Polysaccharide-based artificial extracellular matrix: Preparation and characterization of three-dimensional, macroporous chitosan and chondroitin sulfate composite scaffolds

Chih Kang Peng, Shu Huei Yu, Fwu Long Mi, Shin Shing Shyu

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Scaffold-guided tissue engineering based on synthetic and natural occurring polymers has gained much interest in recent years. In this article, the development of a polysaccharide-based artificial extracellular matrix (AECM) is reported. Three-dimensional, macroporous composite AECMs composed of chondroitin sulfate (ChS) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/lyophilization method. The ChS-Chito composite AECMs were crosslinked with glutaraldehyde and calcium ions (Ca2+) and cocrosslinked with N,N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The crosslinking reactions were examined with Fourier transform infrared analysis. Glutaraldehyde and Ca2+ crosslinked with Chito and ChS, respectively, to produce different types of ChS-Chito semi-interpenetrated net works. In contrast, EDC/NHS crosslinked with both Chito and ChS to produce ChS-Chito connected networks. In physiological buffer solutions, the Ca2+-crosslinked ChSChito composite AECMs showed a lower swelling ratio than their EDC/NHS- and glutaraldehyde-crosslinked counterparts. The ChS-Chito composite AECMs showed excellent antibacterial capability and biocompatibility according to the results of the in vitro antibacterial test and cytotoxic assay. This result suggested that the ChS-Chito composite AECMs might be a potential biomaterial for scaffold-guided tissue-engineering applications.

Original languageEnglish
Pages (from-to)2091-2100
Number of pages10
JournalJournal of Applied Polymer Science
Issue number5
Publication statusPublished - Feb 5 2006
Externally publishedYes



  • Artificial extracellular matrix
  • Chitosan
  • Chondritin sulfate

ASJC Scopus subject areas

  • Polymers and Plastics

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