Physical and functional interactions between hnRNP K and PRMT family proteins

James Yi Hsin Chan, Tsai Yuan Hsieh, Shu Ting Liu, Wei Yuan Chou, Min Huey Chung, Shih Ming Huang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalFEBS Letters
Volume583
Issue number2
DOIs
Publication statusPublished - Jan 22 2009

Fingerprint

Heterogeneous-Nuclear Ribonucleoprotein K
Proteins
Methylation
Arginine
Nuclear Receptor Coactivators
Methyltransferases
Immunoprecipitation
Transcriptional Activation
Assays
Gene Expression

Keywords

  • HCV Core protein
  • hnRNP K
  • PRMT
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Chan, J. Y. H., Hsieh, T. Y., Liu, S. T., Chou, W. Y., Chung, M. H., & Huang, S. M. (2009). Physical and functional interactions between hnRNP K and PRMT family proteins. FEBS Letters, 583(2), 281-286. https://doi.org/10.1016/j.febslet.2008.12.025

Physical and functional interactions between hnRNP K and PRMT family proteins. / Chan, James Yi Hsin; Hsieh, Tsai Yuan; Liu, Shu Ting; Chou, Wei Yuan; Chung, Min Huey; Huang, Shih Ming.

In: FEBS Letters, Vol. 583, No. 2, 22.01.2009, p. 281-286.

Research output: Contribution to journalArticle

Chan, James Yi Hsin ; Hsieh, Tsai Yuan ; Liu, Shu Ting ; Chou, Wei Yuan ; Chung, Min Huey ; Huang, Shih Ming. / Physical and functional interactions between hnRNP K and PRMT family proteins. In: FEBS Letters. 2009 ; Vol. 583, No. 2. pp. 281-286.
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abstract = "The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).",
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AU - Chung, Min Huey

AU - Huang, Shih Ming

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N2 - The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).

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KW - HCV Core protein

KW - hnRNP K

KW - PRMT

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