Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells

Shu Ling Tzeng, Yu W. Cheng, Ching H. Li, Young S. Lin, Hey C. Hsu, Jaw J. Kang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co- immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.

Original languageEnglish
Pages (from-to)15405-15411
Number of pages7
JournalJournal of Biological Chemistry
Volume281
Issue number22
DOIs
Publication statusPublished - Jun 2 2006
Externally publishedYes

Fingerprint

Colonic Neoplasms
Colon
Cells
Yeast
Yeasts
Tissue
Proteins
HEK293 Cells
Cyclin D1
G1 Phase
Cell death
Immunoprecipitation
Cell Differentiation
Adenocarcinoma
Cell Death
Genes
Gene Expression
DNA
human DAP protein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells. / Tzeng, Shu Ling; Cheng, Yu W.; Li, Ching H.; Lin, Young S.; Hsu, Hey C.; Kang, Jaw J.

In: Journal of Biological Chemistry, Vol. 281, No. 22, 02.06.2006, p. 15405-15411.

Research output: Contribution to journalArticle

Tzeng, Shu Ling ; Cheng, Yu W. ; Li, Ching H. ; Lin, Young S. ; Hsu, Hey C. ; Kang, Jaw J. / Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 22. pp. 15405-15411.
@article{c8466c27189f4dfc9952ec7f9475ab8e,
title = "Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells",
abstract = "Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co- immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.",
author = "Tzeng, {Shu Ling} and Cheng, {Yu W.} and Li, {Ching H.} and Lin, {Young S.} and Hsu, {Hey C.} and Kang, {Jaw J.}",
year = "2006",
month = "6",
day = "2",
doi = "10.1074/jbc.M601807200",
language = "English",
volume = "281",
pages = "15405--15411",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "22",

}

TY - JOUR

T1 - Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells

AU - Tzeng, Shu Ling

AU - Cheng, Yu W.

AU - Li, Ching H.

AU - Lin, Young S.

AU - Hsu, Hey C.

AU - Kang, Jaw J.

PY - 2006/6/2

Y1 - 2006/6/2

N2 - Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co- immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.

AB - Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co- immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.

UR - http://www.scopus.com/inward/record.url?scp=33744951784&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744951784&partnerID=8YFLogxK

U2 - 10.1074/jbc.M601807200

DO - 10.1074/jbc.M601807200

M3 - Article

C2 - 16569639

AN - SCOPUS:33744951784

VL - 281

SP - 15405

EP - 15411

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 22

ER -