Platelet concentrate supernatants alter endothelial cell mRNA and protein expression patterns as a function of storage length

Caroline Sut, Hind Hamzeh-Cognasse, Charles Antoine Arthaud, Marie Ange Eyraud, Kamel Chettab, Charles Dumontet, Sandrine Laradi, Thierry Burnouf, Olivier Garraud, Fabrice Cognasse

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BACKGROUND: Platelet transfusions are safe but can nevertheless cause serious adverse reactions (SARs). This study investigated the effects of platelet biological response modifiers (BRMs) that accumulate during storage and are commonly associated with transfusion adverse reactions. STUDY DESIGN AND METHODS: Endothelial cells (ECs), that is, EA.hy926, were exposed in vitro to supernatants of platelet components (PCs) that had been either implicated or not in SARs. The EC Biology RT2 Profiler PCR Array was used at the same time to study 84 genes related to functions of ECs. Soluble cytokines and surface expression of EC markers were determined by Luminex/enzyme-linked immunosorbent assay technology and flow cytometry, respectively. Apoptosis and scratch wound assays were performed using IncuCyte technology. RESULTS: In vitro exposure of EA.hy926 monolayers with Day 0, 1-2, and 3-4 stored PC supernatants resulted in decreases in surface expression of markers of ECs. There was differential production of soluble BRMs in the tested cell line. Exposure to the supernatants of PCs that had been implicated in SARs showed a significant difference in the expression of the EC surface markers. EC mediators also responded differently when exposed to PC supernatants of different storage times and PCs involved in SARs. CONCLUSION: PC supernatants collected at Day 1-2 activate fewer cell lines of ECs compared with supernatants collected at Day 3-4. Moreover, PC supernatants involved in SARs appear to alter EC activation compared with the control and storage length.

Original languageEnglish
JournalTransfusion
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Blood Platelets
Endothelial Cells
Messenger RNA
Proteins
Immunologic Factors
Technology
Cell Line
Platelet Transfusion
Cell Biology
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Apoptosis
Cytokines
Polymerase Chain Reaction
Wounds and Injuries
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Sut, C., Hamzeh-Cognasse, H., Arthaud, C. A., Eyraud, M. A., Chettab, K., Dumontet, C., ... Cognasse, F. (Accepted/In press). Platelet concentrate supernatants alter endothelial cell mRNA and protein expression patterns as a function of storage length. Transfusion. https://doi.org/10.1111/trf.14973

Platelet concentrate supernatants alter endothelial cell mRNA and protein expression patterns as a function of storage length. / Sut, Caroline; Hamzeh-Cognasse, Hind; Arthaud, Charles Antoine; Eyraud, Marie Ange; Chettab, Kamel; Dumontet, Charles; Laradi, Sandrine; Burnouf, Thierry; Garraud, Olivier; Cognasse, Fabrice.

In: Transfusion, 01.01.2018.

Research output: Contribution to journalArticle

Sut, C, Hamzeh-Cognasse, H, Arthaud, CA, Eyraud, MA, Chettab, K, Dumontet, C, Laradi, S, Burnouf, T, Garraud, O & Cognasse, F 2018, 'Platelet concentrate supernatants alter endothelial cell mRNA and protein expression patterns as a function of storage length', Transfusion. https://doi.org/10.1111/trf.14973
Sut, Caroline ; Hamzeh-Cognasse, Hind ; Arthaud, Charles Antoine ; Eyraud, Marie Ange ; Chettab, Kamel ; Dumontet, Charles ; Laradi, Sandrine ; Burnouf, Thierry ; Garraud, Olivier ; Cognasse, Fabrice. / Platelet concentrate supernatants alter endothelial cell mRNA and protein expression patterns as a function of storage length. In: Transfusion. 2018.
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AU - Dumontet, Charles

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