Peritoneal interleukin-10 increases with decrease in activated CD4+ T lymphocytes in women with endometriosis

Hong Nerng Ho, Ming Yih Wu, Kuang Han Chao, Chin Der Chen, Shee Uan Chen, Yu Shih Yang

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

This study was performed to determine whether peritoneal T cells are suppressed in the CD4+ or CD8+ T cell subpopulation and whether they are Th1 or Th2 predominant in women with endometriosis. Immune cells in the peritoneal fluid (PF) were obtained from women undergoing laparoscopy for endometriosis or tubal ligation. Three-colour flow cytometry was utilized for immunophenotyping of peritoneal fluid mononuclear cells (PFMC). Concentrations of interleukin (IL)-4, IL-5 and interferon-γ (IFN-γ) produced by PFMC with and without mitogen stimulation and concentrations of IL-10 and IL-12 were measured in PF. The peritoneal T lymphocytes were predominantly of the Th1 type that produced much more IFN-γ but less IL-4 or IL-5 in women with or without endometriosis. The decrease in peritoneal lymphocytes was significant in the HLA-DR+CD4+CD3+ subpopulation and the concentrations of peritoneal IL-10 and IL-12 were significantly elevated in women with early stage endometriosis. There was impaired IL-5 production by PFMC after phytohaemagglutinin stimulation in women with advanced stage endometriosis. We concluded that the activated peritoneal CD4+ Th1 cells from the women with endometriosis were decreased in number. The suppression of these T cells may be due to the elevation of IL-10 and IL-12 in the peritoneal fluid.

Original languageEnglish
Pages (from-to)2528-2533
Number of pages6
JournalHuman Reproduction
Volume12
Issue number11
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Fingerprint

Th1 Cells
Ascitic Fluid
Endometriosis
Interleukin-10
Interferon-gamma
T-Lymphocytes
Interleukin-5
Interleukin-12
Interleukin-4
Tubal Sterilization
Immunophenotyping
Phytohemagglutinins
HLA-DR Antigens
Mitogens
Laparoscopy
Flow Cytometry
Color
Lymphocytes

Keywords

  • Endometriosis
  • Interferon-γ
  • Interleukins-4, -5, -10, -12
  • Peritoneal fluid
  • Th1 cells

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Peritoneal interleukin-10 increases with decrease in activated CD4+ T lymphocytes in women with endometriosis. / Ho, Hong Nerng; Wu, Ming Yih; Chao, Kuang Han; Chen, Chin Der; Chen, Shee Uan; Yang, Yu Shih.

In: Human Reproduction, Vol. 12, No. 11, 01.01.1997, p. 2528-2533.

Research output: Contribution to journalArticle

Ho, Hong Nerng ; Wu, Ming Yih ; Chao, Kuang Han ; Chen, Chin Der ; Chen, Shee Uan ; Yang, Yu Shih. / Peritoneal interleukin-10 increases with decrease in activated CD4+ T lymphocytes in women with endometriosis. In: Human Reproduction. 1997 ; Vol. 12, No. 11. pp. 2528-2533.
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