Potent anti-cancer effect of 39-hydroxypterostilbene in human colon xenograft tumors

Tzu Chun Cheng, Ching Shu Lai, Min Ching Chung, Nagabhushanam Kalyanam, Muhammed Majeed, Chi Tang Ho, Yuan Soon Ho, Min Hsiung Pan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Here we report that 39-hydroxypterostilbene (HPSB), a natural pterostilbene analogue, was more potent than pterostilbene against the growth of human cancer cells (COLO 205, HCT-116, and HT-29) with measured IC50 values of 9.0, 40.2, and 70.9 mM, respectively. We found that HPSB effectively inhibited the growth of human colon cancer cells by inducing apoptosis and autophagy. Autophagy occurred at an early stage and was observed through the formation of acidic vesicular organelles and microtubule-associated protein 1 light chain 3-II production. At the molecular levels, the results from western blot analysis showed that HPSB significantly down-regulated phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) signalings including decreased the phosphorylation of mammalian target of rapamycin (mTOR). Significant therapeutic effects were demonstrated in vivo by treating nude mice bearing COLO 205 tumor xenografts with HPSB (10 mg/kg i.p.). These inhibitory effects were accompanied by mechanistic down-regulation of the protein levels of cyclooxygenase-2 (COX-2), matrix metallopeptidase-9 (MMP-9), vascular endothelial growth factor (VEGF), and cyclin D1, as well as by the induction of apoptosis in colon tumors. Our findings suggest that HPSB could serve as a novel promising agent for colon cancer treatment. This is an open-access article free of all copyright.

Original languageEnglish
Article numbere111814
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 12 2014

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human growth
autophagy
colorectal neoplasms
Heterografts
colon
Tumors
Colon
Bearings (structural)
apoptosis
Cells
Autophagy
Phosphatidylinositol 3-Kinase
Apoptosis
Colonic Neoplasms
Phosphorylation
neoplasms
Microtubule-Associated Proteins
Oncology
vascular endothelial growth factors
Cyclin D1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cheng, T. C., Lai, C. S., Chung, M. C., Kalyanam, N., Majeed, M., Ho, C. T., ... Pan, M. H. (2014). Potent anti-cancer effect of 39-hydroxypterostilbene in human colon xenograft tumors. PLoS One, 9(11), [e111814]. https://doi.org/10.1371/journal.pone.0111814

Potent anti-cancer effect of 39-hydroxypterostilbene in human colon xenograft tumors. / Cheng, Tzu Chun; Lai, Ching Shu; Chung, Min Ching; Kalyanam, Nagabhushanam; Majeed, Muhammed; Ho, Chi Tang; Ho, Yuan Soon; Pan, Min Hsiung.

In: PLoS One, Vol. 9, No. 11, e111814, 12.11.2014.

Research output: Contribution to journalArticle

Cheng, TC, Lai, CS, Chung, MC, Kalyanam, N, Majeed, M, Ho, CT, Ho, YS & Pan, MH 2014, 'Potent anti-cancer effect of 39-hydroxypterostilbene in human colon xenograft tumors', PLoS One, vol. 9, no. 11, e111814. https://doi.org/10.1371/journal.pone.0111814
Cheng TC, Lai CS, Chung MC, Kalyanam N, Majeed M, Ho CT et al. Potent anti-cancer effect of 39-hydroxypterostilbene in human colon xenograft tumors. PLoS One. 2014 Nov 12;9(11). e111814. https://doi.org/10.1371/journal.pone.0111814
Cheng, Tzu Chun ; Lai, Ching Shu ; Chung, Min Ching ; Kalyanam, Nagabhushanam ; Majeed, Muhammed ; Ho, Chi Tang ; Ho, Yuan Soon ; Pan, Min Hsiung. / Potent anti-cancer effect of 39-hydroxypterostilbene in human colon xenograft tumors. In: PLoS One. 2014 ; Vol. 9, No. 11.
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