Polygonum viviparum L. inhibits the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through haem oxygenase-1 induction and activation of the Nrf2 pathway

Hui Wen Cheng, Kock Chee Lee, Khoot Peng Cheah, Ming Long Chang, Che-Wei Lin, Joe Sharg Li, Wen Yu Yu, E. Tung Liu, Chien Ming Hu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Polygonum viviparum L. (PV) is a member of the family Polygonaceae and is widely distributed in high-elevation areas. It is used as a folk remedy to treat inflammation-related diseases. This study was focused on the anti-inflammatory response of PV against lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. Results: Treatment with PV did not cause cytotoxicity at 0-50 μg mL-1 in RAW264.7 macrophages, and the IC50 value was 270 μg mL-1. PV inhibited LPS-stimulated nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)-1β and tumour necrosis factor (TNF)-α release and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. In addition, PV suppressed the LPS-induced p65 expression of nuclear factor (NF)-κB, which is associated with the inhibition of IκB-α degradation. These results suggest that, among mechanisms of the anti-inflammatory response, PV inhibits the production of NO and these cytokines by down-regulating iNOS and COX-2 gene expression. Furthermore, PV can induce haem oxygenase (HO)-1 protein expression through nuclear factor E2-related factor 2 (Nrf2) activation. A specific inhibitor of HO-1, zinc(II) protoporphyrin IX, inhibited the suppression of iNOS and COX-2 expression by PV. Conclusion: These results suggest that PV possesses anti-inflammatory actions in macrophages and works through a novel mechanism involving Nrf2 actions and HO-1. Thus PV could be considered for application as a potential therapeutic approach for inflammation-associated disorders.

Original languageEnglish
Pages (from-to)491-497
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume93
Issue number3
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Bistorta vivipara
NF-E2-Related Factor 2
Polygonum
Heme Oxygenase (Decyclizing)
oxygenases
lipopolysaccharides
Lipopolysaccharides
macrophages
inflammation
Macrophages
prostaglandin synthase
Cyclooxygenase 2
Anti-Inflammatory Agents
Inflammation
Nitric Oxide Synthase
nitric oxide
Nitric Oxide
Polygonaceae
protein synthesis
protoporphyrin

Keywords

  • Haem oxygenase-1
  • Lipopolysaccharide
  • Nuclear factor E2-related factor 2
  • Polygonum viviparum L
  • RAW264.7 macrophages

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Nutrition and Dietetics
  • Biotechnology

Cite this

Polygonum viviparum L. inhibits the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through haem oxygenase-1 induction and activation of the Nrf2 pathway. / Cheng, Hui Wen; Lee, Kock Chee; Cheah, Khoot Peng; Chang, Ming Long; Lin, Che-Wei; Li, Joe Sharg; Yu, Wen Yu; Liu, E. Tung; Hu, Chien Ming.

In: Journal of the Science of Food and Agriculture, Vol. 93, No. 3, 02.2013, p. 491-497.

Research output: Contribution to journalArticle

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abstract = "Background: Polygonum viviparum L. (PV) is a member of the family Polygonaceae and is widely distributed in high-elevation areas. It is used as a folk remedy to treat inflammation-related diseases. This study was focused on the anti-inflammatory response of PV against lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. Results: Treatment with PV did not cause cytotoxicity at 0-50 μg mL-1 in RAW264.7 macrophages, and the IC50 value was 270 μg mL-1. PV inhibited LPS-stimulated nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)-1β and tumour necrosis factor (TNF)-α release and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. In addition, PV suppressed the LPS-induced p65 expression of nuclear factor (NF)-κB, which is associated with the inhibition of IκB-α degradation. These results suggest that, among mechanisms of the anti-inflammatory response, PV inhibits the production of NO and these cytokines by down-regulating iNOS and COX-2 gene expression. Furthermore, PV can induce haem oxygenase (HO)-1 protein expression through nuclear factor E2-related factor 2 (Nrf2) activation. A specific inhibitor of HO-1, zinc(II) protoporphyrin IX, inhibited the suppression of iNOS and COX-2 expression by PV. Conclusion: These results suggest that PV possesses anti-inflammatory actions in macrophages and works through a novel mechanism involving Nrf2 actions and HO-1. Thus PV could be considered for application as a potential therapeutic approach for inflammation-associated disorders.",
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T1 - Polygonum viviparum L. inhibits the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through haem oxygenase-1 induction and activation of the Nrf2 pathway

AU - Cheng, Hui Wen

AU - Lee, Kock Chee

AU - Cheah, Khoot Peng

AU - Chang, Ming Long

AU - Lin, Che-Wei

AU - Li, Joe Sharg

AU - Yu, Wen Yu

AU - Liu, E. Tung

AU - Hu, Chien Ming

PY - 2013/2

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N2 - Background: Polygonum viviparum L. (PV) is a member of the family Polygonaceae and is widely distributed in high-elevation areas. It is used as a folk remedy to treat inflammation-related diseases. This study was focused on the anti-inflammatory response of PV against lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. Results: Treatment with PV did not cause cytotoxicity at 0-50 μg mL-1 in RAW264.7 macrophages, and the IC50 value was 270 μg mL-1. PV inhibited LPS-stimulated nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)-1β and tumour necrosis factor (TNF)-α release and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. In addition, PV suppressed the LPS-induced p65 expression of nuclear factor (NF)-κB, which is associated with the inhibition of IκB-α degradation. These results suggest that, among mechanisms of the anti-inflammatory response, PV inhibits the production of NO and these cytokines by down-regulating iNOS and COX-2 gene expression. Furthermore, PV can induce haem oxygenase (HO)-1 protein expression through nuclear factor E2-related factor 2 (Nrf2) activation. A specific inhibitor of HO-1, zinc(II) protoporphyrin IX, inhibited the suppression of iNOS and COX-2 expression by PV. Conclusion: These results suggest that PV possesses anti-inflammatory actions in macrophages and works through a novel mechanism involving Nrf2 actions and HO-1. Thus PV could be considered for application as a potential therapeutic approach for inflammation-associated disorders.

AB - Background: Polygonum viviparum L. (PV) is a member of the family Polygonaceae and is widely distributed in high-elevation areas. It is used as a folk remedy to treat inflammation-related diseases. This study was focused on the anti-inflammatory response of PV against lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. Results: Treatment with PV did not cause cytotoxicity at 0-50 μg mL-1 in RAW264.7 macrophages, and the IC50 value was 270 μg mL-1. PV inhibited LPS-stimulated nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)-1β and tumour necrosis factor (TNF)-α release and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. In addition, PV suppressed the LPS-induced p65 expression of nuclear factor (NF)-κB, which is associated with the inhibition of IκB-α degradation. These results suggest that, among mechanisms of the anti-inflammatory response, PV inhibits the production of NO and these cytokines by down-regulating iNOS and COX-2 gene expression. Furthermore, PV can induce haem oxygenase (HO)-1 protein expression through nuclear factor E2-related factor 2 (Nrf2) activation. A specific inhibitor of HO-1, zinc(II) protoporphyrin IX, inhibited the suppression of iNOS and COX-2 expression by PV. Conclusion: These results suggest that PV possesses anti-inflammatory actions in macrophages and works through a novel mechanism involving Nrf2 actions and HO-1. Thus PV could be considered for application as a potential therapeutic approach for inflammation-associated disorders.

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