Pipoxolan inhibits proliferation of HL-60 human leukaemia cancer cells by arresting the cell cycle at the G0/G1 phase

Ming Jyh Sheu, Pei Yu Chou, Chin Shiu Huang, I. Chun Tsai, Yi Chung Chien, Sung Yuan Lin, Huei Yann Tsai, Hsu Chen Cheng, Chieh His Wu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. The aim of the present study was to investigate the molecular mechanisms by which pipoxolan exerts its inhibitory effects and apoptotic activity in human leukaemia HL-60 cells. 2. The effects of pipoxolan on the proliferation of HL-60 cells and on the distribution of cells within different phases of the cell cycle were investigated indirectly using a Trypan blue assay and a flow cytometer, respectively. The effects of pipoxolan on the apoptosis of HL-60 cells was investigated using DNA fragmentation and flow cytometer. The expression of factors affecting the cell cycle and apoptosis, including p53, p21, Bax, Bcl2, cytochrome c, caspase 3 and caspase 9, was examined by western blotting. 3. At 6.25 μg/mL, pipoxolan significantly induced apoptosis in human leukaemia HL-60 cells after 24 h exposure. In addition, HL-60 cells were arrested in the G0/G1 phase via the induction of p53/p21 by pipoxolan. Apoptosis was associated with an increased Bax/Bcl-2 ratio, cytochrome c release, cleavage of procaspases-9 and -3 and hydrolysis of poly(ADP-ribose) polymerase. Intracellular reactive oxygen species (ROS) seem to play a key role in the pipoxolan-induced apoptosis, because high levels of ROS were produced early in the drug treatment. Apoptosis was significantly abrogated by the free radical scavenger N-acetylcysteine (NAC).

Original languageEnglish
Pages (from-to)605-612
Number of pages8
JournalClinical and Experimental Pharmacology and Physiology
Volume37
Issue number5-6
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Anti-proliferation
  • Apoptosis
  • Caspase 3
  • Pipoxolan
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Pharmacology
  • Medicine(all)

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