PKCβI mediates the inhibition of P2Y receptor-induced inositol phosphate formation in endothelial cells

Bing C. Chen, Wan W. Lin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

1. Bovine pulmonary artery endothelium (CPAE) expresses phospholipase C (PLC)-linked P2Y1 and P2Y2 receptors, for them 2-methylthio-ATP (2MeSATP) and UTP are respective agonists. Here, we have investigated the particular protein kinase C (PKC) isoform(s) responsible for the inhibition of P2Y1 and P2Y2 receptor-evoked inositol phosphate (IP) formation by phorbol 12-myristate 13-acetate (PMA). 2. Although short-term (20 min) pretreatment of cells with PMA attenuated 2MeSATP- and UTP-induced phosphoinositide (PI) breakdown, this inhibition was lost after 15 h. Preincubation with PMA for 24 h, on the contrary, potentiated 2MeSATP and UTP responses. The IP formation stimulated by NaF was unaltered by PMA pretreatment. 3. Western blot analysis showed that treatment of CPAE with PMA resulted in a rapid translocation of PKC isoform βI, ε and μ, but not λ, from the cytosol to the membrane fraction. 4. Pretreatment of the selective PKC inhibitor Ro 31-8220 attenuated the inhibitory effect of PMA on IP formation. Go 6976 (an inhibitor of conventional PKCα, β and γ) and LY 379196 (a selective PKCβ inhibitor) also dose-dependently inhibited the PMA-mediated desensitization. 5. Transfection of PKCβ-specific antisense oligonucleotide reduced PKCβI protein level and inhibited PMA-mediated PI reduction, 6. RT-PCR analysis showed that PMA treatment for 4-24 h up-regulated P2Y1 and P2Y2 receptors at the mRNA levels. 7. These results suggest that PKCβI may exert a negative feedback regulation on endothelial P2Y1 and P2Y1 receptor-mediated PI turnover. The down-regulation of PKCβI and enhanced P2Y receptor expression together might contribute to the late PI enhancing effect of PMA.

Original languageEnglish
Pages (from-to)1908-1914
Number of pages7
JournalBritish Journal of Pharmacology
Volume127
Issue number8
DOIs
Publication statusPublished - 1999
Externally publishedYes

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Inositol Phosphates
Acetates
Endothelial Cells
Purinergic P2Y1 Receptors
Protein Kinase C
Purinergic P2Y2 Receptors
Phosphatidylinositols
Uridine Triphosphate
Protein C Inhibitor
Protein Kinase Inhibitors
5,21 - 12,17-dimetheneo-18H-dibenzo(i,o)pyrrolo(3,4-1)(1,8)diazacyclohexandecine-18,10(19H)dione,8((dimethylamino)methyl)-6,7,8,9,10,11-hexahydro,monomethanesulfonate
Protein Isoforms
phorbol-12-myristate
Antisense Oligonucleotides
Type C Phospholipases
Cytosol
Pulmonary Artery
Endothelium
Transfection
Down-Regulation

Keywords

  • 2MeSATP
  • Endothelium
  • P2 receptors
  • PI turnover
  • PKCβI
  • Receptor up-regulation
  • UTP

ASJC Scopus subject areas

  • Pharmacology

Cite this

PKCβI mediates the inhibition of P2Y receptor-induced inositol phosphate formation in endothelial cells. / Chen, Bing C.; Lin, Wan W.

In: British Journal of Pharmacology, Vol. 127, No. 8, 1999, p. 1908-1914.

Research output: Contribution to journalArticle

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abstract = "1. Bovine pulmonary artery endothelium (CPAE) expresses phospholipase C (PLC)-linked P2Y1 and P2Y2 receptors, for them 2-methylthio-ATP (2MeSATP) and UTP are respective agonists. Here, we have investigated the particular protein kinase C (PKC) isoform(s) responsible for the inhibition of P2Y1 and P2Y2 receptor-evoked inositol phosphate (IP) formation by phorbol 12-myristate 13-acetate (PMA). 2. Although short-term (20 min) pretreatment of cells with PMA attenuated 2MeSATP- and UTP-induced phosphoinositide (PI) breakdown, this inhibition was lost after 15 h. Preincubation with PMA for 24 h, on the contrary, potentiated 2MeSATP and UTP responses. The IP formation stimulated by NaF was unaltered by PMA pretreatment. 3. Western blot analysis showed that treatment of CPAE with PMA resulted in a rapid translocation of PKC isoform βI, ε and μ, but not λ, from the cytosol to the membrane fraction. 4. Pretreatment of the selective PKC inhibitor Ro 31-8220 attenuated the inhibitory effect of PMA on IP formation. Go 6976 (an inhibitor of conventional PKCα, β and γ) and LY 379196 (a selective PKCβ inhibitor) also dose-dependently inhibited the PMA-mediated desensitization. 5. Transfection of PKCβ-specific antisense oligonucleotide reduced PKCβI protein level and inhibited PMA-mediated PI reduction, 6. RT-PCR analysis showed that PMA treatment for 4-24 h up-regulated P2Y1 and P2Y2 receptors at the mRNA levels. 7. These results suggest that PKCβI may exert a negative feedback regulation on endothelial P2Y1 and P2Y1 receptor-mediated PI turnover. The down-regulation of PKCβI and enhanced P2Y receptor expression together might contribute to the late PI enhancing effect of PMA.",
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AB - 1. Bovine pulmonary artery endothelium (CPAE) expresses phospholipase C (PLC)-linked P2Y1 and P2Y2 receptors, for them 2-methylthio-ATP (2MeSATP) and UTP are respective agonists. Here, we have investigated the particular protein kinase C (PKC) isoform(s) responsible for the inhibition of P2Y1 and P2Y2 receptor-evoked inositol phosphate (IP) formation by phorbol 12-myristate 13-acetate (PMA). 2. Although short-term (20 min) pretreatment of cells with PMA attenuated 2MeSATP- and UTP-induced phosphoinositide (PI) breakdown, this inhibition was lost after 15 h. Preincubation with PMA for 24 h, on the contrary, potentiated 2MeSATP and UTP responses. The IP formation stimulated by NaF was unaltered by PMA pretreatment. 3. Western blot analysis showed that treatment of CPAE with PMA resulted in a rapid translocation of PKC isoform βI, ε and μ, but not λ, from the cytosol to the membrane fraction. 4. Pretreatment of the selective PKC inhibitor Ro 31-8220 attenuated the inhibitory effect of PMA on IP formation. Go 6976 (an inhibitor of conventional PKCα, β and γ) and LY 379196 (a selective PKCβ inhibitor) also dose-dependently inhibited the PMA-mediated desensitization. 5. Transfection of PKCβ-specific antisense oligonucleotide reduced PKCβI protein level and inhibited PMA-mediated PI reduction, 6. RT-PCR analysis showed that PMA treatment for 4-24 h up-regulated P2Y1 and P2Y2 receptors at the mRNA levels. 7. These results suggest that PKCβI may exert a negative feedback regulation on endothelial P2Y1 and P2Y1 receptor-mediated PI turnover. The down-regulation of PKCβI and enhanced P2Y receptor expression together might contribute to the late PI enhancing effect of PMA.

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KW - PKCβI

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