Photoinactivation of bladder tumor cells by methylene blue: Study of a variety of tumor and normal cells

D. S. Yu, S. Y. Chang, C. P. Ma

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Human bladder cancer cell lines, J82, Yen-87, Shen-87 and Zoa-88, and murine bladder cancer cell lines, MBT-2 and M1660, were used as target cells for dye-sensitized photoinactivation study in using methylene blue. Normal fibroblast cells, FB-1 and FB8490, were used as control group. The cytoplasmic activity of lactic dehydrogenase, soft agar clonogenic assay, and in vivo tumor growth, survival rate and tumor taking rate with or without photoinactivation were monitored and compared between different cell lines. Efficacy of photoinactivation was time-related and more than 90 per cent of cytotoxicity could be obtained within 60 minutes of illumination. The plateau of cytotoxicity curve could be achieved after staining for 30 minutes by methylene blue under the same illumination time. Normal fibroblasts had the same features with cancer cells. Photoinactivation of tumor cells showed significant inhibition of tumor growth and tumor taking rate in experimental animals. Survival rate was also significantly prolonged in the animals with tumor cells receiving photoinactivation. These results suggest that methylene blue-sensitized photoinactivation may be useful as an adjuvant photochemotherapy for superficial bladder cancer.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalJournal of Urology
Volume144
Issue number1
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

Fingerprint

Methylene Blue
Urinary Bladder Neoplasms
Neoplasms
Lighting
Cell Line
Fibroblasts
Photochemotherapy
Growth
Agar
Oxidoreductases
Milk
Coloring Agents
Staining and Labeling
Control Groups

ASJC Scopus subject areas

  • Urology

Cite this

Photoinactivation of bladder tumor cells by methylene blue : Study of a variety of tumor and normal cells. / Yu, D. S.; Chang, S. Y.; Ma, C. P.

In: Journal of Urology, Vol. 144, No. 1, 01.01.1990, p. 164-168.

Research output: Contribution to journalArticle

@article{02817759e3754635b13af66a9eca4681,
title = "Photoinactivation of bladder tumor cells by methylene blue: Study of a variety of tumor and normal cells",
abstract = "Human bladder cancer cell lines, J82, Yen-87, Shen-87 and Zoa-88, and murine bladder cancer cell lines, MBT-2 and M1660, were used as target cells for dye-sensitized photoinactivation study in using methylene blue. Normal fibroblast cells, FB-1 and FB8490, were used as control group. The cytoplasmic activity of lactic dehydrogenase, soft agar clonogenic assay, and in vivo tumor growth, survival rate and tumor taking rate with or without photoinactivation were monitored and compared between different cell lines. Efficacy of photoinactivation was time-related and more than 90 per cent of cytotoxicity could be obtained within 60 minutes of illumination. The plateau of cytotoxicity curve could be achieved after staining for 30 minutes by methylene blue under the same illumination time. Normal fibroblasts had the same features with cancer cells. Photoinactivation of tumor cells showed significant inhibition of tumor growth and tumor taking rate in experimental animals. Survival rate was also significantly prolonged in the animals with tumor cells receiving photoinactivation. These results suggest that methylene blue-sensitized photoinactivation may be useful as an adjuvant photochemotherapy for superficial bladder cancer.",
author = "Yu, {D. S.} and Chang, {S. Y.} and Ma, {C. P.}",
year = "1990",
month = "1",
day = "1",
doi = "10.1016/S0022-5347(17)39402-8",
language = "English",
volume = "144",
pages = "164--168",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Photoinactivation of bladder tumor cells by methylene blue

T2 - Study of a variety of tumor and normal cells

AU - Yu, D. S.

AU - Chang, S. Y.

AU - Ma, C. P.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Human bladder cancer cell lines, J82, Yen-87, Shen-87 and Zoa-88, and murine bladder cancer cell lines, MBT-2 and M1660, were used as target cells for dye-sensitized photoinactivation study in using methylene blue. Normal fibroblast cells, FB-1 and FB8490, were used as control group. The cytoplasmic activity of lactic dehydrogenase, soft agar clonogenic assay, and in vivo tumor growth, survival rate and tumor taking rate with or without photoinactivation were monitored and compared between different cell lines. Efficacy of photoinactivation was time-related and more than 90 per cent of cytotoxicity could be obtained within 60 minutes of illumination. The plateau of cytotoxicity curve could be achieved after staining for 30 minutes by methylene blue under the same illumination time. Normal fibroblasts had the same features with cancer cells. Photoinactivation of tumor cells showed significant inhibition of tumor growth and tumor taking rate in experimental animals. Survival rate was also significantly prolonged in the animals with tumor cells receiving photoinactivation. These results suggest that methylene blue-sensitized photoinactivation may be useful as an adjuvant photochemotherapy for superficial bladder cancer.

AB - Human bladder cancer cell lines, J82, Yen-87, Shen-87 and Zoa-88, and murine bladder cancer cell lines, MBT-2 and M1660, were used as target cells for dye-sensitized photoinactivation study in using methylene blue. Normal fibroblast cells, FB-1 and FB8490, were used as control group. The cytoplasmic activity of lactic dehydrogenase, soft agar clonogenic assay, and in vivo tumor growth, survival rate and tumor taking rate with or without photoinactivation were monitored and compared between different cell lines. Efficacy of photoinactivation was time-related and more than 90 per cent of cytotoxicity could be obtained within 60 minutes of illumination. The plateau of cytotoxicity curve could be achieved after staining for 30 minutes by methylene blue under the same illumination time. Normal fibroblasts had the same features with cancer cells. Photoinactivation of tumor cells showed significant inhibition of tumor growth and tumor taking rate in experimental animals. Survival rate was also significantly prolonged in the animals with tumor cells receiving photoinactivation. These results suggest that methylene blue-sensitized photoinactivation may be useful as an adjuvant photochemotherapy for superficial bladder cancer.

UR - http://www.scopus.com/inward/record.url?scp=0025369302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025369302&partnerID=8YFLogxK

U2 - 10.1016/S0022-5347(17)39402-8

DO - 10.1016/S0022-5347(17)39402-8

M3 - Article

C2 - 2359171

AN - SCOPUS:0025369302

VL - 144

SP - 164

EP - 168

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 1

ER -