Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: An alternative to alveolaplasty

Y. L. Chen, P. K.T. Chen, L. B. Jeng, C. S. Huang, L. C. Yang, H. Y. Chung, S. C.N. Chang

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The regeneration of the periodontal attachment apparatus remains clinically challenging because of the involvement of three tissue types and the complexity of their relationship. Human recombinant bone morphogenic protein-2 (rhBMP-2) can accelerate the regeneration of bone and cementum and the insertion of periodontal ligament fibers but may lead to a deranged periodontal relationship, ankylosis and root resorption.This study evaluated a novel approach to regeneration of the periodontal attachment apparatus using a combination of ex vivo autologous bone marrow mesenchymal stem cells (MSCs) engineered by replication-defective adenovirus to express the BMP-2 gene and Pluronic F127 (PF127). Twenty-four periodontal defects were surgically created in 12 New Zealand white rabbits and randomly assigned to three experimental groups with MSCs: the advBMP-2 group; the advβgal group; the MSC group and one control group: PF127 only. The regenerated periodontal attachment apparatus was assessed histologically and the total regenerated bone volume was calculated from three-dimensional computed tomography analysis.This approach regenerated not only cementum with Sharpey's fiber insertion, but also statistically significant quantities of bone, re-establishing a more normal relationship among the components of the regenerated periodontal attachment apparatus, which is beneficial for the maintenance of periodontal health. Ex vivo gene transfer using stem cells as vectors may provide an advantage of slower BMP-2 release, increasing cementogenesis. There is regeneration of the periodontal attachment apparatus, whereas direct usage of the protein (rhBMP-2) yields unhinged periodontal relationship. Thus, this approach may represent an alternative means for periodontal alveolar bone graft in clinical settings.

Original languageEnglish
Pages (from-to)1469-1477
Number of pages9
JournalGene Therapy
Volume15
Issue number22
DOIs
Publication statusPublished - Aug 15 2008
Externally publishedYes

Fingerprint

UCON 50-HB-5100
Regeneration
Stem Cells
Mesenchymal Stromal Cells
Dental Cementum
Bone and Bones
Poloxamer
Genes
Cementogenesis
Root Resorption
Alveolar Bone Loss
Ankylosis
Periodontal Ligament
Bone Regeneration
Adenoviridae
Proteins
Bone Marrow
Tomography
Rabbits
Transplants

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene : An alternative to alveolaplasty. / Chen, Y. L.; Chen, P. K.T.; Jeng, L. B.; Huang, C. S.; Yang, L. C.; Chung, H. Y.; Chang, S. C.N.

In: Gene Therapy, Vol. 15, No. 22, 15.08.2008, p. 1469-1477.

Research output: Contribution to journalArticle

Chen, Y. L. ; Chen, P. K.T. ; Jeng, L. B. ; Huang, C. S. ; Yang, L. C. ; Chung, H. Y. ; Chang, S. C.N. / Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene : An alternative to alveolaplasty. In: Gene Therapy. 2008 ; Vol. 15, No. 22. pp. 1469-1477.
@article{0e38766bb4b6411181c2f84c3cf5a564,
title = "Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: An alternative to alveolaplasty",
abstract = "The regeneration of the periodontal attachment apparatus remains clinically challenging because of the involvement of three tissue types and the complexity of their relationship. Human recombinant bone morphogenic protein-2 (rhBMP-2) can accelerate the regeneration of bone and cementum and the insertion of periodontal ligament fibers but may lead to a deranged periodontal relationship, ankylosis and root resorption.This study evaluated a novel approach to regeneration of the periodontal attachment apparatus using a combination of ex vivo autologous bone marrow mesenchymal stem cells (MSCs) engineered by replication-defective adenovirus to express the BMP-2 gene and Pluronic F127 (PF127). Twenty-four periodontal defects were surgically created in 12 New Zealand white rabbits and randomly assigned to three experimental groups with MSCs: the advBMP-2 group; the advβgal group; the MSC group and one control group: PF127 only. The regenerated periodontal attachment apparatus was assessed histologically and the total regenerated bone volume was calculated from three-dimensional computed tomography analysis.This approach regenerated not only cementum with Sharpey's fiber insertion, but also statistically significant quantities of bone, re-establishing a more normal relationship among the components of the regenerated periodontal attachment apparatus, which is beneficial for the maintenance of periodontal health. Ex vivo gene transfer using stem cells as vectors may provide an advantage of slower BMP-2 release, increasing cementogenesis. There is regeneration of the periodontal attachment apparatus, whereas direct usage of the protein (rhBMP-2) yields unhinged periodontal relationship. Thus, this approach may represent an alternative means for periodontal alveolar bone graft in clinical settings.",
author = "Chen, {Y. L.} and Chen, {P. K.T.} and Jeng, {L. B.} and Huang, {C. S.} and Yang, {L. C.} and Chung, {H. Y.} and Chang, {S. C.N.}",
year = "2008",
month = "8",
day = "15",
doi = "10.1038/gt.2008.131",
language = "English",
volume = "15",
pages = "1469--1477",
journal = "Gene Therapy",
issn = "0969-7128",
publisher = "Nature Publishing Group",
number = "22",

}

TY - JOUR

T1 - Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene

T2 - An alternative to alveolaplasty

AU - Chen, Y. L.

AU - Chen, P. K.T.

AU - Jeng, L. B.

AU - Huang, C. S.

AU - Yang, L. C.

AU - Chung, H. Y.

AU - Chang, S. C.N.

PY - 2008/8/15

Y1 - 2008/8/15

N2 - The regeneration of the periodontal attachment apparatus remains clinically challenging because of the involvement of three tissue types and the complexity of their relationship. Human recombinant bone morphogenic protein-2 (rhBMP-2) can accelerate the regeneration of bone and cementum and the insertion of periodontal ligament fibers but may lead to a deranged periodontal relationship, ankylosis and root resorption.This study evaluated a novel approach to regeneration of the periodontal attachment apparatus using a combination of ex vivo autologous bone marrow mesenchymal stem cells (MSCs) engineered by replication-defective adenovirus to express the BMP-2 gene and Pluronic F127 (PF127). Twenty-four periodontal defects were surgically created in 12 New Zealand white rabbits and randomly assigned to three experimental groups with MSCs: the advBMP-2 group; the advβgal group; the MSC group and one control group: PF127 only. The regenerated periodontal attachment apparatus was assessed histologically and the total regenerated bone volume was calculated from three-dimensional computed tomography analysis.This approach regenerated not only cementum with Sharpey's fiber insertion, but also statistically significant quantities of bone, re-establishing a more normal relationship among the components of the regenerated periodontal attachment apparatus, which is beneficial for the maintenance of periodontal health. Ex vivo gene transfer using stem cells as vectors may provide an advantage of slower BMP-2 release, increasing cementogenesis. There is regeneration of the periodontal attachment apparatus, whereas direct usage of the protein (rhBMP-2) yields unhinged periodontal relationship. Thus, this approach may represent an alternative means for periodontal alveolar bone graft in clinical settings.

AB - The regeneration of the periodontal attachment apparatus remains clinically challenging because of the involvement of three tissue types and the complexity of their relationship. Human recombinant bone morphogenic protein-2 (rhBMP-2) can accelerate the regeneration of bone and cementum and the insertion of periodontal ligament fibers but may lead to a deranged periodontal relationship, ankylosis and root resorption.This study evaluated a novel approach to regeneration of the periodontal attachment apparatus using a combination of ex vivo autologous bone marrow mesenchymal stem cells (MSCs) engineered by replication-defective adenovirus to express the BMP-2 gene and Pluronic F127 (PF127). Twenty-four periodontal defects were surgically created in 12 New Zealand white rabbits and randomly assigned to three experimental groups with MSCs: the advBMP-2 group; the advβgal group; the MSC group and one control group: PF127 only. The regenerated periodontal attachment apparatus was assessed histologically and the total regenerated bone volume was calculated from three-dimensional computed tomography analysis.This approach regenerated not only cementum with Sharpey's fiber insertion, but also statistically significant quantities of bone, re-establishing a more normal relationship among the components of the regenerated periodontal attachment apparatus, which is beneficial for the maintenance of periodontal health. Ex vivo gene transfer using stem cells as vectors may provide an advantage of slower BMP-2 release, increasing cementogenesis. There is regeneration of the periodontal attachment apparatus, whereas direct usage of the protein (rhBMP-2) yields unhinged periodontal relationship. Thus, this approach may represent an alternative means for periodontal alveolar bone graft in clinical settings.

UR - http://www.scopus.com/inward/record.url?scp=55549097785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55549097785&partnerID=8YFLogxK

U2 - 10.1038/gt.2008.131

DO - 10.1038/gt.2008.131

M3 - Article

C2 - 18701911

AN - SCOPUS:55549097785

VL - 15

SP - 1469

EP - 1477

JO - Gene Therapy

JF - Gene Therapy

SN - 0969-7128

IS - 22

ER -