Abstract
Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.
| Original language | English |
|---|---|
| Article number | e44608 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 10 2012 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Phosphorylation-dependent SUMOylation of the transcription factor NF-E2. / Su, Yee Fun; Shyu, Yu Chiau; Shen, Che Kun James; Hwang, Jaulang.
In: PLoS One, Vol. 7, No. 9, e44608, 10.09.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphorylation-dependent SUMOylation of the transcription factor NF-E2
AU - Su, Yee Fun
AU - Shyu, Yu Chiau
AU - Shen, Che Kun James
AU - Hwang, Jaulang
PY - 2012/9/10
Y1 - 2012/9/10
N2 - Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.
AB - Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.
UR - http://www.scopus.com/inward/record.url?scp=84874725492&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874725492&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0044608
DO - 10.1371/journal.pone.0044608
M3 - Article
C2 - 22970264
AN - SCOPUS:84874725492
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 9
M1 - e44608
ER -