Placenta-derived multipotent cells exhibit immunosuppressive properties that are enhanced in the presence of interferon-γ

Chun Jung Chang, Men Luh Yen, Yao Chang Chen, Chih Cheng Chien, Hsing I. Huang, Chyi Huey Bai, B. Linju Yen

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Several types of nonhematopoietic stem cells, including bone marrow mesenchymal stem cells (BMMSCs) and embryonic stem cells, have been shown to have immunosuppressive properties. We show that human placenta-derived multipotent cells (PDMCs), which are isolated from a source without ethical concern and harbor multilineage differentiation potential, have strong immunosuppressive properties. PDMCs suppress both mitogen-induced and allogeneic lymphocyte proliferation in both CD4 and CD8 populations. The immunosuppression seen with PDMCs was significantly stronger than that with BMMSCs. Both PDMCs and BMMSCs express indoleamine 2,3-dioxygenase, but only PDMCs are positive for intracellular human leukocyte antigen-G (HLA). Mechanistically, suppression of lymphocyte reactivity by PDMCs is not due to cell death but to decreased cell proliferation and increased numbers of regulatory T cells. Addition of neutralizing antibodies to interleukin-10 and transforming growth factor (TGF)-β partially restored lymphocyte proliferation. Unlike BMMSCs, PDMCs treated with interferon-γ for 3 days only very minimally upregulated HLA-DR. On the contrary, PD-L1, a cell surface marker that plays an inhibitory role in T-cell activation, was upregulated and TGF-β expression was seen. The immunosuppressive properties of PDMCs, along with their multilineage differentiation potential, ease of accessibility, and abundant cell numbers, may render these cells as good potential sources for future therapeutic applications.

Original languageEnglish
Pages (from-to)2466-2477
Number of pages12
JournalStem Cells
Volume24
Issue number11
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Immunosuppressive Agents
Interferons
Placenta
Mesenchymal Stromal Cells
Bone Marrow
Transforming Growth Factors
Lymphocytes
HLA Antigens
Indoleamine-Pyrrole 2,3,-Dioxygenase
Regulatory T-Lymphocytes
Embryonic Stem Cells
Neutralizing Antibodies
Mitogens
Bone Marrow Cells
Interleukin-10
Immunosuppression
Cell Death
Stem Cells
Cell Count
Cell Proliferation

Keywords

  • Human leukocyte antigen, class I, G
  • Immunosuppression
  • Indoleamine 2,3-dioxygenase
  • Mesenchymal stem cell
  • Mixed lymphocyte culture interferon-γ
  • Multilineage differentiation
  • Placenta

ASJC Scopus subject areas

  • Cell Biology

Cite this

Placenta-derived multipotent cells exhibit immunosuppressive properties that are enhanced in the presence of interferon-γ. / Chang, Chun Jung; Yen, Men Luh; Chen, Yao Chang; Chien, Chih Cheng; Huang, Hsing I.; Bai, Chyi Huey; Yen, B. Linju.

In: Stem Cells, Vol. 24, No. 11, 2006, p. 2466-2477.

Research output: Contribution to journalArticle

Chang, Chun Jung ; Yen, Men Luh ; Chen, Yao Chang ; Chien, Chih Cheng ; Huang, Hsing I. ; Bai, Chyi Huey ; Yen, B. Linju. / Placenta-derived multipotent cells exhibit immunosuppressive properties that are enhanced in the presence of interferon-γ. In: Stem Cells. 2006 ; Vol. 24, No. 11. pp. 2466-2477.
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