Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity

Yee Fun Su, Tsunghan Yang, Hoting Huang, Leroy-Fong Liu, Jaulang Hwang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Increasing evidence has pointed to an important role of SUMOylation in cell cycle regulation, especially for M phase. In the current studies, we have obtained evidence through in vitro studies that the master M phase regulator CDK1/cyclin B kinase phosphorylates the SUMOylation machinery component Ubc9, leading to its enhanced SUMOylation activity. First, we show that CDK1/cyclin B, but not many other cell cycle kinases such as CDK2/cyclin E, ERK1, ERK2, PKA and JNK2/SAPK1, specifically enhances SUMOylation activity. Second, CDK1/cyclin B phosphorylates the SUMOylation machinery component Ubc9, but not SAE1/SAE2 or SUMO1. Third, CDK1/cyclin B-phosphorylated Ubc9 exhibits increased SUMOylation activity and elevated accumulation of the Ubc9-SUMO1 thioester conjugate. Fourth, CDK1/cyclin B enhances SUMOylation activity through phosphorylation of Ubc9 at serine 71. These studies demonstrate for the first time that the cell cycle-specific kinase CDK1/cyclin B phosphorylates a SUMOylation machinery component to increase its overall SUMOylation activity, suggesting that SUMOylation is part of the cell cycle program orchestrated by CDK1 through Ubc9.

Original languageEnglish
Article numbere34250
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 3 2012
Externally publishedYes

Fingerprint

Sumoylation
Cyclin B
Phosphorylation
cyclins
phosphorylation
Cells
cell cycle
Machinery
Phosphotransferases
phosphotransferases (kinases)
Cell Cycle
mitosis
Cyclin E
Cell Division
Serine
in vitro studies
serine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity. / Su, Yee Fun; Yang, Tsunghan; Huang, Hoting; Liu, Leroy-Fong; Hwang, Jaulang.

In: PLoS One, Vol. 7, No. 4, e34250, 03.04.2012.

Research output: Contribution to journalArticle

Su, Yee Fun ; Yang, Tsunghan ; Huang, Hoting ; Liu, Leroy-Fong ; Hwang, Jaulang. / Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity. In: PLoS One. 2012 ; Vol. 7, No. 4.
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