Phosphine-induced oxidative damage in rats: Role of glutathione

Ching Hung Hsu, Bei Ching Chi, Ming Yie Liu, Jih Heng Li, Chiou Jong Chen, Ruey Yu Chen

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Phosphine (PH3), generated from aluminium, magnesium and zinc phosphide, is a widely used pesticide. PH3 induces oxidative stress in insects, mammalian cells, animals, and humans. The involvement of glutathione (GSH) in PH3-induced oxidative toxicity is controversial. GSH levels in various tested tissues were reduced in aluminium phosphide-poisoned rats and humans, while the levels remained unchanged in insects and mammalian cells. This study examines the effectiveness of endogenous GSH as a protective agent against PH3-induced oxidative damage in rats. The association of PH3-induced nephrotoxicity and cardiotoxicity with free radical production was also tested. Male Wistar rats, administered intraperitoneally (I.P.) with PH3 at 4 mg/kg, were evaluated 30 min after treatment for PH3 toxicity to organs. PH3 significantly decreased GSH, GSH peroxidase and catalase, while significantly increased lipid peroxidation (as malondialdehyde and 4-hydroxyalkenals), DNA oxidation (as 8-hydroxydeoxyguaonsoine) and superoxide dismutase (SOD) levels in kidney and heart. These changes were significantly alleviated by melatonin (10 mg/kg I.P., 30 min before PH3), with the exception of SOD activity in heart tissue. The study also found that buthionine sulfoximine (1 g/kg I.P., 24 h before PH3) significantly enhanced the effect of PH3 on GSH loss and lipid peroxidation elevation in lung. These findings indicate that (1) endogenous GSH plays a crucial role as a protective factor in modulating PH3-induced oxidative damage, and (2) PH3 could injure kidney and heart (as noted earlier with brain, liver and lung) via oxidative stress and the antioxidant melatonin effectively prevents the damage.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalToxicology
Volume179
Issue number1-2
DOIs
Publication statusPublished - Sep 30 2002

Fingerprint

phosphine
Glutathione
Rats
Oxidative stress
Melatonin
Lipid Peroxidation
Superoxide Dismutase
Toxicity
Insects
Oxidative Stress
Cells
Tissue
Buthionine Sulfoximine
Kidney
Lipids
Protective Agents
Lung
Malondialdehyde
Aluminum
Pesticides

Keywords

  • Glutathione
  • Oxidative damage
  • Phosphine
  • Rats

ASJC Scopus subject areas

  • Toxicology

Cite this

Phosphine-induced oxidative damage in rats : Role of glutathione. / Hsu, Ching Hung; Chi, Bei Ching; Liu, Ming Yie; Li, Jih Heng; Chen, Chiou Jong; Chen, Ruey Yu.

In: Toxicology, Vol. 179, No. 1-2, 30.09.2002, p. 1-8.

Research output: Contribution to journalArticle

Hsu, Ching Hung ; Chi, Bei Ching ; Liu, Ming Yie ; Li, Jih Heng ; Chen, Chiou Jong ; Chen, Ruey Yu. / Phosphine-induced oxidative damage in rats : Role of glutathione. In: Toxicology. 2002 ; Vol. 179, No. 1-2. pp. 1-8.
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