8 Citations (Scopus)

Abstract

Background: We previously have shown that platonin, a potent antioxidant, significantly attenuated inflammatory molecules up-regulation in RAW264.7 cells, a murine macrophage-like cell line. Inflammatory molecules expression is under the regulation of activator protein-1 (AP-1), a crucial transcription factor and a heterodimeric protein that composes of proteins from c-Jun and c-Fos families. AP-1 expression is regulated by mitogen-activated protein kinases (MAPKs). We sought to elucidate the effects of platonin on MAPKs and AP-1 up-regulation in activated RAW264.7 cells. Materials and Methods: RAW264.7 cells were allocated to receive phosphate buffered saline, lipopolysaccharide (LPS, 100 ng/mL), platonin (100μM), or platonin plus LPS and designated as the PBS, LPS, platonin, or LPS + platonin group, respectively. After harvesting, expression of the investigated molecules was evaluated. Results: The cytosolic protein concentrations of MAPKs, including extracellular regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK, of the LPS group were significantly higher than those of the PBS and platonin groups. The nuclear protein concentrations of AP-1, including c-Jun and c-Fos, and the AP-1-DNA binding activity of the LPS group were also significantly higher than those of the PBS and platonin groups. In contrast, the concentrations of ERK, JNK, and p38 MAPK of the LPS + platonin group were significantly lower than those of the LPS group. Moreover, the concentrations of c-Jun and c-Fos and the AP-1-DNA binding activity of the LPS + platonin group were significantly lower than those of the LPS group. Conclusions: Platonin significantly attenuated MAPKs and AP-1 upregulation in activated RAW264.7 cells.

Original languageEnglish
JournalJournal of Surgical Research
Volume167
Issue number2
DOIs
Publication statusPublished - May 15 2011

Fingerprint

Transcription Factor AP-1
Mitogen-Activated Protein Kinases
Endotoxins
Up-Regulation
Proto-Oncogene Proteins c-fos
Phosphotransferases
p38 Mitogen-Activated Protein Kinases
platonin
Proto-Oncogene Proteins c-jun
JNK Mitogen-Activated Protein Kinases
DNA
Nuclear Proteins
Lipopolysaccharides
Proteins
Transcription Factors
Antioxidants
Macrophages
Phosphates
Cell Line

Keywords

  • c-Fos
  • c-Jun
  • LPS binding
  • oxidative stress
  • RAW264.7

ASJC Scopus subject areas

  • Surgery

Cite this

Platonin inhibits endotoxin-induced MAPK and AP-1 up-regulation. / Lee, Jie Jen; Liu, Chien Liang; Tsai, Pei Shan; Yang, Chih Lin; Lao, Hsuan Chih; Huang, Chun Jen.

In: Journal of Surgical Research, Vol. 167, No. 2, 15.05.2011.

Research output: Contribution to journalArticle

Lee, Jie Jen ; Liu, Chien Liang ; Tsai, Pei Shan ; Yang, Chih Lin ; Lao, Hsuan Chih ; Huang, Chun Jen. / Platonin inhibits endotoxin-induced MAPK and AP-1 up-regulation. In: Journal of Surgical Research. 2011 ; Vol. 167, No. 2.
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title = "Platonin inhibits endotoxin-induced MAPK and AP-1 up-regulation",
abstract = "Background: We previously have shown that platonin, a potent antioxidant, significantly attenuated inflammatory molecules up-regulation in RAW264.7 cells, a murine macrophage-like cell line. Inflammatory molecules expression is under the regulation of activator protein-1 (AP-1), a crucial transcription factor and a heterodimeric protein that composes of proteins from c-Jun and c-Fos families. AP-1 expression is regulated by mitogen-activated protein kinases (MAPKs). We sought to elucidate the effects of platonin on MAPKs and AP-1 up-regulation in activated RAW264.7 cells. Materials and Methods: RAW264.7 cells were allocated to receive phosphate buffered saline, lipopolysaccharide (LPS, 100 ng/mL), platonin (100μM), or platonin plus LPS and designated as the PBS, LPS, platonin, or LPS + platonin group, respectively. After harvesting, expression of the investigated molecules was evaluated. Results: The cytosolic protein concentrations of MAPKs, including extracellular regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK, of the LPS group were significantly higher than those of the PBS and platonin groups. The nuclear protein concentrations of AP-1, including c-Jun and c-Fos, and the AP-1-DNA binding activity of the LPS group were also significantly higher than those of the PBS and platonin groups. In contrast, the concentrations of ERK, JNK, and p38 MAPK of the LPS + platonin group were significantly lower than those of the LPS group. Moreover, the concentrations of c-Jun and c-Fos and the AP-1-DNA binding activity of the LPS + platonin group were significantly lower than those of the LPS group. Conclusions: Platonin significantly attenuated MAPKs and AP-1 upregulation in activated RAW264.7 cells.",
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author = "Lee, {Jie Jen} and Liu, {Chien Liang} and Tsai, {Pei Shan} and Yang, {Chih Lin} and Lao, {Hsuan Chih} and Huang, {Chun Jen}",
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T1 - Platonin inhibits endotoxin-induced MAPK and AP-1 up-regulation

AU - Lee, Jie Jen

AU - Liu, Chien Liang

AU - Tsai, Pei Shan

AU - Yang, Chih Lin

AU - Lao, Hsuan Chih

AU - Huang, Chun Jen

PY - 2011/5/15

Y1 - 2011/5/15

N2 - Background: We previously have shown that platonin, a potent antioxidant, significantly attenuated inflammatory molecules up-regulation in RAW264.7 cells, a murine macrophage-like cell line. Inflammatory molecules expression is under the regulation of activator protein-1 (AP-1), a crucial transcription factor and a heterodimeric protein that composes of proteins from c-Jun and c-Fos families. AP-1 expression is regulated by mitogen-activated protein kinases (MAPKs). We sought to elucidate the effects of platonin on MAPKs and AP-1 up-regulation in activated RAW264.7 cells. Materials and Methods: RAW264.7 cells were allocated to receive phosphate buffered saline, lipopolysaccharide (LPS, 100 ng/mL), platonin (100μM), or platonin plus LPS and designated as the PBS, LPS, platonin, or LPS + platonin group, respectively. After harvesting, expression of the investigated molecules was evaluated. Results: The cytosolic protein concentrations of MAPKs, including extracellular regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK, of the LPS group were significantly higher than those of the PBS and platonin groups. The nuclear protein concentrations of AP-1, including c-Jun and c-Fos, and the AP-1-DNA binding activity of the LPS group were also significantly higher than those of the PBS and platonin groups. In contrast, the concentrations of ERK, JNK, and p38 MAPK of the LPS + platonin group were significantly lower than those of the LPS group. Moreover, the concentrations of c-Jun and c-Fos and the AP-1-DNA binding activity of the LPS + platonin group were significantly lower than those of the LPS group. Conclusions: Platonin significantly attenuated MAPKs and AP-1 upregulation in activated RAW264.7 cells.

AB - Background: We previously have shown that platonin, a potent antioxidant, significantly attenuated inflammatory molecules up-regulation in RAW264.7 cells, a murine macrophage-like cell line. Inflammatory molecules expression is under the regulation of activator protein-1 (AP-1), a crucial transcription factor and a heterodimeric protein that composes of proteins from c-Jun and c-Fos families. AP-1 expression is regulated by mitogen-activated protein kinases (MAPKs). We sought to elucidate the effects of platonin on MAPKs and AP-1 up-regulation in activated RAW264.7 cells. Materials and Methods: RAW264.7 cells were allocated to receive phosphate buffered saline, lipopolysaccharide (LPS, 100 ng/mL), platonin (100μM), or platonin plus LPS and designated as the PBS, LPS, platonin, or LPS + platonin group, respectively. After harvesting, expression of the investigated molecules was evaluated. Results: The cytosolic protein concentrations of MAPKs, including extracellular regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK, of the LPS group were significantly higher than those of the PBS and platonin groups. The nuclear protein concentrations of AP-1, including c-Jun and c-Fos, and the AP-1-DNA binding activity of the LPS group were also significantly higher than those of the PBS and platonin groups. In contrast, the concentrations of ERK, JNK, and p38 MAPK of the LPS + platonin group were significantly lower than those of the LPS group. Moreover, the concentrations of c-Jun and c-Fos and the AP-1-DNA binding activity of the LPS + platonin group were significantly lower than those of the LPS group. Conclusions: Platonin significantly attenuated MAPKs and AP-1 upregulation in activated RAW264.7 cells.

KW - c-Fos

KW - c-Jun

KW - LPS binding

KW - oxidative stress

KW - RAW264.7

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