Platelets enhance biofilm formation and resistance of endocarditis-inducing streptococci on the injured heart valve

Chiau Jing Jung, Chiou Yueh Yeh, Chia Tung Shun, Ron Bin Hsu, Hung Wei Cheng, Chi Shuan Lin, Jean San Chia

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Infective endocarditis is a typical biofilm-associated infectious disease frequently caused by commensal streptococci, but the contribution of host factors in biofilm formation is unclear. We found that platelets are essential for in vitro biofilm formation by Streptococcus mutans or Streptococcus gordonii grown in human plasma. The biofilms were composed of bacterial floes embedded with platelet aggregates in layers, and a similar architecture was also detected in situ on the injured valves of a rat model of experimental endocarditis. Similar to planktonic cells, the streptococci in biofilms were also able to induce platelet aggregation, which facilitates multilayer biofilm formation. Entrapping of platelets directly enhances the resistance of streptococcal biofilms to clindamycin. Prophylactic antibiotics or aspirin can reduce but not prevent or abolish biofilm formation on injured heart valves. Therefore, the platelet is a host factor for commensal streptococci in the circulation to consolidate biofilm formation and protect bacteria against antibiotics.

Original languageEnglish
Pages (from-to)1066-1075
Number of pages10
JournalJournal of Infectious Diseases
Volume205
Issue number7
DOIs
Publication statusPublished - Apr 1 2012
Externally publishedYes

Fingerprint

Heart Valves
Biofilms
Endocarditis
Streptococcus
Blood Platelets
Streptococcus gordonii
Anti-Bacterial Agents
Streptococcus mutans
Clindamycin
Platelet Aggregation
Aspirin
Communicable Diseases
Theoretical Models
Bacteria

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Platelets enhance biofilm formation and resistance of endocarditis-inducing streptococci on the injured heart valve. / Jung, Chiau Jing; Yeh, Chiou Yueh; Shun, Chia Tung; Hsu, Ron Bin; Cheng, Hung Wei; Lin, Chi Shuan; Chia, Jean San.

In: Journal of Infectious Diseases, Vol. 205, No. 7, 01.04.2012, p. 1066-1075.

Research output: Contribution to journalArticle

Jung, Chiau Jing ; Yeh, Chiou Yueh ; Shun, Chia Tung ; Hsu, Ron Bin ; Cheng, Hung Wei ; Lin, Chi Shuan ; Chia, Jean San. / Platelets enhance biofilm formation and resistance of endocarditis-inducing streptococci on the injured heart valve. In: Journal of Infectious Diseases. 2012 ; Vol. 205, No. 7. pp. 1066-1075.
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