Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion

Chen Hsin Liao, Shih Chi Yeh, Ya Hui Huang, Ruey Nan Chen, Ming Chieh Tsai, Wei Jan Chen, Hsiang Cheng Chi, Pei Ju Tai, Chia Jung Liao, Sheng Ming Wu, Wan Li Cheng, Li Mei Pai, Kwang Huei Lin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The thyroid hormone 3,3′,5-triiodo-L-thyronine (T3) regulates growth, development, and differentiation processes in animals. These activities are mediated by the nuclear thyroid hormone receptors (TRs). Microarray analyses were performed previously to study the mechanism of regulation triggered by T3 treatment in hepatoma cell lines. The results showed that spondin 2 was regulated positively by T3. However, the underlying mechanism and the physiological role of T3 in the regulation of spondin 2 are not clear. To verify the microarray results, spondin 2 was further investigated using semi-quantitative reverse transcription-PCR and western blotting. After 48 h of T3 treatment in the HepG2-TRα1#1 cell line, spondin 2 mRNA and protein levels increased by 3.9-to 5.7-fold. Similar results were observed in thyroidectomized rats. To localize the regulatory region in spondin 2, we performed serial deletions of the promoter and chromatin immunoprecipitation assays. The T3 response element on the spondin 2 promoter was localized in the -1104/-1034 or -984/-925 regions. To explore the effect of spondin 2 on cellular function, spondin 2 knockdown cell lines were established from Huh7 cells. Knockdown cells had higher migration ability and invasiveness compared with control cells. Conversely, spondin 2 overexpression in J7 cells led to lower migration ability and invasiveness compared with control cells. Furthermore, this study demonstrated that spondin 2 overexpression in some types of hepatocellular carcinomas is TR dependent. Together, these experimental findings suggest that spondin 2, which is regulated by T3, has an important role in cell invasion, cell migration, and tumor progression.

Original languageEnglish
Pages (from-to)99-111
Number of pages13
JournalEndocrine-Related Cancer
Volume17
Issue number1
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

Fingerprint

Thyroid Hormones
Cell Movement
Thyroid Hormone Receptors
Cell Line
Hepatocellular Carcinoma
Thyronines
Chromatin Immunoprecipitation
Nucleic Acid Regulatory Sequences
Response Elements
Microarray Analysis
Cytoplasmic and Nuclear Receptors
Growth and Development
Reverse Transcription
Western Blotting
Polymerase Chain Reaction
Messenger RNA
Neoplasms
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Oncology
  • Cancer Research
  • Endocrinology, Diabetes and Metabolism

Cite this

Liao, C. H., Yeh, S. C., Huang, Y. H., Chen, R. N., Tsai, M. C., Chen, W. J., ... Lin, K. H. (2010). Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion. Endocrine-Related Cancer, 17(1), 99-111. https://doi.org/10.1677/ERC-09-0050

Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion. / Liao, Chen Hsin; Yeh, Shih Chi; Huang, Ya Hui; Chen, Ruey Nan; Tsai, Ming Chieh; Chen, Wei Jan; Chi, Hsiang Cheng; Tai, Pei Ju; Liao, Chia Jung; Wu, Sheng Ming; Cheng, Wan Li; Pai, Li Mei; Lin, Kwang Huei.

In: Endocrine-Related Cancer, Vol. 17, No. 1, 03.2010, p. 99-111.

Research output: Contribution to journalArticle

Liao, CH, Yeh, SC, Huang, YH, Chen, RN, Tsai, MC, Chen, WJ, Chi, HC, Tai, PJ, Liao, CJ, Wu, SM, Cheng, WL, Pai, LM & Lin, KH 2010, 'Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion', Endocrine-Related Cancer, vol. 17, no. 1, pp. 99-111. https://doi.org/10.1677/ERC-09-0050
Liao, Chen Hsin ; Yeh, Shih Chi ; Huang, Ya Hui ; Chen, Ruey Nan ; Tsai, Ming Chieh ; Chen, Wei Jan ; Chi, Hsiang Cheng ; Tai, Pei Ju ; Liao, Chia Jung ; Wu, Sheng Ming ; Cheng, Wan Li ; Pai, Li Mei ; Lin, Kwang Huei. / Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion. In: Endocrine-Related Cancer. 2010 ; Vol. 17, No. 1. pp. 99-111.
@article{3df24cf36c52443d97addcc679c278b5,
title = "Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion",
abstract = "The thyroid hormone 3,3′,5-triiodo-L-thyronine (T3) regulates growth, development, and differentiation processes in animals. These activities are mediated by the nuclear thyroid hormone receptors (TRs). Microarray analyses were performed previously to study the mechanism of regulation triggered by T3 treatment in hepatoma cell lines. The results showed that spondin 2 was regulated positively by T3. However, the underlying mechanism and the physiological role of T3 in the regulation of spondin 2 are not clear. To verify the microarray results, spondin 2 was further investigated using semi-quantitative reverse transcription-PCR and western blotting. After 48 h of T3 treatment in the HepG2-TRα1#1 cell line, spondin 2 mRNA and protein levels increased by 3.9-to 5.7-fold. Similar results were observed in thyroidectomized rats. To localize the regulatory region in spondin 2, we performed serial deletions of the promoter and chromatin immunoprecipitation assays. The T3 response element on the spondin 2 promoter was localized in the -1104/-1034 or -984/-925 regions. To explore the effect of spondin 2 on cellular function, spondin 2 knockdown cell lines were established from Huh7 cells. Knockdown cells had higher migration ability and invasiveness compared with control cells. Conversely, spondin 2 overexpression in J7 cells led to lower migration ability and invasiveness compared with control cells. Furthermore, this study demonstrated that spondin 2 overexpression in some types of hepatocellular carcinomas is TR dependent. Together, these experimental findings suggest that spondin 2, which is regulated by T3, has an important role in cell invasion, cell migration, and tumor progression.",
author = "Liao, {Chen Hsin} and Yeh, {Shih Chi} and Huang, {Ya Hui} and Chen, {Ruey Nan} and Tsai, {Ming Chieh} and Chen, {Wei Jan} and Chi, {Hsiang Cheng} and Tai, {Pei Ju} and Liao, {Chia Jung} and Wu, {Sheng Ming} and Cheng, {Wan Li} and Pai, {Li Mei} and Lin, {Kwang Huei}",
year = "2010",
month = "3",
doi = "10.1677/ERC-09-0050",
language = "English",
volume = "17",
pages = "99--111",
journal = "Endocrine-Related Cancer",
issn = "1351-0088",
publisher = "Society for Endocrinology",
number = "1",

}

TY - JOUR

T1 - Positive regulation of spondin 2 by thyroid hormone is associated with cell migration and invasion

AU - Liao, Chen Hsin

AU - Yeh, Shih Chi

AU - Huang, Ya Hui

AU - Chen, Ruey Nan

AU - Tsai, Ming Chieh

AU - Chen, Wei Jan

AU - Chi, Hsiang Cheng

AU - Tai, Pei Ju

AU - Liao, Chia Jung

AU - Wu, Sheng Ming

AU - Cheng, Wan Li

AU - Pai, Li Mei

AU - Lin, Kwang Huei

PY - 2010/3

Y1 - 2010/3

N2 - The thyroid hormone 3,3′,5-triiodo-L-thyronine (T3) regulates growth, development, and differentiation processes in animals. These activities are mediated by the nuclear thyroid hormone receptors (TRs). Microarray analyses were performed previously to study the mechanism of regulation triggered by T3 treatment in hepatoma cell lines. The results showed that spondin 2 was regulated positively by T3. However, the underlying mechanism and the physiological role of T3 in the regulation of spondin 2 are not clear. To verify the microarray results, spondin 2 was further investigated using semi-quantitative reverse transcription-PCR and western blotting. After 48 h of T3 treatment in the HepG2-TRα1#1 cell line, spondin 2 mRNA and protein levels increased by 3.9-to 5.7-fold. Similar results were observed in thyroidectomized rats. To localize the regulatory region in spondin 2, we performed serial deletions of the promoter and chromatin immunoprecipitation assays. The T3 response element on the spondin 2 promoter was localized in the -1104/-1034 or -984/-925 regions. To explore the effect of spondin 2 on cellular function, spondin 2 knockdown cell lines were established from Huh7 cells. Knockdown cells had higher migration ability and invasiveness compared with control cells. Conversely, spondin 2 overexpression in J7 cells led to lower migration ability and invasiveness compared with control cells. Furthermore, this study demonstrated that spondin 2 overexpression in some types of hepatocellular carcinomas is TR dependent. Together, these experimental findings suggest that spondin 2, which is regulated by T3, has an important role in cell invasion, cell migration, and tumor progression.

AB - The thyroid hormone 3,3′,5-triiodo-L-thyronine (T3) regulates growth, development, and differentiation processes in animals. These activities are mediated by the nuclear thyroid hormone receptors (TRs). Microarray analyses were performed previously to study the mechanism of regulation triggered by T3 treatment in hepatoma cell lines. The results showed that spondin 2 was regulated positively by T3. However, the underlying mechanism and the physiological role of T3 in the regulation of spondin 2 are not clear. To verify the microarray results, spondin 2 was further investigated using semi-quantitative reverse transcription-PCR and western blotting. After 48 h of T3 treatment in the HepG2-TRα1#1 cell line, spondin 2 mRNA and protein levels increased by 3.9-to 5.7-fold. Similar results were observed in thyroidectomized rats. To localize the regulatory region in spondin 2, we performed serial deletions of the promoter and chromatin immunoprecipitation assays. The T3 response element on the spondin 2 promoter was localized in the -1104/-1034 or -984/-925 regions. To explore the effect of spondin 2 on cellular function, spondin 2 knockdown cell lines were established from Huh7 cells. Knockdown cells had higher migration ability and invasiveness compared with control cells. Conversely, spondin 2 overexpression in J7 cells led to lower migration ability and invasiveness compared with control cells. Furthermore, this study demonstrated that spondin 2 overexpression in some types of hepatocellular carcinomas is TR dependent. Together, these experimental findings suggest that spondin 2, which is regulated by T3, has an important role in cell invasion, cell migration, and tumor progression.

UR - http://www.scopus.com/inward/record.url?scp=77449085159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77449085159&partnerID=8YFLogxK

U2 - 10.1677/ERC-09-0050

DO - 10.1677/ERC-09-0050

M3 - Article

VL - 17

SP - 99

EP - 111

JO - Endocrine-Related Cancer

JF - Endocrine-Related Cancer

SN - 1351-0088

IS - 1

ER -