Phosphorylation-dependent SUMOylation of the transcription factor NF-E2

Yee Fun Su; Yu Chiau Shyu; Che Kun James Shen; Jaulang Hwang

doi:10.1371/journal.pone.0044608

Phosphorylation-dependent SUMOylation of the transcription factor NF-E2

Yee Fun Su, Yu Chiau Shyu, Che Kun James Shen, Jaulang Hwang

Department of Biochemistry and Molecular Cell Biology

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.

Original language	English
Article number	e44608
Journal	PLoS One
Volume	7
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0044608
Publication status	Published - Sep 10 2012

Fingerprint

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

Cite this

Su, Y. F., Shyu, Y. C., Shen, C. K. J., & Hwang, J. (2012). Phosphorylation-dependent SUMOylation of the transcription factor NF-E2. PLoS One, 7(9), [e44608]. https://doi.org/10.1371/journal.pone.0044608

@article{62380e960a874061ac5ba52303f8aec3,

title = "Phosphorylation-dependent SUMOylation of the transcription factor NF-E2",

abstract = "Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.",

author = "Su, {Yee Fun} and Shyu, {Yu Chiau} and Shen, {Che Kun James} and Jaulang Hwang",

year = "2012",

month = sep,

day = "10",

doi = "10.1371/journal.pone.0044608",

language = "English",

volume = "7",

journal = "PLoS One",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "9",

}

TY - JOUR

T1 - Phosphorylation-dependent SUMOylation of the transcription factor NF-E2

AU - Su, Yee Fun

AU - Shyu, Yu Chiau

AU - Shen, Che Kun James

AU - Hwang, Jaulang

PY - 2012/9/10

Y1 - 2012/9/10

N2 - Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.

AB - Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.

UR - http://www.scopus.com/inward/record.url?scp=84874725492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874725492&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0044608

DO - 10.1371/journal.pone.0044608

M3 - Article

C2 - 22970264

AN - SCOPUS:84874725492

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e44608

ER -

Access to Document

10.1371/journal.pone.0044608