Physicochemical characterization of lens crystallins from the carp and biochemical comparison with other vertebrate and invertebrate crystallins

Shyh Horng Chiou, Wen Chang Chang, Fu Ming Pan, Tschining Chang, Tung Bin Lo

Research output: Contribution to journalArticle

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Abstract

Lens crystallins were isolated from the homogenate of carp (Cyprinus carpio) eye lenses by gel permeation chromatography and characterized by gel electrophoresis, immunodiffusion, amino acid analysis, circular dichroism, and protein sequence analysis. Three well-defined fractions corresponding to α/β-, β-, and γ-crystallins were obtained in relative weight percentages of 26, 22, and 52%. The native molecular masses of the purified fractions were determined to be 410, 60, and 20 kDa, respectively. The polypeptide compositions as determined by SDS gel electrophoresis revealed the substantial presence of β-crystallin polypeptides in the α-crystallin fraction; this is also evident in the fractionation of amphibian crystallins but is not common in the case of higher classes of vertebrates. The circular dichroism spectra indicate a predominant β-sheet structure in all three fractions, albeit with some contribution of α-helical structure in the γ-crystailin, the amino acid composition of which bears a resemblance to that of squid crystallin. Sequence comparison of carp γ-crystallin with frog and calf γ-crystallins indicates a high degree of homology in their N-terminal segments despite the dissimilarity of amino acid compositions and weak immunological cross-reactivity.

Original languageEnglish
Pages (from-to)751-759
Number of pages9
JournalJournal of Biochemistry
Volume101
Issue number3
DOIs
Publication statusPublished - Jan 1 1987
Externally publishedYes

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Crystallins
Carps
Invertebrates
Lenses
Vertebrates
Circular Dichroism
Electrophoresis
Amino Acids
Gels
Chemical analysis
Decapodiformes
Peptides
Crystalline Lens
Immunodiffusion
Protein Sequence Analysis
Gel permeation chromatography
Molecular mass
Amphibians
Fractionation
Anura

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Physicochemical characterization of lens crystallins from the carp and biochemical comparison with other vertebrate and invertebrate crystallins. / Chiou, Shyh Horng; Chang, Wen Chang; Pan, Fu Ming; Chang, Tschining; Lo, Tung Bin.

In: Journal of Biochemistry, Vol. 101, No. 3, 01.01.1987, p. 751-759.

Research output: Contribution to journalArticle

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