Polyvinylidene fluoride for proliferation and preservation of bovine corneal endothelial cells by enhancing type IV collagen production and deposition

Tsung Jen Wang, I. Jong Wang, Yi Hsin Chen, Jui Nan Lu, Tai Horng Young

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, biomaterials with different hydrophobic properties including polyvinyl alcohol (PVA), poly(ethylene-co-vinyl alcohol) (EVAL), tissue culture polystyrene (TCPS), and polyvinylidene fluoride (PVDF) were examined in the bovine corneal endothelial cells (BCECs) culture system to elucidate their possible impact on clinical demand and scientific interest. It was found that BCECs were inhibited to attach onto the PVA surface. Conversely, relatively more hydrophobic biomaterials EVAL, TCPS, and PVDF successfully initiate BCEC adhesion. Compared to EVAL, cultured BCECs on TCPS and PVDF exhibited higher viability. Furthermore, fibroblastic transformation on EVAL and TCPS was observed at day 17, but BCECs maintained typical hexagonal shape on the PVDF surface at day 21. This phenomenon can be rescued by previously coating type IV collagen on TCPS but not on EVAL. In addition, when BCECs were cultured on PVDF, the expressions of gap junction connexin-43, differentiation marker N-cadherin, and tight junction ZO-1 were well-developed, resembling the physiological phenotypes. After examining the type IV collagen expression by Western blot analysis and protein absorption test, a possible explanation for the better proliferation and preservation of BCECs on the PVDF substrate is that PVDF is a bioactive substratum which enables BCECs to synthesize and reserve more extracellular matrix type IV collagen, paving an important way to provide a more preferential environment for BCEC cultures. Accordingly, promoting CEC growth effects after cell-biomaterial association may be applied to the tissue engineering of corneal endothelium.

Original languageEnglish
Pages (from-to)252-260
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Collagen Type IV
Endothelial cells
Collagen
Tissue culture
Polystyrenes
Ethylene
Alcohols
Biocompatible Materials
Biomaterials
Polyvinyl Alcohol
Polyvinyl alcohols
Cell culture
polyvinylidene fluoride
Connexin 43
Differentiation Antigens
Cell adhesion
Cadherins
Tissue engineering
ethylene
Proteins

Keywords

  • corneal endothelial cells (CECs)
  • polyvinylidene fluoride (PVDF)
  • type IV collagen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Polyvinylidene fluoride for proliferation and preservation of bovine corneal endothelial cells by enhancing type IV collagen production and deposition. / Wang, Tsung Jen; Wang, I. Jong; Chen, Yi Hsin; Lu, Jui Nan; Young, Tai Horng.

In: Journal of Biomedical Materials Research - Part A, Vol. 100 A, No. 1, 01.2012, p. 252-260.

Research output: Contribution to journalArticle

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