Physical and functional interactions between hnRNP K and PRMT family proteins

James Yi Hsin Chan, Tsai Yuan Hsieh, Shu Ting Liu, Wei Yuan Chou, Min Huey Chung, Shih Ming Huang

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalFEBS Letters
Volume583
Issue number2
DOIs
Publication statusPublished - Jan 22 2009

Keywords

  • HCV Core protein
  • hnRNP K
  • PRMT
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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