Platonin preserves blood-brain barrier integrity in septic rats

Chia Tse Yeh, Ming Chang Kao, Cay Huyen Chen, Chun Jen Huang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalActa Anaesthesiologica Taiwanica
Volume53
Issue number1
DOIs
Publication statusPublished - Mar 1 2015
Externally publishedYes

Fingerprint

Blood-Brain Barrier
Punctures
Ligation
Evans Blue
Brain Edema
Inflammation
Malondialdehyde
Permeability
Interleukin-6
Sepsis
Coloring Agents
Claudin-5
platonin
Staining and Labeling
Tight Junction Proteins
Oxidative Stress
Anti-Inflammatory Agents

Keywords

  • cecal ligation and puncture
  • Evans blue dye
  • inflammation
  • rat
  • tight junction

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Platonin preserves blood-brain barrier integrity in septic rats. / Yeh, Chia Tse; Kao, Ming Chang; Chen, Cay Huyen; Huang, Chun Jen.

In: Acta Anaesthesiologica Taiwanica, Vol. 53, No. 1, 01.03.2015, p. 12-15.

Research output: Contribution to journalArticle

Yeh, Chia Tse ; Kao, Ming Chang ; Chen, Cay Huyen ; Huang, Chun Jen. / Platonin preserves blood-brain barrier integrity in septic rats. In: Acta Anaesthesiologica Taiwanica. 2015 ; Vol. 53, No. 1. pp. 12-15.
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abstract = "Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.",
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N2 - Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.

AB - Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.

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