Platelet adhesion following the administration of tissue-type plasminogen activator and its reversal by vitamin E in rats

Chauying J. Jen, Huei Ping Dong, Hsiun Ing Chen, Lih Yuh C Wing, Ming Jer Tang, Wen Chang Chang, Ming T. Lin, Guey Yueh Shi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Thrombolytic therapy is known to induce platelet-related side effects. We used a parallel-plate flow chamber, which was connected to the femoral artery of the rat, to measure platelet adhesion ex vivo. A collagen-coated arterioarterial shunt between two carotid arteries was used to measure shunt patency duration as an index of antithrombotic efficacy. Tissue-type plasminogen activator (t-PA), vitamin E, and the combination of these two were intravenously administered for 60 min. Measurements were performed before drug administration, and at 30, 60, 120 min after the initiation of drug infusion. Our results indicated that (1) treatments with t-PA or t-PA/vitamin E prolonged the time to shunt occlusion at 30 and 60 min; (2) t-PA enhanced platelet adhesion at 60 and 120 min; (3) vitamin E tended to reduce platelet adhesion; (4) t-PA/vitamin E reduced the t-PA-enhanced platelet adhesion; (5) at the high-density area of platelet adhesion under t-PA treatment, the adherent platelets demonstrated severe morphological changes which could be blocked by vitamin E. These data suggest that t-PA may enhance platelet adhesion in rats and that this adverse effect can be suppressed by co-administration of vitamin E.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalThrombosis and Haemostasis
Volume75
Issue number1
Publication statusPublished - Jan 1996
Externally publishedYes

Fingerprint

Tissue Plasminogen Activator
Plasminogen Activators
Vitamin E
Blood Platelets
Thrombolytic Therapy
Femoral Artery
Carotid Arteries
Pharmaceutical Preparations
Collagen
Therapeutics

ASJC Scopus subject areas

  • Hematology

Cite this

Jen, C. J., Dong, H. P., Chen, H. I., Wing, L. Y. C., Tang, M. J., Chang, W. C., ... Shi, G. Y. (1996). Platelet adhesion following the administration of tissue-type plasminogen activator and its reversal by vitamin E in rats. Thrombosis and Haemostasis, 75(1), 101-106.

Platelet adhesion following the administration of tissue-type plasminogen activator and its reversal by vitamin E in rats. / Jen, Chauying J.; Dong, Huei Ping; Chen, Hsiun Ing; Wing, Lih Yuh C; Tang, Ming Jer; Chang, Wen Chang; Lin, Ming T.; Shi, Guey Yueh.

In: Thrombosis and Haemostasis, Vol. 75, No. 1, 01.1996, p. 101-106.

Research output: Contribution to journalArticle

Jen, CJ, Dong, HP, Chen, HI, Wing, LYC, Tang, MJ, Chang, WC, Lin, MT & Shi, GY 1996, 'Platelet adhesion following the administration of tissue-type plasminogen activator and its reversal by vitamin E in rats', Thrombosis and Haemostasis, vol. 75, no. 1, pp. 101-106.
Jen, Chauying J. ; Dong, Huei Ping ; Chen, Hsiun Ing ; Wing, Lih Yuh C ; Tang, Ming Jer ; Chang, Wen Chang ; Lin, Ming T. ; Shi, Guey Yueh. / Platelet adhesion following the administration of tissue-type plasminogen activator and its reversal by vitamin E in rats. In: Thrombosis and Haemostasis. 1996 ; Vol. 75, No. 1. pp. 101-106.
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