Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water: Passive Uptake and Active Secretion by the Skin Cells

Jiun Lin Horng, Li Lu Yu, Sian Tai Liu, Po Yen Chen, Li Yih Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Molecular mechanisms of Na+, Cl-, and Ca2+ regulation in ionocytes of fish have been well investigated. However, the regulatory mechanism of K+ in fishes has been largely unknown. In this study, we investigated the mechanism of K+ regulation in medaka larvae acclimated to fresh water. Using a scanning ion-selective electrode technique (SIET) to measure the K+ fluxes at skin cells, significant K+ effluxes were found at ionocytes; in contrast, significant K+ influxes were found at the boundaries between keratinocytes. High K+ water (HK) acclimation induced the K+ effluxes at ionocytes and suppressed the K+ influxes at keratinocytes. The K+ effluxes of ionocytes were suppressed by VU591, bumetanide and ouabain. The K+ influxes of keratinocytes were suppressed by TAP. In situ hybridization analysis showed that mRNA of ROMKa was expressed by ionocytes in the skin and gills of medaka larvae. Quantitative PCR showed that mRNA levels of ROMKa and NKCC1a in gills of adult medaka were upregulated after HK acclimation. This study suggests that medaka obtain K+ through a paracellular pathway between keratinocytes and extrude K+ through ionocytes; apical ROMKa and basolateral NKCC1a are involved in the K+ secretion by ionocytes.

Original languageEnglish
Article number16215
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Oryzias latipes
keratinocytes
skin (animal)
potassium
secretion
uptake mechanisms
larvae
acclimation
gills
specific ion electrodes
cells
ouabain
fish
in situ hybridization
quantitative polymerase chain reaction
water
calcium

ASJC Scopus subject areas

  • General

Cite this

Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water : Passive Uptake and Active Secretion by the Skin Cells. / Horng, Jiun Lin; Yu, Li Lu; Liu, Sian Tai; Chen, Po Yen; Lin, Li Yih.

In: Scientific Reports, Vol. 7, No. 1, 16215, 01.12.2017.

Research output: Contribution to journalArticle

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