Photodynamic inactivation of chlorin e6-loaded CTAB-liposomes against Candida albicans

Yu Tsai Yang, Hsiung-Fei Chien, Po Han Chang, Y. C. Chen, Michael Jay, Tsui-Min Tsai, Chin Tin Chen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background and Objectives Antimicrobial photodynamic inactivation (PDI) is a promising therapeutic modality for the treatment of local infections. To increase the efficacy of PDI, chlorine e6 (Ce6) was encapsulated in cationic CTAB-liposomes composed of various ratios of dimyristoyl-sn-glycero- phosphatidylcholine (DMPC) and the cationic surfactant, cetyltrimethyl ammonium bromide (CTAB). The PDI efficacy of the liposomal-Ce6 was assessed in vitro against susceptible and drug-resistant clinical isolates of Candida albicans (C. albicans) as well as in infected burn wounds. Study Design/Materials and Methods Ce6 was encapsulated in CTAB-liposomes by the film hydration method. Particle size distribution and zeta potential of the cationic liposomes were measured using a Zetasizer Nano-ZS. UV-visible spectra were used to measure lipid/Ce6 (L/C) ratio and drug entrapment efficiency while differential scanning calorimetry (DSC) was used to study the thermotropic behavior of DMPC liposomes upon CTAB addition. In vivo PDI efficacy was carried out in an infected burn wound using a rat model. Results The increase in zeta potential and a shift in the phase transition temperature (Tm) upon CTAB addition confirmed its entrapment within the lipid bilayers of the liposome. Meanwhile, the CTAB addition did not affect the Ce6 entrapment efficiency and physical attributes of the liposomes. In vitro studies showed that the PDI effect of the Ce6-loaded CTAB-liposomes was dependent on the lipid to Ce6 molar ratio (L/C), particle size and the concentration of CTAB in the liposomes. The lower L/C ratio and smaller liposomes exerted significantly higher PDI effects. In addition, an increase in the CTAB to lipid ratio led to a significant increase in the PDI effect of Ce6 against susceptible and drug-resistant clinical isolates of C. albicans after light illumination. Conclusions Our results indicate that a low L/C ratio, high positive charge, and small particle size of CTAB-liposomes significantly enhances their PDI efficacy against C. albicans.

Original languageEnglish
Pages (from-to)175-185
Number of pages11
JournalLasers in Surgery and Medicine
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Candida albicans
Liposomes
Lipids
Particle Size
Phosphatidylcholines
ammonium bromide
chlorin e6
Pharmaceutical Preparations
Transition Temperature
Phase Transition
Differential Scanning Calorimetry
Wounds and Injuries
Lipid Bilayers
Lighting
Surface-Active Agents
Light
Therapeutics
Infection

Keywords

  • Candida albicans
  • CTAB
  • liposome
  • photodynamic inactivation

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Photodynamic inactivation of chlorin e6-loaded CTAB-liposomes against Candida albicans. / Yang, Yu Tsai; Chien, Hsiung-Fei; Chang, Po Han; Chen, Y. C.; Jay, Michael; Tsai, Tsui-Min; Chen, Chin Tin.

In: Lasers in Surgery and Medicine, Vol. 45, No. 3, 03.2013, p. 175-185.

Research output: Contribution to journalArticle

Yang, Yu Tsai ; Chien, Hsiung-Fei ; Chang, Po Han ; Chen, Y. C. ; Jay, Michael ; Tsai, Tsui-Min ; Chen, Chin Tin. / Photodynamic inactivation of chlorin e6-loaded CTAB-liposomes against Candida albicans. In: Lasers in Surgery and Medicine. 2013 ; Vol. 45, No. 3. pp. 175-185.
@article{a8a0996637934b97b658864d253c1be1,
title = "Photodynamic inactivation of chlorin e6-loaded CTAB-liposomes against Candida albicans",
abstract = "Background and Objectives Antimicrobial photodynamic inactivation (PDI) is a promising therapeutic modality for the treatment of local infections. To increase the efficacy of PDI, chlorine e6 (Ce6) was encapsulated in cationic CTAB-liposomes composed of various ratios of dimyristoyl-sn-glycero- phosphatidylcholine (DMPC) and the cationic surfactant, cetyltrimethyl ammonium bromide (CTAB). The PDI efficacy of the liposomal-Ce6 was assessed in vitro against susceptible and drug-resistant clinical isolates of Candida albicans (C. albicans) as well as in infected burn wounds. Study Design/Materials and Methods Ce6 was encapsulated in CTAB-liposomes by the film hydration method. Particle size distribution and zeta potential of the cationic liposomes were measured using a Zetasizer Nano-ZS. UV-visible spectra were used to measure lipid/Ce6 (L/C) ratio and drug entrapment efficiency while differential scanning calorimetry (DSC) was used to study the thermotropic behavior of DMPC liposomes upon CTAB addition. In vivo PDI efficacy was carried out in an infected burn wound using a rat model. Results The increase in zeta potential and a shift in the phase transition temperature (Tm) upon CTAB addition confirmed its entrapment within the lipid bilayers of the liposome. Meanwhile, the CTAB addition did not affect the Ce6 entrapment efficiency and physical attributes of the liposomes. In vitro studies showed that the PDI effect of the Ce6-loaded CTAB-liposomes was dependent on the lipid to Ce6 molar ratio (L/C), particle size and the concentration of CTAB in the liposomes. The lower L/C ratio and smaller liposomes exerted significantly higher PDI effects. In addition, an increase in the CTAB to lipid ratio led to a significant increase in the PDI effect of Ce6 against susceptible and drug-resistant clinical isolates of C. albicans after light illumination. Conclusions Our results indicate that a low L/C ratio, high positive charge, and small particle size of CTAB-liposomes significantly enhances their PDI efficacy against C. albicans.",
keywords = "Candida albicans, CTAB, liposome, photodynamic inactivation",
author = "Yang, {Yu Tsai} and Hsiung-Fei Chien and Chang, {Po Han} and Chen, {Y. C.} and Michael Jay and Tsui-Min Tsai and Chen, {Chin Tin}",
year = "2013",
month = "3",
doi = "10.1002/lsm.22124",
language = "English",
volume = "45",
pages = "175--185",
journal = "Lasers in Surgery and Medicine",
issn = "0196-8092",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Photodynamic inactivation of chlorin e6-loaded CTAB-liposomes against Candida albicans

AU - Yang, Yu Tsai

AU - Chien, Hsiung-Fei

AU - Chang, Po Han

AU - Chen, Y. C.

AU - Jay, Michael

AU - Tsai, Tsui-Min

AU - Chen, Chin Tin

PY - 2013/3

Y1 - 2013/3

N2 - Background and Objectives Antimicrobial photodynamic inactivation (PDI) is a promising therapeutic modality for the treatment of local infections. To increase the efficacy of PDI, chlorine e6 (Ce6) was encapsulated in cationic CTAB-liposomes composed of various ratios of dimyristoyl-sn-glycero- phosphatidylcholine (DMPC) and the cationic surfactant, cetyltrimethyl ammonium bromide (CTAB). The PDI efficacy of the liposomal-Ce6 was assessed in vitro against susceptible and drug-resistant clinical isolates of Candida albicans (C. albicans) as well as in infected burn wounds. Study Design/Materials and Methods Ce6 was encapsulated in CTAB-liposomes by the film hydration method. Particle size distribution and zeta potential of the cationic liposomes were measured using a Zetasizer Nano-ZS. UV-visible spectra were used to measure lipid/Ce6 (L/C) ratio and drug entrapment efficiency while differential scanning calorimetry (DSC) was used to study the thermotropic behavior of DMPC liposomes upon CTAB addition. In vivo PDI efficacy was carried out in an infected burn wound using a rat model. Results The increase in zeta potential and a shift in the phase transition temperature (Tm) upon CTAB addition confirmed its entrapment within the lipid bilayers of the liposome. Meanwhile, the CTAB addition did not affect the Ce6 entrapment efficiency and physical attributes of the liposomes. In vitro studies showed that the PDI effect of the Ce6-loaded CTAB-liposomes was dependent on the lipid to Ce6 molar ratio (L/C), particle size and the concentration of CTAB in the liposomes. The lower L/C ratio and smaller liposomes exerted significantly higher PDI effects. In addition, an increase in the CTAB to lipid ratio led to a significant increase in the PDI effect of Ce6 against susceptible and drug-resistant clinical isolates of C. albicans after light illumination. Conclusions Our results indicate that a low L/C ratio, high positive charge, and small particle size of CTAB-liposomes significantly enhances their PDI efficacy against C. albicans.

AB - Background and Objectives Antimicrobial photodynamic inactivation (PDI) is a promising therapeutic modality for the treatment of local infections. To increase the efficacy of PDI, chlorine e6 (Ce6) was encapsulated in cationic CTAB-liposomes composed of various ratios of dimyristoyl-sn-glycero- phosphatidylcholine (DMPC) and the cationic surfactant, cetyltrimethyl ammonium bromide (CTAB). The PDI efficacy of the liposomal-Ce6 was assessed in vitro against susceptible and drug-resistant clinical isolates of Candida albicans (C. albicans) as well as in infected burn wounds. Study Design/Materials and Methods Ce6 was encapsulated in CTAB-liposomes by the film hydration method. Particle size distribution and zeta potential of the cationic liposomes were measured using a Zetasizer Nano-ZS. UV-visible spectra were used to measure lipid/Ce6 (L/C) ratio and drug entrapment efficiency while differential scanning calorimetry (DSC) was used to study the thermotropic behavior of DMPC liposomes upon CTAB addition. In vivo PDI efficacy was carried out in an infected burn wound using a rat model. Results The increase in zeta potential and a shift in the phase transition temperature (Tm) upon CTAB addition confirmed its entrapment within the lipid bilayers of the liposome. Meanwhile, the CTAB addition did not affect the Ce6 entrapment efficiency and physical attributes of the liposomes. In vitro studies showed that the PDI effect of the Ce6-loaded CTAB-liposomes was dependent on the lipid to Ce6 molar ratio (L/C), particle size and the concentration of CTAB in the liposomes. The lower L/C ratio and smaller liposomes exerted significantly higher PDI effects. In addition, an increase in the CTAB to lipid ratio led to a significant increase in the PDI effect of Ce6 against susceptible and drug-resistant clinical isolates of C. albicans after light illumination. Conclusions Our results indicate that a low L/C ratio, high positive charge, and small particle size of CTAB-liposomes significantly enhances their PDI efficacy against C. albicans.

KW - Candida albicans

KW - CTAB

KW - liposome

KW - photodynamic inactivation

UR - http://www.scopus.com/inward/record.url?scp=84875324166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875324166&partnerID=8YFLogxK

U2 - 10.1002/lsm.22124

DO - 10.1002/lsm.22124

M3 - Article

C2 - 23508377

AN - SCOPUS:84875324166

VL - 45

SP - 175

EP - 185

JO - Lasers in Surgery and Medicine

JF - Lasers in Surgery and Medicine

SN - 0196-8092

IS - 3

ER -