Piscidin-1, an antimicrobial peptide from fish (Hybrid Striped Bass Morone saxatilis x M. chrysops), induces apoptotic and necrotic activity in HT1080 cells

Heng Ju Lin, Tsui Chin Huang, Sasikala Muthusamy, Jheng Fong Lee, Yeh Fang Duann, Cheng Hui Lin

Research output: Contribution to journalArticle

30 Citations (Scopus)


Piscidin-1, a 22-residue cationic peptide isolated from mast cells of a hybrid striped bass, has potent antimicrobial activities against both gram-positive and-negative bacteria. To date, there is no report of its antitumor activity on any tumor cell lines. In this study, we examined the antitumor activity of a synthetic piscidin-1 peptide against several human cancer cell lines using an MTS assay and soft-agar colony-formation assay. We found that a low dose of piscidin induces both apoptosis and necrosis in HT1080 cells, as shown by annexin-V/propidium iodide and acridine orange/ethidium bromide staining, and triggers a necrotic cell death pathway in a short period with high-dose treatment. The destruction of cell membranes by piscidin-1 was demonstrated by transmission electron microscopy. Furthermore, piscidin-1 also inhibits the migration of HT1080 cells in a dose-dependent manner. This study provides the first evidence of the anticancer activity of the antimicrobial peptide, piscidin-1, with potential implications for the treatment of cancer.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalZoological Science
Issue number5
Publication statusPublished - May 2012
Externally publishedYes



  • Antimicrobial peptides (AMPs)
  • Apoptotic activity
  • Cytotoxicity
  • Necrotic activity
  • Piscidin-1

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this