Possible presence of enhancing antibodies in idiopathic thrombocytopenic purpura

Yi Yuan Yang, Peter Fischer, Sy Jye Leu, Min Zhu, Virgil L. Woods, Pojen P. Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It is difficult to detect IgG anti-platelet autoantibodies in idiopathic thrombocytopenic purpura (ITP). Recently, it was reported that reactivity with glycoprotein IIb/IIIa was lost when IgG anti-GPIIb/IIIa antibodies from seven ITP patients were digested with pepsin to yield F(ab')2 fragments. These findings suggested that some IgG antiplatelet autoantibodies in ITP may be of low affinity and thus require the presence of 'enhancing' anti-IgG antibodies (i.e. rheumatoid factors, RFs) for detection. To test this hypothesis, we used a phage display technique to isolate five IgG RFs from an ITP patient (patient 1). Sequence analysis revealed that these RFs consisted of two clones, represented by GG3 and GG48. Both representative RFs bound specifically to IgG Fc fragments with apparent dissociation constants of 8.2x10-8 M and 8.8x10-7 M, respectively. Moreover, IgG RFs were subsequently found in a serum sample from patient 1. Combined, these results suggest that IgG RFs may occur in ITP, and may be required for the detection of some IgG anti-platelet autoantibodies and for the corresponding antibody- mediated platelet destruction in autoimmune ITP.

Original languageEnglish
Pages (from-to)69-80
Number of pages12
JournalBritish Journal of Haematology
Volume104
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Blocking Antibodies
Idiopathic Thrombocytopenic Purpura
Rheumatoid Factor
Immunoglobulin G
Autoantibodies
Blood Platelets
Cell Surface Display Techniques
Immunoglobulin Fc Fragments
Platelet Glycoprotein GPIIb-IIIa Complex
Antibodies
Pepsin A
Sequence Analysis
Anti-Idiotypic Antibodies
Clone Cells
anti-IgG
Serum

Keywords

  • Antiplatelet autoantibody
  • Combinatorial library
  • Enhancing antibody
  • ITP
  • Rheumatoid factor

ASJC Scopus subject areas

  • Hematology

Cite this

Possible presence of enhancing antibodies in idiopathic thrombocytopenic purpura. / Yang, Yi Yuan; Fischer, Peter; Leu, Sy Jye; Zhu, Min; Woods, Virgil L.; Chen, Pojen P.

In: British Journal of Haematology, Vol. 104, No. 1, 1999, p. 69-80.

Research output: Contribution to journalArticle

Yang, Yi Yuan ; Fischer, Peter ; Leu, Sy Jye ; Zhu, Min ; Woods, Virgil L. ; Chen, Pojen P. / Possible presence of enhancing antibodies in idiopathic thrombocytopenic purpura. In: British Journal of Haematology. 1999 ; Vol. 104, No. 1. pp. 69-80.
@article{0df8ed073b55416688413fcbd48abe50,
title = "Possible presence of enhancing antibodies in idiopathic thrombocytopenic purpura",
abstract = "It is difficult to detect IgG anti-platelet autoantibodies in idiopathic thrombocytopenic purpura (ITP). Recently, it was reported that reactivity with glycoprotein IIb/IIIa was lost when IgG anti-GPIIb/IIIa antibodies from seven ITP patients were digested with pepsin to yield F(ab')2 fragments. These findings suggested that some IgG antiplatelet autoantibodies in ITP may be of low affinity and thus require the presence of 'enhancing' anti-IgG antibodies (i.e. rheumatoid factors, RFs) for detection. To test this hypothesis, we used a phage display technique to isolate five IgG RFs from an ITP patient (patient 1). Sequence analysis revealed that these RFs consisted of two clones, represented by GG3 and GG48. Both representative RFs bound specifically to IgG Fc fragments with apparent dissociation constants of 8.2x10-8 M and 8.8x10-7 M, respectively. Moreover, IgG RFs were subsequently found in a serum sample from patient 1. Combined, these results suggest that IgG RFs may occur in ITP, and may be required for the detection of some IgG anti-platelet autoantibodies and for the corresponding antibody- mediated platelet destruction in autoimmune ITP.",
keywords = "Antiplatelet autoantibody, Combinatorial library, Enhancing antibody, ITP, Rheumatoid factor",
author = "Yang, {Yi Yuan} and Peter Fischer and Leu, {Sy Jye} and Min Zhu and Woods, {Virgil L.} and Chen, {Pojen P.}",
year = "1999",
doi = "10.1046/j.1365-2141.1999.01144.x",
language = "English",
volume = "104",
pages = "69--80",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Possible presence of enhancing antibodies in idiopathic thrombocytopenic purpura

AU - Yang, Yi Yuan

AU - Fischer, Peter

AU - Leu, Sy Jye

AU - Zhu, Min

AU - Woods, Virgil L.

AU - Chen, Pojen P.

PY - 1999

Y1 - 1999

N2 - It is difficult to detect IgG anti-platelet autoantibodies in idiopathic thrombocytopenic purpura (ITP). Recently, it was reported that reactivity with glycoprotein IIb/IIIa was lost when IgG anti-GPIIb/IIIa antibodies from seven ITP patients were digested with pepsin to yield F(ab')2 fragments. These findings suggested that some IgG antiplatelet autoantibodies in ITP may be of low affinity and thus require the presence of 'enhancing' anti-IgG antibodies (i.e. rheumatoid factors, RFs) for detection. To test this hypothesis, we used a phage display technique to isolate five IgG RFs from an ITP patient (patient 1). Sequence analysis revealed that these RFs consisted of two clones, represented by GG3 and GG48. Both representative RFs bound specifically to IgG Fc fragments with apparent dissociation constants of 8.2x10-8 M and 8.8x10-7 M, respectively. Moreover, IgG RFs were subsequently found in a serum sample from patient 1. Combined, these results suggest that IgG RFs may occur in ITP, and may be required for the detection of some IgG anti-platelet autoantibodies and for the corresponding antibody- mediated platelet destruction in autoimmune ITP.

AB - It is difficult to detect IgG anti-platelet autoantibodies in idiopathic thrombocytopenic purpura (ITP). Recently, it was reported that reactivity with glycoprotein IIb/IIIa was lost when IgG anti-GPIIb/IIIa antibodies from seven ITP patients were digested with pepsin to yield F(ab')2 fragments. These findings suggested that some IgG antiplatelet autoantibodies in ITP may be of low affinity and thus require the presence of 'enhancing' anti-IgG antibodies (i.e. rheumatoid factors, RFs) for detection. To test this hypothesis, we used a phage display technique to isolate five IgG RFs from an ITP patient (patient 1). Sequence analysis revealed that these RFs consisted of two clones, represented by GG3 and GG48. Both representative RFs bound specifically to IgG Fc fragments with apparent dissociation constants of 8.2x10-8 M and 8.8x10-7 M, respectively. Moreover, IgG RFs were subsequently found in a serum sample from patient 1. Combined, these results suggest that IgG RFs may occur in ITP, and may be required for the detection of some IgG anti-platelet autoantibodies and for the corresponding antibody- mediated platelet destruction in autoimmune ITP.

KW - Antiplatelet autoantibody

KW - Combinatorial library

KW - Enhancing antibody

KW - ITP

KW - Rheumatoid factor

UR - http://www.scopus.com/inward/record.url?scp=0032956399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032956399&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2141.1999.01144.x

DO - 10.1046/j.1365-2141.1999.01144.x

M3 - Article

VL - 104

SP - 69

EP - 80

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 1

ER -