Polymorphisms and intron sequences flanking the alternatively spliced 8-amino-acid exon of γ2 subunit gene for GABAA receptors

Andrew Tai Ann Cheng, El Wui Loh, Chee Yu Cheng, Yuan Chun Wang, Yun Pung Paul Hsu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Gamma-amminobutyric acid (GABA) is a major inhibitory neurotransmitter. Two alternatively spliced forms of the γ2 subunit of GABAA receptor (γ2L and γ2S), which differ by an exon of eight amino acids, show different sensitivities to modulatory effects of ethanol on receptor activities. A 2.7 kb DNA fragment and an 1.7 kb DNA fragment covering respectively the introns upstream and downstream from the 8-amino-acid exon were obtained through PCR amplification of human genomic DNA using primers derived from cDNA sequences. Total sequencing of these fragments showed a composite 4.2 kb segment containing the 8-amino-acid exon and consensus sequences for RNA splice junctions. Restriction fragment length polymorphisms (RFLP) based on NciI restriction digestion were found among Chinese in Taiwan. This RFLP provides a useful DNA marker for allelic association or linkage analyses of the role of GABAA receptors in predisposition to alcoholism or other neuropsychiatric disorders.

Original languageEnglish
Pages (from-to)683-685
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume238
Issue number2
DOIs
Publication statusPublished - Sep 18 1997
Externally publishedYes

Fingerprint

GABA-A Receptors
Polymorphism
Introns
Exons
Genes
Amino Acids
Restriction Fragment Length Polymorphisms
DNA
DNA Primers
Consensus Sequence
Taiwan
Genetic Markers
Alcoholism
Amplification
Neurotransmitter Agents
Digestion
Amino Acid Sequence
Ethanol
Complementary DNA
RNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Polymorphisms and intron sequences flanking the alternatively spliced 8-amino-acid exon of γ2 subunit gene for GABAA receptors. / Cheng, Andrew Tai Ann; Loh, El Wui; Cheng, Chee Yu; Wang, Yuan Chun; Hsu, Yun Pung Paul.

In: Biochemical and Biophysical Research Communications, Vol. 238, No. 2, 18.09.1997, p. 683-685.

Research output: Contribution to journalArticle

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AU - Hsu, Yun Pung Paul

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