Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species

Kevin Coward, C. P. Ponting, H. Y. Chang, O. Hibbitt, P. Savolainen, K. T. Jones, J. Parrington

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The activation of the egg to begin development into an embryo is triggered by a sperm-induced increase in intracellular egg Ca2+. There has been much controversy about how the sperm induces this fundamental developmental event, but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCζ. Since the discovery of PLCζ, it has been unclear whether its role in triggering egg activation is common to all vertebrates, or is confined to mammals. Here, we demonstrate for the first time that PLCζ is present in a non-mammalian vertebrate. Using genomic and cDNA databases, we have identified the cDNA encoding a PLCζ orthologue in the domestic chicken that, like the mammalian isoforms, is a testis-specific gene. The chicken PLCζ cDNA is 2152bp in size and encodes an open reading frame of 639 amino acids. When injected into mouse oocytes, chicken PLCζ cRNA triggers Ca2+ oscillations, indicating that it has functional properties similar to those of mammalian PLCζ. Our findings suggest that PLCζ may have a universal role in triggering egg activation in vertebrates.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalReproduction
Volume130
Issue number2
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Type C Phospholipases
Ovum
Mammals
Vertebrates
Chickens
Complementary DNA
Spermatozoa
Testis
Complementary RNA
Open Reading Frames
Oocytes
Embryonic Development
Protein Isoforms
Databases
Amino Acids
Genes

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Cell Biology
  • Endocrinology
  • Embryology

Cite this

Coward, K., Ponting, C. P., Chang, H. Y., Hibbitt, O., Savolainen, P., Jones, K. T., & Parrington, J. (2005). Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. Reproduction, 130(2), 157-163. https://doi.org/10.1530/rep.1.00707

Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. / Coward, Kevin; Ponting, C. P.; Chang, H. Y.; Hibbitt, O.; Savolainen, P.; Jones, K. T.; Parrington, J.

In: Reproduction, Vol. 130, No. 2, 08.2005, p. 157-163.

Research output: Contribution to journalArticle

Coward, K, Ponting, CP, Chang, HY, Hibbitt, O, Savolainen, P, Jones, KT & Parrington, J 2005, 'Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species', Reproduction, vol. 130, no. 2, pp. 157-163. https://doi.org/10.1530/rep.1.00707
Coward K, Ponting CP, Chang HY, Hibbitt O, Savolainen P, Jones KT et al. Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. Reproduction. 2005 Aug;130(2):157-163. https://doi.org/10.1530/rep.1.00707
Coward, Kevin ; Ponting, C. P. ; Chang, H. Y. ; Hibbitt, O. ; Savolainen, P. ; Jones, K. T. ; Parrington, J. / Phospholipase Cζ, the trigger egg activation in mammals, is present in a non-mammalian species. In: Reproduction. 2005 ; Vol. 130, No. 2. pp. 157-163.
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