PIK3CA as an oncogene in cervical cancer

Yen Ying Ma, Sung Jen Wei, Yu Chen Lin, Jia Chyi Lung, Ting Chang Chang, Jacqueline Whang-Peng, Jacqueline M. Liu, Deng Mei Yang, Wen K. Yang, Chen Yang Shen

Research output: Contribution to journalArticle

360 Citations (Scopus)

Abstract

Amplification of chromosome arm 3q is the most consistent aberration in cervical cancer, and is implicated in the progression of dysplastic uterine cervical cells into invasive cancer. The present study employed the 'positional candidate gene' strategy to determine the contribution of PIK3CA, which is located in 3q26.3, in cervical tumorigenesis. PIK3CA is known to be involved in the PI 3-kinase/AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. The results of comparative genomic hybridization show that the 3q26.3 amplification was the most consistent chromosomal aberration in primary tissues of cervical carcinoma, and a positive correlation between an increased copy number of PIK3CA (detected by competitive PCR) and 3q26.3 amplification was found in tumor tissues and in cervical cancer cell lines. In cervical cancer cell lines harboring amplified PIK3CA, the expression of gene product (p110α) of PIK3CA was increased, and was subsequently associated with high kinase activity. In addition, transformation phenotypes in these lines, including increased cell growth and decreased apoptosis, were found to be significantly affected by the treatment of specific PI 3-kinase inhibitor, suggesting that increased expression of PIK3CA in cervical cancer may result in promoting cell proliferation and reducing apoptosis. These evidences support that PIK3CA is an oncogene in cervical cancer and PIK3CA amplification may be linked to cervical tumorigenesis.

Original languageEnglish
Pages (from-to)2739-2744
Number of pages6
JournalOncogene
Volume19
Issue number23
Publication statusPublished - May 25 2000
Externally publishedYes

Fingerprint

Oncogenes
Uterine Cervical Neoplasms
Apoptosis
Phosphatidylinositol 3-Kinases
Carcinogenesis
Cell Line
Comparative Genomic Hybridization
Growth
Chromosome Aberrations
Neoplasms
Phosphotransferases
Chromosomes
Cell Proliferation
Carcinoma
Phenotype
Gene Expression
Polymerase Chain Reaction
Genes

Keywords

  • 3q26.3
  • AKT
  • Cervical cancer
  • p110α
  • PI 3-kinase
  • PIK3CA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Ma, Y. Y., Wei, S. J., Lin, Y. C., Lung, J. C., Chang, T. C., Whang-Peng, J., ... Shen, C. Y. (2000). PIK3CA as an oncogene in cervical cancer. Oncogene, 19(23), 2739-2744.

PIK3CA as an oncogene in cervical cancer. / Ma, Yen Ying; Wei, Sung Jen; Lin, Yu Chen; Lung, Jia Chyi; Chang, Ting Chang; Whang-Peng, Jacqueline; Liu, Jacqueline M.; Yang, Deng Mei; Yang, Wen K.; Shen, Chen Yang.

In: Oncogene, Vol. 19, No. 23, 25.05.2000, p. 2739-2744.

Research output: Contribution to journalArticle

Ma, YY, Wei, SJ, Lin, YC, Lung, JC, Chang, TC, Whang-Peng, J, Liu, JM, Yang, DM, Yang, WK & Shen, CY 2000, 'PIK3CA as an oncogene in cervical cancer', Oncogene, vol. 19, no. 23, pp. 2739-2744.
Ma YY, Wei SJ, Lin YC, Lung JC, Chang TC, Whang-Peng J et al. PIK3CA as an oncogene in cervical cancer. Oncogene. 2000 May 25;19(23):2739-2744.
Ma, Yen Ying ; Wei, Sung Jen ; Lin, Yu Chen ; Lung, Jia Chyi ; Chang, Ting Chang ; Whang-Peng, Jacqueline ; Liu, Jacqueline M. ; Yang, Deng Mei ; Yang, Wen K. ; Shen, Chen Yang. / PIK3CA as an oncogene in cervical cancer. In: Oncogene. 2000 ; Vol. 19, No. 23. pp. 2739-2744.
@article{ebbf615e5faa4147806abc57398dd19c,
title = "PIK3CA as an oncogene in cervical cancer",
abstract = "Amplification of chromosome arm 3q is the most consistent aberration in cervical cancer, and is implicated in the progression of dysplastic uterine cervical cells into invasive cancer. The present study employed the 'positional candidate gene' strategy to determine the contribution of PIK3CA, which is located in 3q26.3, in cervical tumorigenesis. PIK3CA is known to be involved in the PI 3-kinase/AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. The results of comparative genomic hybridization show that the 3q26.3 amplification was the most consistent chromosomal aberration in primary tissues of cervical carcinoma, and a positive correlation between an increased copy number of PIK3CA (detected by competitive PCR) and 3q26.3 amplification was found in tumor tissues and in cervical cancer cell lines. In cervical cancer cell lines harboring amplified PIK3CA, the expression of gene product (p110α) of PIK3CA was increased, and was subsequently associated with high kinase activity. In addition, transformation phenotypes in these lines, including increased cell growth and decreased apoptosis, were found to be significantly affected by the treatment of specific PI 3-kinase inhibitor, suggesting that increased expression of PIK3CA in cervical cancer may result in promoting cell proliferation and reducing apoptosis. These evidences support that PIK3CA is an oncogene in cervical cancer and PIK3CA amplification may be linked to cervical tumorigenesis.",
keywords = "3q26.3, AKT, Cervical cancer, p110α, PI 3-kinase, PIK3CA",
author = "Ma, {Yen Ying} and Wei, {Sung Jen} and Lin, {Yu Chen} and Lung, {Jia Chyi} and Chang, {Ting Chang} and Jacqueline Whang-Peng and Liu, {Jacqueline M.} and Yang, {Deng Mei} and Yang, {Wen K.} and Shen, {Chen Yang}",
year = "2000",
month = "5",
day = "25",
language = "English",
volume = "19",
pages = "2739--2744",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "23",

}

TY - JOUR

T1 - PIK3CA as an oncogene in cervical cancer

AU - Ma, Yen Ying

AU - Wei, Sung Jen

AU - Lin, Yu Chen

AU - Lung, Jia Chyi

AU - Chang, Ting Chang

AU - Whang-Peng, Jacqueline

AU - Liu, Jacqueline M.

AU - Yang, Deng Mei

AU - Yang, Wen K.

AU - Shen, Chen Yang

PY - 2000/5/25

Y1 - 2000/5/25

N2 - Amplification of chromosome arm 3q is the most consistent aberration in cervical cancer, and is implicated in the progression of dysplastic uterine cervical cells into invasive cancer. The present study employed the 'positional candidate gene' strategy to determine the contribution of PIK3CA, which is located in 3q26.3, in cervical tumorigenesis. PIK3CA is known to be involved in the PI 3-kinase/AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. The results of comparative genomic hybridization show that the 3q26.3 amplification was the most consistent chromosomal aberration in primary tissues of cervical carcinoma, and a positive correlation between an increased copy number of PIK3CA (detected by competitive PCR) and 3q26.3 amplification was found in tumor tissues and in cervical cancer cell lines. In cervical cancer cell lines harboring amplified PIK3CA, the expression of gene product (p110α) of PIK3CA was increased, and was subsequently associated with high kinase activity. In addition, transformation phenotypes in these lines, including increased cell growth and decreased apoptosis, were found to be significantly affected by the treatment of specific PI 3-kinase inhibitor, suggesting that increased expression of PIK3CA in cervical cancer may result in promoting cell proliferation and reducing apoptosis. These evidences support that PIK3CA is an oncogene in cervical cancer and PIK3CA amplification may be linked to cervical tumorigenesis.

AB - Amplification of chromosome arm 3q is the most consistent aberration in cervical cancer, and is implicated in the progression of dysplastic uterine cervical cells into invasive cancer. The present study employed the 'positional candidate gene' strategy to determine the contribution of PIK3CA, which is located in 3q26.3, in cervical tumorigenesis. PIK3CA is known to be involved in the PI 3-kinase/AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. The results of comparative genomic hybridization show that the 3q26.3 amplification was the most consistent chromosomal aberration in primary tissues of cervical carcinoma, and a positive correlation between an increased copy number of PIK3CA (detected by competitive PCR) and 3q26.3 amplification was found in tumor tissues and in cervical cancer cell lines. In cervical cancer cell lines harboring amplified PIK3CA, the expression of gene product (p110α) of PIK3CA was increased, and was subsequently associated with high kinase activity. In addition, transformation phenotypes in these lines, including increased cell growth and decreased apoptosis, were found to be significantly affected by the treatment of specific PI 3-kinase inhibitor, suggesting that increased expression of PIK3CA in cervical cancer may result in promoting cell proliferation and reducing apoptosis. These evidences support that PIK3CA is an oncogene in cervical cancer and PIK3CA amplification may be linked to cervical tumorigenesis.

KW - 3q26.3

KW - AKT

KW - Cervical cancer

KW - p110α

KW - PI 3-kinase

KW - PIK3CA

UR - http://www.scopus.com/inward/record.url?scp=0034713390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034713390&partnerID=8YFLogxK

M3 - Article

C2 - 10851074

AN - SCOPUS:0034713390

VL - 19

SP - 2739

EP - 2744

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 23

ER -