Platonin attenuates LPS-induced CAT-2 and CAT-2B induction in stimulated murine macrophages

C. C. Chen, J. J. Lee, P. S. Tsai, Y. T. Lu, C. L. Huang, C. J. Huang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Platonin, a cyanine photosensitizing dye, is a potent immunomodulator that suppresses acute inflammation. Platonin not only inhibits interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α production but also improves circulatory failure in septic rats. In addition, platonin reduces plasma nitric oxide (NO) formation during sepsis. However, the effects of platonin on inducible NO synthase (iNOS) and cationic amino-acid transporter (including CAT-2, CAT-2 A, and CAT-2B) expressions during sepsis remain uninvestigated. Methods: Five groups of confluent murine macrophages (RAW264.7 cells) were randomly allocated to receive a 1-h pretreatment of one of five doses of platonin (0.1 μM, 1 μM, 10 μM, 100 μM, or 1000 μM) followed by lipopolysaccharide (LPS; 100 ng ml-1). For negative, positive, and platonin control, three other groups of cell cultures were randomly allocated to receive phosphate-buffered saline, LPS, or platonin (1000 μM). The cultures were harvested after exposing them to LPS for 18 h or a comparable duration in those groups without LPS. NO production, L-arginine transport, and expression of the relevant enzymes were then evaluated. Results: Platonin significantly attenuated LPS-induced up-regulation of iNOS expression and NO production in stimulated murine macrophages in a dose-dependent manner. Platonin also significantly inhibited up-regulation of CAT-2 and CAT-2B expression as well as L-arginine transport in LPS-stimulated murine macrophages in a dose-dependent manner. In contrast, CAT-2 A expression in murine macrophages was not affected by LPS and/or platonin. Conclusions: Platonin attenuates NO production and L-arginine transport in LPS-stimulated murine macrophages possibly through inhibiting iNOS, CAT-2, and CAT-2B expression.

Original languageEnglish
Pages (from-to)604-612
Number of pages9
JournalActa Anaesthesiologica Scandinavica
Volume50
Issue number5
DOIs
Publication statusPublished - May 2006

Fingerprint

Macrophages
Nitric Oxide
Arginine
Nitric Oxide Synthase
Sepsis
Basic Amino Acid Transport Systems
platonin
Up-Regulation
Immunologic Factors
Nitric Oxide Synthase Type II
Interleukin-1
Lipopolysaccharides
Shock
Interleukin-6
Coloring Agents
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Phosphates
Inflammation
Enzymes

Keywords

  • CAT-2
  • iNOS
  • L-arginine
  • LPS
  • Macrophages
  • Nitric oxide
  • Platonin

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Platonin attenuates LPS-induced CAT-2 and CAT-2B induction in stimulated murine macrophages. / Chen, C. C.; Lee, J. J.; Tsai, P. S.; Lu, Y. T.; Huang, C. L.; Huang, C. J.

In: Acta Anaesthesiologica Scandinavica, Vol. 50, No. 5, 05.2006, p. 604-612.

Research output: Contribution to journalArticle

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T1 - Platonin attenuates LPS-induced CAT-2 and CAT-2B induction in stimulated murine macrophages

AU - Chen, C. C.

AU - Lee, J. J.

AU - Tsai, P. S.

AU - Lu, Y. T.

AU - Huang, C. L.

AU - Huang, C. J.

PY - 2006/5

Y1 - 2006/5

N2 - Background: Platonin, a cyanine photosensitizing dye, is a potent immunomodulator that suppresses acute inflammation. Platonin not only inhibits interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α production but also improves circulatory failure in septic rats. In addition, platonin reduces plasma nitric oxide (NO) formation during sepsis. However, the effects of platonin on inducible NO synthase (iNOS) and cationic amino-acid transporter (including CAT-2, CAT-2 A, and CAT-2B) expressions during sepsis remain uninvestigated. Methods: Five groups of confluent murine macrophages (RAW264.7 cells) were randomly allocated to receive a 1-h pretreatment of one of five doses of platonin (0.1 μM, 1 μM, 10 μM, 100 μM, or 1000 μM) followed by lipopolysaccharide (LPS; 100 ng ml-1). For negative, positive, and platonin control, three other groups of cell cultures were randomly allocated to receive phosphate-buffered saline, LPS, or platonin (1000 μM). The cultures were harvested after exposing them to LPS for 18 h or a comparable duration in those groups without LPS. NO production, L-arginine transport, and expression of the relevant enzymes were then evaluated. Results: Platonin significantly attenuated LPS-induced up-regulation of iNOS expression and NO production in stimulated murine macrophages in a dose-dependent manner. Platonin also significantly inhibited up-regulation of CAT-2 and CAT-2B expression as well as L-arginine transport in LPS-stimulated murine macrophages in a dose-dependent manner. In contrast, CAT-2 A expression in murine macrophages was not affected by LPS and/or platonin. Conclusions: Platonin attenuates NO production and L-arginine transport in LPS-stimulated murine macrophages possibly through inhibiting iNOS, CAT-2, and CAT-2B expression.

AB - Background: Platonin, a cyanine photosensitizing dye, is a potent immunomodulator that suppresses acute inflammation. Platonin not only inhibits interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α production but also improves circulatory failure in septic rats. In addition, platonin reduces plasma nitric oxide (NO) formation during sepsis. However, the effects of platonin on inducible NO synthase (iNOS) and cationic amino-acid transporter (including CAT-2, CAT-2 A, and CAT-2B) expressions during sepsis remain uninvestigated. Methods: Five groups of confluent murine macrophages (RAW264.7 cells) were randomly allocated to receive a 1-h pretreatment of one of five doses of platonin (0.1 μM, 1 μM, 10 μM, 100 μM, or 1000 μM) followed by lipopolysaccharide (LPS; 100 ng ml-1). For negative, positive, and platonin control, three other groups of cell cultures were randomly allocated to receive phosphate-buffered saline, LPS, or platonin (1000 μM). The cultures were harvested after exposing them to LPS for 18 h or a comparable duration in those groups without LPS. NO production, L-arginine transport, and expression of the relevant enzymes were then evaluated. Results: Platonin significantly attenuated LPS-induced up-regulation of iNOS expression and NO production in stimulated murine macrophages in a dose-dependent manner. Platonin also significantly inhibited up-regulation of CAT-2 and CAT-2B expression as well as L-arginine transport in LPS-stimulated murine macrophages in a dose-dependent manner. In contrast, CAT-2 A expression in murine macrophages was not affected by LPS and/or platonin. Conclusions: Platonin attenuates NO production and L-arginine transport in LPS-stimulated murine macrophages possibly through inhibiting iNOS, CAT-2, and CAT-2B expression.

KW - CAT-2

KW - iNOS

KW - L-arginine

KW - LPS

KW - Macrophages

KW - Nitric oxide

KW - Platonin

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