Polymorphisms in one-carbon metabolism pathway genes, urinary arsenic profile, and urothelial carcinoma

Chi Jung Chung, Yeong Shiau Pu, Chien Tien Su, Hui Wen Chen, Yung Kai Huang, Horng Sheng Shiue, Yu Mei Hsueh

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Gene polymorphisms in the one-carbon metabolism pathway could contribute to arsenic methylation capability through plasma folate and homocysteine metabolism, thereby increasing the susceptibility to urothelial carcinoma (UC) risk. Objectives: The goal of our study was to evaluate the roles of gene polymorphisms in the one-carbon metabolism pathway in the carcinogenesis of UC. Methods: A hospital-based case-controlled study was conducted. The urinary arsenic profile was examined using high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Folate levels were measured using a competitive immunoassay kit. Genotyping was conducted using polymerase chain reaction-restriction fragment length polymorphism technique. Results: Patients with UC had higher urinary total arsenic, inorganic arsenic percentage (InAs%) and monomethylarsenic acid percentage (MMA%), and lower dimethylarsenic acid percentage (DMA%), plasma folate and homocysteine levels than controls. The correlations between folate and DMA%, and folate and homocysteine, were significant according to Pearson's correlation coefficients. Subjects carrying the 5,10-methylenetetrahydrofolate reductase (MTHFR) CT or TT genotype had a lower DMA% and lower folate levels than those carrying the CC genotype. Participants with the methionine synthase (MS) AA genotype had higher homocysteine levels than those with the AG or GG genotype. However, neither MTHFR nor MS gene polymorphisms were associated with UC risk. Conclusions: Environmental factors played a more important role in UC carcinogenesis than MTHFR or MS gene polymorphism.

Original languageEnglish
Pages (from-to)1605-1613
Number of pages9
JournalCancer Causes and Control
Volume21
Issue number10
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Arsenic
Folic Acid
Carbon
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Homocysteine
Carcinoma
Methylenetetrahydrofolate Reductase (NADPH2)
Genotype
Genes
Carcinogenesis
Acids
Immunoassay
Restriction Fragment Length Polymorphisms
Methylation
Spectrum Analysis
High Pressure Liquid Chromatography
Polymerase Chain Reaction

Keywords

  • CBS
  • MS
  • MTHFR
  • One-carbon metabolism
  • Polymorphism
  • SNP
  • Urinary arsenic
  • Urothelial carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Polymorphisms in one-carbon metabolism pathway genes, urinary arsenic profile, and urothelial carcinoma. / Chung, Chi Jung; Pu, Yeong Shiau; Su, Chien Tien; Chen, Hui Wen; Huang, Yung Kai; Shiue, Horng Sheng; Hsueh, Yu Mei.

In: Cancer Causes and Control, Vol. 21, No. 10, 10.2010, p. 1605-1613.

Research output: Contribution to journalArticle

Chung, Chi Jung ; Pu, Yeong Shiau ; Su, Chien Tien ; Chen, Hui Wen ; Huang, Yung Kai ; Shiue, Horng Sheng ; Hsueh, Yu Mei. / Polymorphisms in one-carbon metabolism pathway genes, urinary arsenic profile, and urothelial carcinoma. In: Cancer Causes and Control. 2010 ; Vol. 21, No. 10. pp. 1605-1613.
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AB - Background: Gene polymorphisms in the one-carbon metabolism pathway could contribute to arsenic methylation capability through plasma folate and homocysteine metabolism, thereby increasing the susceptibility to urothelial carcinoma (UC) risk. Objectives: The goal of our study was to evaluate the roles of gene polymorphisms in the one-carbon metabolism pathway in the carcinogenesis of UC. Methods: A hospital-based case-controlled study was conducted. The urinary arsenic profile was examined using high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Folate levels were measured using a competitive immunoassay kit. Genotyping was conducted using polymerase chain reaction-restriction fragment length polymorphism technique. Results: Patients with UC had higher urinary total arsenic, inorganic arsenic percentage (InAs%) and monomethylarsenic acid percentage (MMA%), and lower dimethylarsenic acid percentage (DMA%), plasma folate and homocysteine levels than controls. The correlations between folate and DMA%, and folate and homocysteine, were significant according to Pearson's correlation coefficients. Subjects carrying the 5,10-methylenetetrahydrofolate reductase (MTHFR) CT or TT genotype had a lower DMA% and lower folate levels than those carrying the CC genotype. Participants with the methionine synthase (MS) AA genotype had higher homocysteine levels than those with the AG or GG genotype. However, neither MTHFR nor MS gene polymorphisms were associated with UC risk. Conclusions: Environmental factors played a more important role in UC carcinogenesis than MTHFR or MS gene polymorphism.

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