Pharmacokinetic study of florfenicol in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography equipped with UV detector

Yu Wen Hung, Yu Hsing Lin, Ming Hui Chen, Way Shyan Wang, Ching Feng Chiu, Chien Chao Chiu, Hsuan Wen Chiu, Wei Huang Tsai, Shao Wen Hung

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of the study was to develop a simple, rapid, and reliable technique to detect the pharmacokinetics and the residual of florfenicol (FFC) in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography (HPLC) with UV detector. The results showed that the concentration-time course of the FFC was described by one-compartment model with 1st order absorption in the blood. The retention time of FFC was 7.3 min. The limit of detection of FFC concentration using our developed method was 0.01 mg kg−1 BW. After given single dose 10 mg kg−1 BW per day of FFC via oral administration for sturgeon, the main pharmacokinetic parameters in blood were found as following: Cmax (maximum concentration) was 10.9568 μg mL−1, Tmax (time to Cmax) was 10 h, AUC (area under the curve) was 251.064 μg × hr mL−1, t1/2 (half-life) was 12.1301 h, CL (total body clearance) was 0.0387 L kg−1, V/F (apparent volume of distribution) was 0.6777 L kg−1, and MRT (the mean residence time) was 23.7431 h. Additionally, the concentration-time course of the FFC was also described by two-compartment model with 1st order absorption in the muscle and liver. The recoveries of muscle and liver from sturgeon were 92–108% and 90–104%, respectively. Taken together, the results showed that the developed method technique may be a valuable tool was efficient in detecting the pharmacokinetics of FFC in Bester sturgeon.

Original languageEnglish
Pages (from-to)558-567
Number of pages10
JournalAquaculture
Volume495
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Huso
florfenicol
sturgeon
pharmacokinetics
detectors
liquid chromatography
high performance liquid chromatography
muscle
blood
half life
residence time
Huso huso
muscles
liver
detector
methodology
oral administration
detection limit

Keywords

  • Florfenicol
  • High performance liquid chromatography
  • Pharmacokinetics
  • Sturgeon

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Pharmacokinetic study of florfenicol in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography equipped with UV detector. / Hung, Yu Wen; Lin, Yu Hsing; Chen, Ming Hui; Wang, Way Shyan; Chiu, Ching Feng; Chiu, Chien Chao; Chiu, Hsuan Wen; Tsai, Wei Huang; Hung, Shao Wen.

In: Aquaculture, Vol. 495, 01.10.2018, p. 558-567.

Research output: Contribution to journalArticle

Hung, Yu Wen ; Lin, Yu Hsing ; Chen, Ming Hui ; Wang, Way Shyan ; Chiu, Ching Feng ; Chiu, Chien Chao ; Chiu, Hsuan Wen ; Tsai, Wei Huang ; Hung, Shao Wen. / Pharmacokinetic study of florfenicol in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography equipped with UV detector. In: Aquaculture. 2018 ; Vol. 495. pp. 558-567.
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abstract = "The aim of the study was to develop a simple, rapid, and reliable technique to detect the pharmacokinetics and the residual of florfenicol (FFC) in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography (HPLC) with UV detector. The results showed that the concentration-time course of the FFC was described by one-compartment model with 1st order absorption in the blood. The retention time of FFC was 7.3 min. The limit of detection of FFC concentration using our developed method was 0.01 mg kg−1 BW. After given single dose 10 mg kg−1 BW per day of FFC via oral administration for sturgeon, the main pharmacokinetic parameters in blood were found as following: Cmax (maximum concentration) was 10.9568 μg mL−1, Tmax (time to Cmax) was 10 h, AUC (area under the curve) was 251.064 μg × hr mL−1, t1/2 (half-life) was 12.1301 h, CL (total body clearance) was 0.0387 L kg−1, V/F (apparent volume of distribution) was 0.6777 L kg−1, and MRT (the mean residence time) was 23.7431 h. Additionally, the concentration-time course of the FFC was also described by two-compartment model with 1st order absorption in the muscle and liver. The recoveries of muscle and liver from sturgeon were 92–108{\%} and 90–104{\%}, respectively. Taken together, the results showed that the developed method technique may be a valuable tool was efficient in detecting the pharmacokinetics of FFC in Bester sturgeon.",
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AU - Hung, Yu Wen

AU - Lin, Yu Hsing

AU - Chen, Ming Hui

AU - Wang, Way Shyan

AU - Chiu, Ching Feng

AU - Chiu, Chien Chao

AU - Chiu, Hsuan Wen

AU - Tsai, Wei Huang

AU - Hung, Shao Wen

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AB - The aim of the study was to develop a simple, rapid, and reliable technique to detect the pharmacokinetics and the residual of florfenicol (FFC) in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus by high performance liquid chromatography (HPLC) with UV detector. The results showed that the concentration-time course of the FFC was described by one-compartment model with 1st order absorption in the blood. The retention time of FFC was 7.3 min. The limit of detection of FFC concentration using our developed method was 0.01 mg kg−1 BW. After given single dose 10 mg kg−1 BW per day of FFC via oral administration for sturgeon, the main pharmacokinetic parameters in blood were found as following: Cmax (maximum concentration) was 10.9568 μg mL−1, Tmax (time to Cmax) was 10 h, AUC (area under the curve) was 251.064 μg × hr mL−1, t1/2 (half-life) was 12.1301 h, CL (total body clearance) was 0.0387 L kg−1, V/F (apparent volume of distribution) was 0.6777 L kg−1, and MRT (the mean residence time) was 23.7431 h. Additionally, the concentration-time course of the FFC was also described by two-compartment model with 1st order absorption in the muscle and liver. The recoveries of muscle and liver from sturgeon were 92–108% and 90–104%, respectively. Taken together, the results showed that the developed method technique may be a valuable tool was efficient in detecting the pharmacokinetics of FFC in Bester sturgeon.

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