Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells

Yu Chi Wang, Ru Huei Fu, Hsyue Jen Hsieh, Hsiang Tai Chao, Shu Huei Kao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To induce apoptosis of endometriotic cells of patients with endometriosis. Design: To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis. Setting: University hospital infertility center. Patient(s): Twelve women who visited the center for infertility therapy. Intervention(s): Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment. Main Outcome Measure(s): Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining. Result(s): The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 μg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 μg of polyglycolic acid/chitosan glue A. Conclusion(s): This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalFertility and Sterility
Volume84
Issue number1
DOIs
Publication statusPublished - Jul 2005

Fingerprint

Polyglycolic Acid
Chitosan
Adhesives
Apoptosis
Endometriosis
Infertility
Cell Proliferation
Primary Cell Culture
Propidium
Annexin A5
Therapeutics
Caspases
Fluorescein
Caspase 3
Reaction Time
Flow Cytometry
Outcome Assessment (Health Care)
Staining and Labeling

Keywords

  • Apoptosis
  • Chitosan
  • Endometriosis
  • Polyglycolic acid

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells. / Wang, Yu Chi; Fu, Ru Huei; Hsieh, Hsyue Jen; Chao, Hsiang Tai; Kao, Shu Huei.

In: Fertility and Sterility, Vol. 84, No. 1, 07.2005, p. 75-81.

Research output: Contribution to journalArticle

Wang, Yu Chi ; Fu, Ru Huei ; Hsieh, Hsyue Jen ; Chao, Hsiang Tai ; Kao, Shu Huei. / Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells. In: Fertility and Sterility. 2005 ; Vol. 84, No. 1. pp. 75-81.
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AB - Objective: To induce apoptosis of endometriotic cells of patients with endometriosis. Design: To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis. Setting: University hospital infertility center. Patient(s): Twelve women who visited the center for infertility therapy. Intervention(s): Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment. Main Outcome Measure(s): Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining. Result(s): The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 μg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 μg of polyglycolic acid/chitosan glue A. Conclusion(s): This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy.

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