Pharmacokinetics of di-isononyl phthalate in freely moving rats by UPLC-MS/MS

Mei Ling Hou, Li Wen Chang, Chang Jung Chiang, Yang Hwei Tsuang, Chi Hung Lin, Tung Hu Tsai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Di-isononyl phthalate (DINP) is a general-purpose plasticizer for polyvinyl chloride. However, this industrial chemical plasticizer used as a clouding agent has recently contaminated food and beverages that had been inspected by Taiwan Food and Drug Administration. This study develops a sensitive and specific method combining ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-MS/MS) to investigate the pharmacokinetics of DINP in freely moving rats. Multiple reaction monitoring (MRM) was used to monitor the transition of the protonated molecule m/z of 419 [M+H]+ to the product ion 149 for DINP. The analyte was analyzed by UPLC-MS/MS with C18 column (100 × 2.1 mm, 1.7 μm) which was equilibrated and eluted with an isocratic mixture of acetonitrile-ammonium acetate water solution (90:10, v/v) at a flow rate of 0.3 mL/min. Linear calibration curves were obtained for DINP concentration ranges of 0.05-2.5 μg/mL in plasma and feces. The feces were homogenized mechanically using 50% acetonitrile as the medium. The pharmacokinetic curve demonstrates that the disposition of DINP in rat plasma was fitted well by the two-compartment model after DINP administration (10 mg/kg, i.v.). The elimination half-life of DINP was 364 ± 146 min and 150 ± 58 min for intravenous (10 mg/kg) and oral (100 mg/kg) administration, respectively. The pharmacokinetic data indicate that the oral bioavailability of DINP in freely moving rats was about 1.19%. The total DINP excretion up to 48 h was 13.64 ± 3.99% in feces.

Original languageEnglish
Pages (from-to)36-43
Number of pages8
JournalInternational Journal of Pharmaceutics
Volume450
Issue number1-2
DOIs
Publication statusPublished - Jun 25 2013

Fingerprint

Pharmacokinetics
Feces
Plasticizers
phthalic acid
Food and Beverages
Electrospray Ionization Mass Spectrometry
United States Food and Drug Administration
Tandem Mass Spectrometry
Taiwan
Polyvinyl Chloride
Liquid Chromatography
Calibration
Biological Availability
Half-Life
Ions
Water

Keywords

  • Di-isononyl phthalate
  • Food addition
  • Pharmacokinetics
  • Plasticizer
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Pharmacokinetics of di-isononyl phthalate in freely moving rats by UPLC-MS/MS. / Hou, Mei Ling; Chang, Li Wen; Chiang, Chang Jung; Tsuang, Yang Hwei; Lin, Chi Hung; Tsai, Tung Hu.

In: International Journal of Pharmaceutics, Vol. 450, No. 1-2, 25.06.2013, p. 36-43.

Research output: Contribution to journalArticle

Hou, Mei Ling ; Chang, Li Wen ; Chiang, Chang Jung ; Tsuang, Yang Hwei ; Lin, Chi Hung ; Tsai, Tung Hu. / Pharmacokinetics of di-isononyl phthalate in freely moving rats by UPLC-MS/MS. In: International Journal of Pharmaceutics. 2013 ; Vol. 450, No. 1-2. pp. 36-43.
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