Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos

Kai Yun Huang, Gen Der Chen, Chia Hsiung Cheng, Kuan Ya Liao, Chin Chun Hung, Geen Dong Chang, Pung Pung Hwang, Shu Yu Lin, Ming Chieh Tsai, Kay Hooi Khoo, Ming Ting Lee, Chang Jen Huang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Mammalian M6A, a member of the proteolipid protein (PLP/DM20) family expressed in neurons, was first isolated by expression cloning with a monoclonal antibody. Overexpression of M6A was shown to induce filopodium formation in neuronal cells; however, the underlying mechanism of is largely unknown. Possibly due to gene duplication, there are two M6A paralogs, M6Aa and M6Ab, in the zebrafish genome. In the present study, we used the zebrafish as a model system to investigate the role of zebrafish M6Ab in filopodium formation in PC12 cells and neurite outgrowth in zebrafish embryos. Methodology/Principal Findings: We demonstrated that zebrafish M6Ab promoted extensive filopodium formation in NGF-treated PC12 cells, which is similar to the function of mammalian M6A. Phosphorylation at serine 263 of zebrafish M6Ab contributed to this induction. Transfection of the S263A mutant protein greatly reduced filopodium formation in PC12 cells. In zebrafish embryos, only S263D could induce neurite outgrowth. Conclusions/Significance: Our results reveal that the phosphorylation status of zebrafish M6Ab at serine 263 is critical for its role in regulating filopodium formation and neurite outgrowth.

Original languageEnglish
Article numbere26461
JournalPLoS One
Volume6
Issue number10
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

pseudopodia
Phosphorylation
Pseudopodia
neurites
PC12 Cells
Zebrafish
Danio rerio
serine
Serine
phosphorylation
embryo (animal)
Embryonic Structures
Genes
Proteolipids
Cloning
Nerve Growth Factor
Mutant Proteins
Neurons
Monoclonal Antibodies
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos. / Huang, Kai Yun; Chen, Gen Der; Cheng, Chia Hsiung; Liao, Kuan Ya; Hung, Chin Chun; Chang, Geen Dong; Hwang, Pung Pung; Lin, Shu Yu; Tsai, Ming Chieh; Khoo, Kay Hooi; Lee, Ming Ting; Huang, Chang Jen.

In: PLoS One, Vol. 6, No. 10, e26461, 2011.

Research output: Contribution to journalArticle

Huang, KY, Chen, GD, Cheng, CH, Liao, KY, Hung, CC, Chang, GD, Hwang, PP, Lin, SY, Tsai, MC, Khoo, KH, Lee, MT & Huang, CJ 2011, 'Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos', PLoS One, vol. 6, no. 10, e26461. https://doi.org/10.1371/journal.pone.0026461
Huang, Kai Yun ; Chen, Gen Der ; Cheng, Chia Hsiung ; Liao, Kuan Ya ; Hung, Chin Chun ; Chang, Geen Dong ; Hwang, Pung Pung ; Lin, Shu Yu ; Tsai, Ming Chieh ; Khoo, Kay Hooi ; Lee, Ming Ting ; Huang, Chang Jen. / Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos. In: PLoS One. 2011 ; Vol. 6, No. 10.
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AU - Huang, Kai Yun

AU - Chen, Gen Der

AU - Cheng, Chia Hsiung

AU - Liao, Kuan Ya

AU - Hung, Chin Chun

AU - Chang, Geen Dong

AU - Hwang, Pung Pung

AU - Lin, Shu Yu

AU - Tsai, Ming Chieh

AU - Khoo, Kay Hooi

AU - Lee, Ming Ting

AU - Huang, Chang Jen

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