Photobacterium damselae subsp. piscicida responds to antimicrobial peptides through phage-shock-protein A (PspA)-related extracytoplasmic stress response system

W. C. Tsai, T. Y. Kuo, C. Y. Lin, J. C. Lin, W. J. Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aims: To investigate whether Photobacterium damselae subsp. piscicida (Phdp) can sense and directly respond to the presence of cationic antimicrobial peptides (AMPs). Methods and Results: We performed proteomic methodologies to investigate the responsive proteins of Phdp on exposure to AMP Q6. Proteins significantly altered were analysed by two-dimensional gel electrophoresis (2-DE) and LC-ESI-Q-TOF MS/MS, thus resulting in five outer membrane proteins (OMPs), seven inner membrane proteins (IMPs) and 17 cytoplasmic proteins (CPs) identified. Quantitative real-time PCR was also applied to monitor the mRNA expression level of these target proteins. Conclusions: COG analysis revealed that upon exposure to AMP Q6, the majority of the upregulated proteins were involved in signal transduction mechanism, carbohydrate transport and metabolism, post-translational modification, protein turnover and chaperones, while the downregulated proteins were mainly related to energy production and conversion. Among them, phage-shock-protein A (PspA)-related stress response system was considered to play a crucial role. Significance and Impact of the Study: To the best of our knowledge, this is the first report elucidating Phdp AMP-response mechanism using proteomics approach. AMP-responsive proteins identified in this study could serve as attractive targets for developing more effective antimicrobial agents against Phdp and other marine bacterial pathogens.

Original languageEnglish
Pages (from-to)27-38
Number of pages12
JournalJournal of Applied Microbiology
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Antimicrobial peptides
  • Phage-shock-protein A (PspA)
  • Photobacterium damselae subsp. piscicida
  • Proteomics
  • Stress response

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

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