Phosphoinositide 3-Kinase Is Involved in Mediating the Anti-inflammation Effects of Vasopressin

Woan Ching Jan, Ming Chang Kao, Chen Hsien Yang, Ya Ying Chang, Chun Jen Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Vasopressin possesses potent anti-inflammatory capacity. Phosphoinositide 3-kinase (PI3K) and its downstream activator Akt contribute to endogenous anti-inflammation capacity. We sought to elucidate whether PI3K is involved in mediating the anti-inflammation effects of vasopressin. Macrophages (RAW264.7 cells) were randomized to receive endotoxin, endotoxin plus vasopressin, or endotoxin plus vasopressin plus the nonselective PI3K inhibitor (LY294002) or the selective isoform inhibitor of PI3Kα (PIK-75), PI3Kβ (TGX-221), PI3Kδ (IC-87114), or PI3Kγ (AS-252424). Compared to macrophages treated with endotoxin, the concentrations of cytokines (tumor necrosis factor-α, interleukin-6) and chemokine (macrophage inflammatory protein-2) in macrophages treated with endotoxin plus vasopressin were significantly lower (all P < 0.05). The concentrations of phosphorylated nuclear factor-κB p65 (p-NF-κB p65) in nuclear extracts and phosphorylated inhibitor-κBα (p-I-κBα) in cytosolic extracts as well as NF-κB-DNA binding activity were also lower (all P < 0.05). Of note, except for macrophages treated with endotoxin plus vasopressin plus PIK-75, the concentrations of cytokines, chemokine, p-NF-κB p65, and p-I-κBα as well as NF-κB-DNA binding activity in macrophages treated with endotoxin plus vasopressin plus LY294002, TGX-221, IC-87114, or AS-252424 were significantly higher than those in macrophages treated with endotoxin plus vasopressin (all P < 0.05). In contrast, the phosphorylated Akt concentration in macrophages treated with endotoxin plus vasopressin was significantly higher than that in macrophages treated with endotoxin or in macrophages treated with endotoxin plus vasopressin plus LY294002, TGX-221, IC-87114, or AS-252424, but not PIK-75. These data confirmed that PI3K, especially the isoforms of PI3Kβ, PI3Kδ, and PI3Kγ, is involved in mediating the anti-inflammatory effects of vasopressin.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalInflammation
Volume40
Issue number2
DOIs
Publication statusPublished - Apr 1 2017
Externally publishedYes

Fingerprint

1-Phosphatidylinositol 4-Kinase
Vasopressins
Endotoxins
Inflammation
Macrophages
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
B-Form DNA
Chemokines
Protein Isoforms
Anti-Inflammatory Agents
Chemokine CXCL2
Cytokines
Interleukin-6
Tumor Necrosis Factor-alpha

Keywords

  • chemokine
  • cytokine
  • endotoxin
  • macrophages
  • NF-κB
  • vasopressin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Phosphoinositide 3-Kinase Is Involved in Mediating the Anti-inflammation Effects of Vasopressin. / Jan, Woan Ching; Kao, Ming Chang; Yang, Chen Hsien; Chang, Ya Ying; Huang, Chun Jen.

In: Inflammation, Vol. 40, No. 2, 01.04.2017, p. 435-441.

Research output: Contribution to journalArticle

Jan, Woan Ching ; Kao, Ming Chang ; Yang, Chen Hsien ; Chang, Ya Ying ; Huang, Chun Jen. / Phosphoinositide 3-Kinase Is Involved in Mediating the Anti-inflammation Effects of Vasopressin. In: Inflammation. 2017 ; Vol. 40, No. 2. pp. 435-441.
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