Photosensitizer targeting: Mitochondrion-targeted photosensitizer enhances mitochondrial reactive oxygen species and mitochondrial calcium-mediated apoptosis

Tsung I. Peng, Cheng Jen Chang, Shuo Bin Jou, Chuen Mao Yang, Mei Jie Jou

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using laser scanning imaging microscopy, our previous studies have demonstrated visible laser irradiation induced significant generation of mitochondrial reactive oxygen species (mROS) and apoptosis. In addition, photodynamic therapy (PDT) with a mitochondrion-targeted photosensitizer, benzoporphyrin derivative monoacid ring A (BPD-MA), significantly enhanced visible laser irradiation induced mitochondrial dysfunction and apoptosis. To validate the critical role of the mitochondrion as well as how mitochondrial mechanisms were involved in the PDT of BPD-MA, subcellular PDT with BPD-MA were performed using region irradiation and recorded by multi-photon imaging microscopy coupled with mitochondrial specific fluorescent probes in single live C6 glioma cells. Specifically, mROS and mitochondrial free calcium (mCa2+) were simultaneously measured from cells received subcellular PDT with BPD-MA in different compartments including the mitochondrion, the cytosol and the nucleus. Results revealed that significant formation of mROS accompanied by elevation of mCa2+ was found only in the mitochondrial PDT with BPD-MA. Subsequently, mitochondrial PDT with BPD-MA resulted in severe mitochondrial swelling, plasma membrane blebing and apoptotic death. We conclude that via augmented mROS formation and mCa 2+ elevation PDT with a mitochondrion-targeted photosensitizer extensively enhances mitochondria-mediated apoptosis and, thus, may provide high eradication efficacy for clinical cancer treatment.

Original languageEnglish
Pages (from-to)1377-1384
Number of pages8
JournalOptical and Quantum Electronics
Volume37
Issue number13-15
DOIs
Publication statusPublished - Dec 1 2005
Externally publishedYes

Fingerprint

Photodynamic therapy
Mitochondria
Photosensitizing Agents
Photosensitizers
mitochondria
apoptosis
Cell death
calcium
Calcium
therapy
Reactive Oxygen Species
Apoptosis
Oxygen
Derivatives
oxygen
rings
Laser beam effects
irradiation
Microscopic examination
microscopy

Keywords

  • Apoptosis
  • BPD-MA
  • mCa
  • Mitochondria
  • Mitochondrial swelling
  • mROS
  • Photodynamic therapy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

Photosensitizer targeting : Mitochondrion-targeted photosensitizer enhances mitochondrial reactive oxygen species and mitochondrial calcium-mediated apoptosis. / Peng, Tsung I.; Chang, Cheng Jen; Jou, Shuo Bin; Yang, Chuen Mao; Jou, Mei Jie.

In: Optical and Quantum Electronics, Vol. 37, No. 13-15, 01.12.2005, p. 1377-1384.

Research output: Contribution to journalArticle

@article{1026d9be01d646e3b6d4829e358e680c,
title = "Photosensitizer targeting: Mitochondrion-targeted photosensitizer enhances mitochondrial reactive oxygen species and mitochondrial calcium-mediated apoptosis",
abstract = "Using laser scanning imaging microscopy, our previous studies have demonstrated visible laser irradiation induced significant generation of mitochondrial reactive oxygen species (mROS) and apoptosis. In addition, photodynamic therapy (PDT) with a mitochondrion-targeted photosensitizer, benzoporphyrin derivative monoacid ring A (BPD-MA), significantly enhanced visible laser irradiation induced mitochondrial dysfunction and apoptosis. To validate the critical role of the mitochondrion as well as how mitochondrial mechanisms were involved in the PDT of BPD-MA, subcellular PDT with BPD-MA were performed using region irradiation and recorded by multi-photon imaging microscopy coupled with mitochondrial specific fluorescent probes in single live C6 glioma cells. Specifically, mROS and mitochondrial free calcium (mCa2+) were simultaneously measured from cells received subcellular PDT with BPD-MA in different compartments including the mitochondrion, the cytosol and the nucleus. Results revealed that significant formation of mROS accompanied by elevation of mCa2+ was found only in the mitochondrial PDT with BPD-MA. Subsequently, mitochondrial PDT with BPD-MA resulted in severe mitochondrial swelling, plasma membrane blebing and apoptotic death. We conclude that via augmented mROS formation and mCa 2+ elevation PDT with a mitochondrion-targeted photosensitizer extensively enhances mitochondria-mediated apoptosis and, thus, may provide high eradication efficacy for clinical cancer treatment.",
keywords = "Apoptosis, BPD-MA, mCa, Mitochondria, Mitochondrial swelling, mROS, Photodynamic therapy",
author = "Peng, {Tsung I.} and Chang, {Cheng Jen} and Jou, {Shuo Bin} and Yang, {Chuen Mao} and Jou, {Mei Jie}",
year = "2005",
month = "12",
day = "1",
doi = "10.1007/s11082-005-4217-2",
language = "English",
volume = "37",
pages = "1377--1384",
journal = "Optical and Quantum Electronics",
issn = "0306-8919",
publisher = "Springer New York",
number = "13-15",

}

TY - JOUR

T1 - Photosensitizer targeting

T2 - Mitochondrion-targeted photosensitizer enhances mitochondrial reactive oxygen species and mitochondrial calcium-mediated apoptosis

AU - Peng, Tsung I.

AU - Chang, Cheng Jen

AU - Jou, Shuo Bin

AU - Yang, Chuen Mao

AU - Jou, Mei Jie

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Using laser scanning imaging microscopy, our previous studies have demonstrated visible laser irradiation induced significant generation of mitochondrial reactive oxygen species (mROS) and apoptosis. In addition, photodynamic therapy (PDT) with a mitochondrion-targeted photosensitizer, benzoporphyrin derivative monoacid ring A (BPD-MA), significantly enhanced visible laser irradiation induced mitochondrial dysfunction and apoptosis. To validate the critical role of the mitochondrion as well as how mitochondrial mechanisms were involved in the PDT of BPD-MA, subcellular PDT with BPD-MA were performed using region irradiation and recorded by multi-photon imaging microscopy coupled with mitochondrial specific fluorescent probes in single live C6 glioma cells. Specifically, mROS and mitochondrial free calcium (mCa2+) were simultaneously measured from cells received subcellular PDT with BPD-MA in different compartments including the mitochondrion, the cytosol and the nucleus. Results revealed that significant formation of mROS accompanied by elevation of mCa2+ was found only in the mitochondrial PDT with BPD-MA. Subsequently, mitochondrial PDT with BPD-MA resulted in severe mitochondrial swelling, plasma membrane blebing and apoptotic death. We conclude that via augmented mROS formation and mCa 2+ elevation PDT with a mitochondrion-targeted photosensitizer extensively enhances mitochondria-mediated apoptosis and, thus, may provide high eradication efficacy for clinical cancer treatment.

AB - Using laser scanning imaging microscopy, our previous studies have demonstrated visible laser irradiation induced significant generation of mitochondrial reactive oxygen species (mROS) and apoptosis. In addition, photodynamic therapy (PDT) with a mitochondrion-targeted photosensitizer, benzoporphyrin derivative monoacid ring A (BPD-MA), significantly enhanced visible laser irradiation induced mitochondrial dysfunction and apoptosis. To validate the critical role of the mitochondrion as well as how mitochondrial mechanisms were involved in the PDT of BPD-MA, subcellular PDT with BPD-MA were performed using region irradiation and recorded by multi-photon imaging microscopy coupled with mitochondrial specific fluorescent probes in single live C6 glioma cells. Specifically, mROS and mitochondrial free calcium (mCa2+) were simultaneously measured from cells received subcellular PDT with BPD-MA in different compartments including the mitochondrion, the cytosol and the nucleus. Results revealed that significant formation of mROS accompanied by elevation of mCa2+ was found only in the mitochondrial PDT with BPD-MA. Subsequently, mitochondrial PDT with BPD-MA resulted in severe mitochondrial swelling, plasma membrane blebing and apoptotic death. We conclude that via augmented mROS formation and mCa 2+ elevation PDT with a mitochondrion-targeted photosensitizer extensively enhances mitochondria-mediated apoptosis and, thus, may provide high eradication efficacy for clinical cancer treatment.

KW - Apoptosis

KW - BPD-MA

KW - mCa

KW - Mitochondria

KW - Mitochondrial swelling

KW - mROS

KW - Photodynamic therapy

UR - http://www.scopus.com/inward/record.url?scp=31144454069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31144454069&partnerID=8YFLogxK

U2 - 10.1007/s11082-005-4217-2

DO - 10.1007/s11082-005-4217-2

M3 - Article

AN - SCOPUS:31144454069

VL - 37

SP - 1377

EP - 1384

JO - Optical and Quantum Electronics

JF - Optical and Quantum Electronics

SN - 0306-8919

IS - 13-15

ER -