Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

Junko Nakao, Toshiro Ooyama, Wen Chang Chang, Sei itsu Murota, Hajime Orimo

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalAtherosclerosis
Volume43
Issue number2-3
DOIs
Publication statusPublished - 1982
Externally publishedYes

Keywords

  • 12-l-Hydroxy-5,8,10,14-eicosatetraenoic acid
  • Atherosclerosis
  • Chemokinesis
  • Platelet
  • Smooth muscle cell migration

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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