Platelet microparticles: Detection and assessment of their paradoxical functional roles in disease and regenerative medicine

Thierry Burnouf, Hadi Alphonse Goubran, Ming Li Chou, David Devos, Mirjana Radosevic

Research output: Contribution to journalReview article

82 Citations (Scopus)

Abstract

There is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). PMPs are 0.1-1-μm fragments shed from plasma membranes of platelets that are undergoing activation, stress, or apoptosis. They have a phospholipid-based structure and express functional receptors from platelet membranes. As they are the most abundant microparticles in the blood and they express the procoagulant phosphatidylserine, PMPs likely complement, if not amplify, the functions of platelets in hemostasis, thrombosis, cancer, and inflammation, but also act as promoters of tissue regeneration. Their size and structure make them instrumental in platelet-cell communications as a delivery tool of platelet-borne bioactive molecules including growth factors, other signaling molecules and micro (mi)RNA. PMPs can therefore be a pathophysiological threat or benefit to the cellular environment when interacting with the blood vasculature. There is also increasing evidence that PMP generation is triggered during blood collection, separation into components, and storage, a phenomenon potentially leading to thrombotic and inflammatory side effects in transfused patients. Evaluating PMPs requires strict pre-analytical and analytical procedures to avoid artifactual generation and ensure accurate assessment of the number, size repartitioning, and functional properties. This review describes the physical and functional methods developed for analyzing and quantifying PMPs. It then presents the functional roles of PMPs as markers or triggers of diseases like thrombosis, atherosclerosis, and cancer, and discusses the possible detrimental immunological impact of their generation in blood components. Finally we review the potential function of PMPs in tissue regeneration and the prospects for their use in therapeutic strategies for human health.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalBlood Reviews
Volume28
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Regenerative Medicine
Blood Platelets
Regeneration
Thrombosis
Phosphatidylserines
Platelet Activation
Therapeutic Uses
Hemostasis
MicroRNAs
Cell Communication
Neoplasms
Phospholipids
Atherosclerosis
Intercellular Signaling Peptides and Proteins

Keywords

  • Blood transfusion
  • Detection
  • Microparticles
  • Pathology
  • Platelets
  • Regenerative medicine

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Medicine(all)

Cite this

Platelet microparticles : Detection and assessment of their paradoxical functional roles in disease and regenerative medicine. / Burnouf, Thierry; Goubran, Hadi Alphonse; Chou, Ming Li; Devos, David; Radosevic, Mirjana.

In: Blood Reviews, Vol. 28, No. 4, 2014, p. 155-166.

Research output: Contribution to journalReview article

Burnouf, Thierry ; Goubran, Hadi Alphonse ; Chou, Ming Li ; Devos, David ; Radosevic, Mirjana. / Platelet microparticles : Detection and assessment of their paradoxical functional roles in disease and regenerative medicine. In: Blood Reviews. 2014 ; Vol. 28, No. 4. pp. 155-166.
@article{30280f2ae180470d953068745118cfe7,
title = "Platelet microparticles: Detection and assessment of their paradoxical functional roles in disease and regenerative medicine",
abstract = "There is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). PMPs are 0.1-1-μm fragments shed from plasma membranes of platelets that are undergoing activation, stress, or apoptosis. They have a phospholipid-based structure and express functional receptors from platelet membranes. As they are the most abundant microparticles in the blood and they express the procoagulant phosphatidylserine, PMPs likely complement, if not amplify, the functions of platelets in hemostasis, thrombosis, cancer, and inflammation, but also act as promoters of tissue regeneration. Their size and structure make them instrumental in platelet-cell communications as a delivery tool of platelet-borne bioactive molecules including growth factors, other signaling molecules and micro (mi)RNA. PMPs can therefore be a pathophysiological threat or benefit to the cellular environment when interacting with the blood vasculature. There is also increasing evidence that PMP generation is triggered during blood collection, separation into components, and storage, a phenomenon potentially leading to thrombotic and inflammatory side effects in transfused patients. Evaluating PMPs requires strict pre-analytical and analytical procedures to avoid artifactual generation and ensure accurate assessment of the number, size repartitioning, and functional properties. This review describes the physical and functional methods developed for analyzing and quantifying PMPs. It then presents the functional roles of PMPs as markers or triggers of diseases like thrombosis, atherosclerosis, and cancer, and discusses the possible detrimental immunological impact of their generation in blood components. Finally we review the potential function of PMPs in tissue regeneration and the prospects for their use in therapeutic strategies for human health.",
keywords = "Blood transfusion, Detection, Microparticles, Pathology, Platelets, Regenerative medicine",
author = "Thierry Burnouf and Goubran, {Hadi Alphonse} and Chou, {Ming Li} and David Devos and Mirjana Radosevic",
year = "2014",
doi = "10.1016/j.blre.2014.04.002",
language = "English",
volume = "28",
pages = "155--166",
journal = "Blood Reviews",
issn = "0268-960X",
publisher = "Churchill Livingstone",
number = "4",

}

TY - JOUR

T1 - Platelet microparticles

T2 - Detection and assessment of their paradoxical functional roles in disease and regenerative medicine

AU - Burnouf, Thierry

AU - Goubran, Hadi Alphonse

AU - Chou, Ming Li

AU - Devos, David

AU - Radosevic, Mirjana

PY - 2014

Y1 - 2014

N2 - There is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). PMPs are 0.1-1-μm fragments shed from plasma membranes of platelets that are undergoing activation, stress, or apoptosis. They have a phospholipid-based structure and express functional receptors from platelet membranes. As they are the most abundant microparticles in the blood and they express the procoagulant phosphatidylserine, PMPs likely complement, if not amplify, the functions of platelets in hemostasis, thrombosis, cancer, and inflammation, but also act as promoters of tissue regeneration. Their size and structure make them instrumental in platelet-cell communications as a delivery tool of platelet-borne bioactive molecules including growth factors, other signaling molecules and micro (mi)RNA. PMPs can therefore be a pathophysiological threat or benefit to the cellular environment when interacting with the blood vasculature. There is also increasing evidence that PMP generation is triggered during blood collection, separation into components, and storage, a phenomenon potentially leading to thrombotic and inflammatory side effects in transfused patients. Evaluating PMPs requires strict pre-analytical and analytical procedures to avoid artifactual generation and ensure accurate assessment of the number, size repartitioning, and functional properties. This review describes the physical and functional methods developed for analyzing and quantifying PMPs. It then presents the functional roles of PMPs as markers or triggers of diseases like thrombosis, atherosclerosis, and cancer, and discusses the possible detrimental immunological impact of their generation in blood components. Finally we review the potential function of PMPs in tissue regeneration and the prospects for their use in therapeutic strategies for human health.

AB - There is increasing research on and clinical interest in the physiological role played by platelet microparticles (PMPs). PMPs are 0.1-1-μm fragments shed from plasma membranes of platelets that are undergoing activation, stress, or apoptosis. They have a phospholipid-based structure and express functional receptors from platelet membranes. As they are the most abundant microparticles in the blood and they express the procoagulant phosphatidylserine, PMPs likely complement, if not amplify, the functions of platelets in hemostasis, thrombosis, cancer, and inflammation, but also act as promoters of tissue regeneration. Their size and structure make them instrumental in platelet-cell communications as a delivery tool of platelet-borne bioactive molecules including growth factors, other signaling molecules and micro (mi)RNA. PMPs can therefore be a pathophysiological threat or benefit to the cellular environment when interacting with the blood vasculature. There is also increasing evidence that PMP generation is triggered during blood collection, separation into components, and storage, a phenomenon potentially leading to thrombotic and inflammatory side effects in transfused patients. Evaluating PMPs requires strict pre-analytical and analytical procedures to avoid artifactual generation and ensure accurate assessment of the number, size repartitioning, and functional properties. This review describes the physical and functional methods developed for analyzing and quantifying PMPs. It then presents the functional roles of PMPs as markers or triggers of diseases like thrombosis, atherosclerosis, and cancer, and discusses the possible detrimental immunological impact of their generation in blood components. Finally we review the potential function of PMPs in tissue regeneration and the prospects for their use in therapeutic strategies for human health.

KW - Blood transfusion

KW - Detection

KW - Microparticles

KW - Pathology

KW - Platelets

KW - Regenerative medicine

UR - http://www.scopus.com/inward/record.url?scp=84903150116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903150116&partnerID=8YFLogxK

U2 - 10.1016/j.blre.2014.04.002

DO - 10.1016/j.blre.2014.04.002

M3 - Review article

C2 - 24826991

AN - SCOPUS:84903150116

VL - 28

SP - 155

EP - 166

JO - Blood Reviews

JF - Blood Reviews

SN - 0268-960X

IS - 4

ER -