Pleomorphic extra-renal manifestation of the glomerular podocyte marker podocalyxin in tissues of normal beagle dogs

Wei Ling Lin, Victor Fei Pang, Chen Hsuan Liu, Jen Yau Chen, Kuo Fang Shen, Yu Yu Lin, Chun Ying Yu, Yung Ho Hsu, Tzuu Shuh Jou

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Podocalyxin (PC) was initially identified as a major sialoprotein on the apical surface of glomerular podocytes to perform the filtration barrier function. Later, it was reported to be expressed in endothelial cells, megakaryotes/platelets, and hemangioblasts, the common progenitor cells of the hematopoietic and endothelial cells. Recently, increasing numbers of reports have indicated that PC is not merely a molecule restricted at renal glomerulus, angiogenic or hematopoietic system. To further elucidate the expression pattern and address the possible physiological role of PC in adult mammals, we conducted an extensive study by immunohistochemistry and immunofluorescence staining on various tissues of healthy adult beagle dogs. By combinatory usage of two different anti-podocalyxin antibodies recognizing distinct epitopes in PC, we have demonstrated that (1) PC is expressed in renal tubules, mesothelium, myocardium, striated muscles in tongue, esophagus and extraocular region, myoepithelial cells in esophagus and salivary glands, neurons, and ependyma, etc.; (2) there are at least three forms of PC proteins, depending upon the accessibility of two different PC antibodies, expressed in different organs/systems; and (3) a particular form of PC is distributed in a vesicle-like compartment in certain organs/systems, such as the central nervous system.

Original languageEnglish
Pages (from-to)399-414
Number of pages16
JournalHistochemistry and Cell Biology
Volume127
Issue number4
DOIs
Publication statusPublished - Apr 2007

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esophagus
Podocytes
dogs
Endothelial cells
antibodies
Antibodies
organs
markers
hematopoietic system
glomerulus
Dogs
Tissue
Kidney
salivary glands
Epitopes
myocardium
skeletal muscle
glands
central nervous system
mammals

Keywords

  • Gp135
  • Immunohistochemistry
  • Podocalyxin
  • Sialomucin

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Pleomorphic extra-renal manifestation of the glomerular podocyte marker podocalyxin in tissues of normal beagle dogs. / Lin, Wei Ling; Pang, Victor Fei; Liu, Chen Hsuan; Chen, Jen Yau; Shen, Kuo Fang; Lin, Yu Yu; Yu, Chun Ying; Hsu, Yung Ho; Jou, Tzuu Shuh.

In: Histochemistry and Cell Biology, Vol. 127, No. 4, 04.2007, p. 399-414.

Research output: Contribution to journalArticle

Lin, Wei Ling ; Pang, Victor Fei ; Liu, Chen Hsuan ; Chen, Jen Yau ; Shen, Kuo Fang ; Lin, Yu Yu ; Yu, Chun Ying ; Hsu, Yung Ho ; Jou, Tzuu Shuh. / Pleomorphic extra-renal manifestation of the glomerular podocyte marker podocalyxin in tissues of normal beagle dogs. In: Histochemistry and Cell Biology. 2007 ; Vol. 127, No. 4. pp. 399-414.
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