Polysaccharide-based artificial extracellular matrix

Preparation and characterization of three-dimensional, macroporous chitosan, and heparin composite scaffold

Shu Huei Yu, Yu B. Wu, Fwu Long Mi, Shin Shing Shyu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Scaffold-guided tissue engineering based on synthetic and natural occurring polymers has gained many interests in recent year. In this study, the development of a chitosan-heparin artificial extracellular matrix (AECM) is reported. Three-dimensional, macroporous composite AECMs composed of heparin (Hep) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/ lyophilization method. The Chito-Hep composite AECMs were, respectively, crosslinked with glutaraldehyde, as well as cocrosslinked with N,N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide (EDC/NHS) and N-hydroxysuccinimide (NHS). The crosslinking reactions were examined by FT-IR analysis. In physiological buffer solution (PBS), the EDC/NHS-crosslinked Chito-Hep composite AECM showed a relative lower water retention ratio than its glutaraldehyde-crosslinked counterparts. The EDC/NHS-crosslinked Chito-Hep composite AECMs showed excellent biocompatibility, according to the results of the in vitro cytotoxic test. This result suggested that the EDC/NHS-crosslinked Chito-Hep composite AECMs might be a potential biomaterial for scaffold-guided tissue engineering applications.

Original languageEnglish
Pages (from-to)3639-3644
Number of pages6
JournalJournal of Applied Polymer Science
Volume109
Issue number6
DOIs
Publication statusPublished - Sep 15 2008
Externally publishedYes

Fingerprint

Chitosan
Scaffolds (biology)
Polysaccharides
Heparin
Composite materials
Glutaral
Tissue engineering
Natural polymers
Bioelectric potentials
Biocompatible Materials
Biocompatibility
Biomaterials
Crosslinking
Buffers
N-hydroxysuccinimide
Water

Keywords

  • Artificial extracellular matrix
  • Chitosan
  • Glutaraldehyde
  • Heparin
  • Scaffold

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Chemistry
  • Polymers and Plastics
  • Surfaces, Coatings and Films

Cite this

Polysaccharide-based artificial extracellular matrix : Preparation and characterization of three-dimensional, macroporous chitosan, and heparin composite scaffold. / Yu, Shu Huei; Wu, Yu B.; Mi, Fwu Long; Shyu, Shin Shing.

In: Journal of Applied Polymer Science, Vol. 109, No. 6, 15.09.2008, p. 3639-3644.

Research output: Contribution to journalArticle

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