Peritoneal natural killer cytotoxicity and CD25+CD3+ lymphocyte subpopulation are decreased in women with stage III-IV endometriosis

Hong Nerng Ho, Kuang Han Chao, Hsin Fu Chen, Ming Yih Wu, Yu Shih Yang, Tzu Yao Lee

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Abstract

We examined the lymphocytes of peripheral blood (PB) and peritoneal fluid (PF) of women with or without endometriosis to investigate the alteration of cytotoxic activity of natural killer (NK) cells and activation of T cells in the peritoneal cavity of women with endometriosis. A total 16 control women and 14 patients with stage III-IV endometriosis were selected on the basis of laparoscopic examination in National Taiwan University Hospital. The lymphocyte subpopulations (B cell, NK cell and T cell), including T-cell activation markers (CD69, CD25, HLADR), in PB and PF were analysed by dual-colour flow cytometry. The NK cytotoxicity of PB and PF mononuclear cells was evaluated by 51Cr release assay. There was significant decrease of NK cytotoxicity and CD25+CD3+lymphocyte subpopulation in PF of women with endometriosis compared with those without endometriosis. However, there was no difference in the proportion of NK cells in both PB and PF between women with and without endometriosis. Therefore, the decreased NK cytotoxicity PF of women with endometriosis was due to the functional defect, but not quantitative defect, of NK cells. The concomitant reduction of activated T cells in women with endometriosis might suggest its possible role in the defect of NK cytotoxicity.

Original languageEnglish
Pages (from-to)2671-2675
Number of pages5
JournalHuman Reproduction
Volume10
Issue number10
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Lymphocyte Subsets
HLA-DR Antigens
Endometriosis
Ascitic Fluid
Natural Killer Cells
T-Lymphocytes
Peritoneal Cavity
Taiwan
Flow Cytometry
B-Lymphocytes
Color
Lymphocytes

Keywords

  • CD25
  • CD69
  • Endometriosis
  • HLA-DR
  • Natural killer cytotoxicity

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Peritoneal natural killer cytotoxicity and CD25+CD3+ lymphocyte subpopulation are decreased in women with stage III-IV endometriosis. / Ho, Hong Nerng; Chao, Kuang Han; Chen, Hsin Fu; Wu, Ming Yih; Yang, Yu Shih; Lee, Tzu Yao.

In: Human Reproduction, Vol. 10, No. 10, 01.01.1995, p. 2671-2675.

Research output: Contribution to journalArticle

Ho, Hong Nerng ; Chao, Kuang Han ; Chen, Hsin Fu ; Wu, Ming Yih ; Yang, Yu Shih ; Lee, Tzu Yao. / Peritoneal natural killer cytotoxicity and CD25+CD3+ lymphocyte subpopulation are decreased in women with stage III-IV endometriosis. In: Human Reproduction. 1995 ; Vol. 10, No. 10. pp. 2671-2675.
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