Polysaccharide-based artificial extracellular matrix

Preparation and characterization of three-dimensional, macroporous chitosan and chondroitin sulfate composite scaffolds

Chih Kang Peng, Shu Huei Yu, Fwu Long Mi, Shin Shing Shyu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Scaffold-guided tissue engineering based on synthetic and natural occurring polymers has gained much interest in recent years. In this article, the development of a polysaccharide-based artificial extracellular matrix (AECM) is reported. Three-dimensional, macroporous composite AECMs composed of chondroitin sulfate (ChS) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/lyophilization method. The ChS-Chito composite AECMs were crosslinked with glutaraldehyde and calcium ions (Ca2+) and cocrosslinked with N,N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The crosslinking reactions were examined with Fourier transform infrared analysis. Glutaraldehyde and Ca2+ crosslinked with Chito and ChS, respectively, to produce different types of ChS-Chito semi-interpenetrated net works. In contrast, EDC/NHS crosslinked with both Chito and ChS to produce ChS-Chito connected networks. In physiological buffer solutions, the Ca2+-crosslinked ChSChito composite AECMs showed a lower swelling ratio than their EDC/NHS- and glutaraldehyde-crosslinked counterparts. The ChS-Chito composite AECMs showed excellent antibacterial capability and biocompatibility according to the results of the in vitro antibacterial test and cytotoxic assay. This result suggested that the ChS-Chito composite AECMs might be a potential biomaterial for scaffold-guided tissue-engineering applications.

Original languageEnglish
Pages (from-to)2091-2100
Number of pages10
JournalJournal of Applied Polymer Science
Volume99
Issue number5
DOIs
Publication statusPublished - Feb 5 2006
Externally publishedYes

Fingerprint

Chondroitin Sulfates
Scaffolds (biology)
Polysaccharides
Chitosan
Composite materials
Glutaral
Tissue engineering
Natural polymers
chitosan sulfate
Sulfates
Bioelectric potentials
Biocompatible Materials
Biocompatibility
Biomaterials
Crosslinking
Swelling
Calcium
Assays
Fourier transforms
Buffers

Keywords

  • Artificial extracellular matrix
  • Chitosan
  • Chondritin sulfate

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

Polysaccharide-based artificial extracellular matrix : Preparation and characterization of three-dimensional, macroporous chitosan and chondroitin sulfate composite scaffolds. / Peng, Chih Kang; Yu, Shu Huei; Mi, Fwu Long; Shyu, Shin Shing.

In: Journal of Applied Polymer Science, Vol. 99, No. 5, 05.02.2006, p. 2091-2100.

Research output: Contribution to journalArticle

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