiate in reducing infarct volume and alleviate thromboembolic stroke in an in vivo rat model.Method: Adult male Wistar rats were subjected to embolic middle cerebral artery occlusion (MCAO) and treated with rt-PA (4 and 8 mg/kg) alone (n=5), TSD [0.7 g/(kg·day)] alone (n=5), combination of rt-PA and TSD, 24 h after stroke. Rats were sacrificed at 14 days after treatment and lesion volumes were measured. To investigate the underlying mechanism of neuroprotective effect of the combination treatment, cleaved caspase-3, tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor (HIF)-1α, and inducible nitric oxide synthase (iNOS) immunostaining were performed.Results: Combination treatment significantly reduced infarct volume of cerebral ischemic regions compared with treatment of rt-PA and TSD alone and that of the saline control group (PConclusions: A combination of low-dose rt-PA and TSD after embolic stroke reduced infarct volume, improved cerebral blood flow and provided neuroprotection and these effects were associated with reduction of apoptosis and attenuation of HIF-1α, TNF-α and iNOS expression. These results provide a positive contribution to better understand the therapeutic value of the combination of TSD with rt-PA in ischemic stroke and may support further clinical evaluation.
AB - Objective: To investigate whether combination treatment with Taohong Siwu Decoction (桃红四物汤, TSD) and recombinant tissue-type plasminogen activator (rt-PA) potentiate in reducing infarct volume and alleviate thromboembolic stroke in an in vivo rat model.Method: Adult male Wistar rats were subjected to embolic middle cerebral artery occlusion (MCAO) and treated with rt-PA (4 and 8 mg/kg) alone (n=5), TSD [0.7 g/(kg·day)] alone (n=5), combination of rt-PA and TSD, 24 h after stroke. Rats were sacrificed at 14 days after treatment and lesion volumes were measured. To investigate the underlying mechanism of neuroprotective effect of the combination treatment, cleaved caspase-3, tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor (HIF)-1α, and inducible nitric oxide synthase (iNOS) immunostaining were performed.Results: Combination treatment significantly reduced infarct volume of cerebral ischemic regions compared with treatment of rt-PA and TSD alone and that of the saline control group (PConclusions: A combination of low-dose rt-PA and TSD after embolic stroke reduced infarct volume, improved cerebral blood flow and provided neuroprotection and these effects were associated with reduction of apoptosis and attenuation of HIF-1α, TNF-α and iNOS expression. These results provide a positive contribution to better understand the therapeutic value of the combination of TSD with rt-PA in ischemic stroke and may support further clinical evaluation.
KW - caspase-3
KW - Chinese medicine
KW - hypoxia-induced factor-1α
KW - inducible nitric oxide synthase
KW - rats
KW - recombinant tissuse-type plasminogen activator
KW - Taohong Siwu Decoction
KW - thromboembolic stroke
KW - tumor necrosis factor α
UR - http://www.scopus.com/inward/record.url?scp=84908538302&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908538302&partnerID=8YFLogxK
U2 - 10.1007/s11655-014-1847-x
DO - 10.1007/s11655-014-1847-x
M3 - Article
JO - Chinese Journal of Integrative Medicine
JF - Chinese Journal of Integrative Medicine
SN - 1672-0415
ER -