Platonin, a cyanine photosensitizing dye, ameliorates inflammatory responses in vascular smooth muscle cells by modulating inflammatory transcription factors

Chih Wei Chiu, Chih Hao Yang, Jie Heng Tsai, Cheng Ying Hsieh, Shih Yi Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Inflammation of the arterial wall is critical to atherosclerosis pathogenesis. The switch of vascular smooth muscle cells (VSMCs) to macrophage-like cells is essential in the exacerbation of vascular inflammation. Platonin, a cyanine photosensitizing dye, exhibits protective effects in sepsis, trauma, and acute ischemic stroke through its anti-inflammatory capacity in macrophages. The present study investigated the effects and underlying mechanisms of platonin in inflammatory VSMCs. Pretreatment with platonin suppressed the expression of inducible nitric oxide synthetase and mature interleukin-1β but not that of monocyte chemoattractant protein-1 (MCP-1) in VSMCs stimulated by a combination of lipopolysaccharide and interferon-γ (LPS/IFN-γ). Furthermore, platonin inhibited LPS/IFN-γ-induced Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation though the direct reduction of p65Ser536 phosphorylation but not the restoration of Inhibitor of nuclear factor kappa B (IκBα) degradation in VSMCs. However, platonin inhibited Oxidized low-density lipoprotein (ox-LDL)-induced MCP-1 production, possibly through the at-tenuation of Activator protein 1 (AP-1) binding activity and C-Jun N-terminal kinases12(JNK1/2) phosphorylation. Platonin also lowered lipid drop accumulation in VSMCs in Oil red O staining assay. The results collectively indicated that platonin has a vascular protective property with potent anti-inflammatory effects in VSMCs. In conclusion, platonin should be a potential for treating vascular inflammatory diseases such as atherosclerosis.

Original languageEnglish
Article number1130
Pages (from-to)1-13
Number of pages13
JournalApplied Sciences (Switzerland)
Volume11
Issue number3
DOIs
Publication statusPublished - Feb 1 2021

Keywords

  • AP-1
  • Inflammation
  • NF-κB
  • Platonin
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

Fingerprint

Dive into the research topics of 'Platonin, a cyanine photosensitizing dye, ameliorates inflammatory responses in vascular smooth muscle cells by modulating inflammatory transcription factors'. Together they form a unique fingerprint.

Cite this