Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Wen Chang Chang, Muh Lin Lee, Chen Kung Chou, Sheng Chung Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

Original languageEnglish
Pages (from-to)342-344
Number of pages3
JournalAnalytical Biochemistry
Volume132
Issue number2
DOIs
Publication statusPublished - Jul 15 1983
Externally publishedYes

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Keywords

  • phosphoamino acids
  • polyamide
  • TLC

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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