Photolysis of NSAIDs. II. Online LC-MS determination of photodegradants from carprofen

Fu A. Chen, Po Y. Wang, Kuo Ching Wen, Chau Yang Chen, An Bang Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Carprofen in a 10% aqueous ethanol solution at a concentration of 0.10 mg/mL was subjected to photo-irradiation under sunlight for 24 hr. Four photodegradants were separated and subsequently followed an online determination of their quasi molecular ions using an LC-MS method. The HPLC consisted an Inertsil 5 ODS-80A (2.1 mm i.d. × 150 mm) column, and the mobile phase was initially CH3CN: NH4OAc (20 mM in DI H2O) = 43: 57 (v/v). After 14 min, CH3CN: NH4OAc (20 mM in D.I. H2O) was changed to 54: 46 (v/v). The UV detector was set at 260 nm. The parameters of LC-MS for mass determination were optimized with an API electron spray interface with negative mode of polarity (ESI-). The chemical structures of the degradants were elucidated based on the m/z of the quasi molecular ions. Two degradants were found to proceed via an initial dechlorination with C-Cl cleavage. Dechlorination was observed to be competing with decarboxylation, i.e. either reaction is in accord with our previously reported result with a first-order photodecomposition kinetics of carprofen.

Original languageEnglish
Pages (from-to)186-190
Number of pages5
JournalJournal of Food and Drug Analysis
Volume11
Issue number3
Publication statusPublished - Sep 2003

Fingerprint

dechlorination
Photolysis
nonsteroidal anti-inflammatory agents
photolysis
Non-Steroidal Anti-Inflammatory Agents
Ions
ions
Decarboxylation
decarboxylation
Sunlight
chemical structure
detectors
solar radiation
Ethanol
irradiation
High Pressure Liquid Chromatography
ethanol
electrons
Electrons
kinetics

Keywords

  • Carprofen
  • Dechlorination
  • LC-MS
  • NSAIDs
  • Photolysis

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Chen, F. A., Wang, P. Y., Wen, K. C., Chen, C. Y., & Wu, A. B. (2003). Photolysis of NSAIDs. II. Online LC-MS determination of photodegradants from carprofen. Journal of Food and Drug Analysis, 11(3), 186-190.

Photolysis of NSAIDs. II. Online LC-MS determination of photodegradants from carprofen. / Chen, Fu A.; Wang, Po Y.; Wen, Kuo Ching; Chen, Chau Yang; Wu, An Bang.

In: Journal of Food and Drug Analysis, Vol. 11, No. 3, 09.2003, p. 186-190.

Research output: Contribution to journalArticle

Chen, FA, Wang, PY, Wen, KC, Chen, CY & Wu, AB 2003, 'Photolysis of NSAIDs. II. Online LC-MS determination of photodegradants from carprofen', Journal of Food and Drug Analysis, vol. 11, no. 3, pp. 186-190.
Chen, Fu A. ; Wang, Po Y. ; Wen, Kuo Ching ; Chen, Chau Yang ; Wu, An Bang. / Photolysis of NSAIDs. II. Online LC-MS determination of photodegradants from carprofen. In: Journal of Food and Drug Analysis. 2003 ; Vol. 11, No. 3. pp. 186-190.
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AB - Carprofen in a 10% aqueous ethanol solution at a concentration of 0.10 mg/mL was subjected to photo-irradiation under sunlight for 24 hr. Four photodegradants were separated and subsequently followed an online determination of their quasi molecular ions using an LC-MS method. The HPLC consisted an Inertsil 5 ODS-80A (2.1 mm i.d. × 150 mm) column, and the mobile phase was initially CH3CN: NH4OAc (20 mM in DI H2O) = 43: 57 (v/v). After 14 min, CH3CN: NH4OAc (20 mM in D.I. H2O) was changed to 54: 46 (v/v). The UV detector was set at 260 nm. The parameters of LC-MS for mass determination were optimized with an API electron spray interface with negative mode of polarity (ESI-). The chemical structures of the degradants were elucidated based on the m/z of the quasi molecular ions. Two degradants were found to proceed via an initial dechlorination with C-Cl cleavage. Dechlorination was observed to be competing with decarboxylation, i.e. either reaction is in accord with our previously reported result with a first-order photodecomposition kinetics of carprofen.

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